4,5-dihydroxy-1,3-dimethylimidazolidin-2-one

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

GLP study with analytical test item concentration verification

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): about 3 months
- Length at study initiation (mean, range): 3.3 cm (3.0 - 3.7)
- Weight at study initiation (mean and range):0.30 g (0.21 - 0.48)
- Feeding during test: none
- Food type: Standard feed (TETRA MIN), on workdays generally additionally live artemia
- Amount: ad libitum

ACCLIMATION
- Conditions equivalent to housing
- Health during acclimation (any mortality observed): 0.1 % mortality during the last 14 days prior to test initiation

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

Total hardness: about 2.5 mM (about 250 mg/L CaCO3)
Acid capacity: about 5.5 mM

Test temperature:

21 - 23 °C

pH:

8.0 - 8.6

Dissolved oxygen:

7.0 - 8.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all-glass aquaria, 30 x 22 x 24 cm, 10 L fill volume
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.3 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated, char coal filtered
- Ca/Mg ratio: 9:1
- Intervals of water quality measurement: after 1, 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

In an Acute Toxicity Test in fish according to OECD guideline 203, the LC50 (96 h) was > 10000 mg/L.

Description of key information

LC50 (96 h) = >10000 mg/L (nominal, Danio rerio, OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 10 000 mg/L