Registration Dossier
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EC number: 203-978-9 | CAS number: 112-50-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Bacterial Reverse Mutation data
Protocol | Strains tested | Positive and negative controls | Maximum tested dose | Result with and without metabolic activation | Reference |
Data for DEGME | |||||
OECD 471 | TA 98, 100, 1535, 1537, | Yes | 5mg/plate | Without: Negative | BASF AG (1989) |
Data for DEGEE | |||||
OECD 471 | TA 98, 100, 102, 1535, 1537 | Yes | 5mg/plate | Without: Negative | Aujoulat (1999) |
Data for DEGBE | |||||
Equivalent or similar to OECD 471 | TA 98, 100, 1535, 1537, 1538 | Yes | 20mg/plate | Without: Negative | Thompson (1984) |
Data for TEGME | |||||
Equivalent or similar to OECD 471 | TA 98, 100, 1535, 1537, 1538, E coliWP2 uvrA | Yes | 10mg/plate first experiment, 5mg/plate second experiment | Without: Negative | Muller (1988) |
Data for EGnPE | |||||
OECD 471 | TA 98, 100, 1535, 1537, E coliWP2 uvrA | Yes | 5mg/plate | Without: Negative | Harlan Laboratories Ltd (2011a) |
There are a further three negative studies available on TEGME and one further negative study on DEGBE and DEGME. None of these studies were used as key studies for their respective substances and are therefore not reported further here. All are completely consistent with the results presented in the table above.
Cytotoxicity data
Protocol | Species/strain | Positive and negative controls | Maximum tested dose | Result with and without metabolic activation | Reference |
Data for EGnPE | |||||
OECD 473 (CA) | Human Lymphocytes | Yes | 1mg/mL | Without: Negative | Harlan Laboratories Ltd (2011b) |
Data for DEGBE | |||||
Equivalent or similar to OECD 473 (CA) | CHO | Yes | 5mg/mL | Without: Negative | Thompson (1984) |
Data for TEGME | |||||
OECD 473 (CA)* | CHO | Yes | 5mg/mL | Without: Negative | Brooks (1992) |
*test material a formulated brake fluid comprising 30-40% of TEGME
Mammalian cell gene mutation data
Protocol | Strains tested-Target gene | Positive and negative controls | Maximum tested dose | Result with and without metabolic activation | Reference |
Data for EGnPE | |||||
OECD 476 | ML-L5178Y | Yes | 1mg/mL | Without: Negative | Harlan Laboratories Ltd (2011c) |
Data for DEGBE | |||||
Equivalent or similar to OECD 476 | CHO-HPGRT | Yes | 5mg/mL | Without: Negative | Dow (1987), Gollapudi (1993) |
Data for TEGME | |||||
EPA OTS 798.5300 | CHO-HPGRT | Yes | 5mg/mL | Without: Negative | Dow (1990a) |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Description of key information
Protocol | Species and strain used | Positive and negative controls | Maximum tested dose and exposure period | Result | Reference |
Data for DEGEE | |||||
Equivalent or similar to OECD 474 (micronucleus) | Mouse | Yes | 2mL/day for 3 days ip. Single limit dose used. | Negative | Berte (1986) |
Equivalent or similar to OECD 486 (UDS) | Rat | Yes | 2000mg/kg single limit dose po. | Negative | Clare (1996) |
Data for DEGBE | |||||
Equivalent or similar to OECD 475 (CA) | Mouse | Yes | 3300mg/kg single MTD dose po. | Negative | Gollapudi (1993) |
Data for TEGME | |||||
EPA OTS 798.5395 (MN) | Mouse | Yes | 2000mg/kg single dose po. | Negative | Dow (1990b) |
There is a negative drosophilia study using DEGBE. This was not used as key studies for DEGBE and therefore not reported further here.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Justification for classification or non-classification
The in vitro information covering the three required study end points for REACH of bacterial reverse mutation, cytogenicity in mammalian cells and mammalian cell gene mutation is consistently negative for all of the source substances. In the limited cases where there is any possible interpretation of equivocal results, in vivo data is available to provide re-assurance that all of the source substances are negative for mutagenic potential. The shared structural elements and common metabolic pathways across the source and target substances provides confidence in the reliability of using read across data for fulfilling the requirements for this end point for TEGEE and the data leads to the conclusion there is no evidence that TEGEE will have any mutagenic potential. Classification for this end point is not required.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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