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EC number: 220-562-2
CAS number: 2814-77-9
The toxic effects of C.I. Pigment Red 3 on survival,
growth, and reproduction of the earthwormEisenia fetidawere
assessed during a test period of eight weeks based on the OECD
Guidelines for Testing Chemicals, Test No. 222 (April 2004), the
International Standards11268‑2: 1998(E) and theGuideline “Richtlinien
für die Prüfung von Pflanzenschutzmitteln im Zulassungsverfahren“ Part
VI, 2-2, January 1994.
C.I. Pigment Red 3 was homogeneously mixed into artificial
soil at the following test concentrations: 62.5, 125, 250, 500 and
1000 mg per kg dry soil. Additionally, a control and a reference item
treatment (Derosal with 60% Carbendazim (w/w)) were tested in parallel.
Adult earthworms were exposed in treated soils for a period of four
weeks. After this period, the adults were removed from the test vessels
and the survival and growth rate were determined. The cocoons and
juvenile earthworms remained in the vessels for additional four weeks.
The reproduction rate was determined by counting the number of offspring
hatched from the cocoons after this additional test period of four weeks.
Summary of effects on earthworms exposed toC.I. Pigment Red 3:
In conclusion, taking into account the survival, growth and reproduction
rates of the exposed adult test organisms, the highest concentration of
C.I. Pigment Red 3 without toxic effects on Eisenia fetida (NOEC)
after the test period was determined to be 1000 mg/kg dry soil. This
value might even be higher but the highest test concentration tested was
1000 mg/kg dry soil. Accordingly, the lowest concentration with toxic
effects (LOEC) was >1000 mg/kg dry soil.
The reproduction rate of Eisenia fetida in the positive control
was completely inhibited. Thus, the results of the positive control
demonstrated satisfactory test conditions.
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