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EC number: 217-568-2
CAS number: 1889-67-4
Acute toxicity of the test item was assessed
with acute fish toxicity test on Zebrafish (Danio rerio) over an
exposure period of 96 hours in a semi-static system according to OECD
guideline 203 and EU method C.1. Due to the test item's poor water
solubility (maximum water solubility determined to be 0.08 mg/L), a
supersaturated solution was prepared thereby simulating worst-case
scenario conditions with this 96-hour semi-static acute toxicity test on
Zebrafish (Brachydanio rerio). Based upon the results from the
preliminary experiment, nominal concentrations of 7.9, 11.9, 17.8, 26.7,
40.0, 60.0, 80 and 100 % v/v saturated solution were investigated in the
main study. Most of the test concentrations were below the limit of
quantification. Thus, the 96-h LC50 value for the test item was
determined to be > 1000 mg/L based on the loading rate. The highest test
concentration causing no mortality within the test period (96-h LC0) was
determined to be between 100 – 1000 mg/L based on the loading rate. The
NOEC was determined to be in the range of 100 – 1000 mg/L (loading
rate), while the LOEC was determined to be 1000 mg/L (loading rate).
Under the conditions of the acute fish
toxicity study, the observed results were the following: The 96-h LC50
value was determined to be > 1000 mg/L (loading rate), and the 96-h NOEC
was determined to be in the range of 100 to 1000 mg/L (loading rate).
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