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EC number: 220-562-2
CAS number: 2814-77-9
cell density - limit test
Nominal concentration mg·L-1
Mean cell density in 1 mL
reduction of mean growth rate and percentual inhibition of mean yield –
curves and other details on results are in attached
test item, Pigment Red 4, was tested for growth inhibition on algae
test was performed according to method C.3. - Freshwater Algae and
Cyanobacteria, Growth Inhibition Test, Commission Regulation (EU)
preliminary test was performed in a range of the test item nominal
concentrations 1 – 100 mg·L-1.
highest inhibition of growth rate was 4.3 % and the highest inhibition
of yield was 18.8 % in the preliminary test. Based on low toxicity of
the test item found in the preliminary test (measured by growth rate),
the limit test was performed subsequently. Only the value of 72 hour –
ErC50 is needed for the classification and risk assessment of chemicals.
concentration of 100 mg·L-1 was tested in the limit test. The inhibition
of growth rate was 5.4 % and inhibition of yield was 18.3 %.
study was performed without the analytical determination of the test
item concentrations. The suitable analytical method for the
determination of the test item concentration is not available.
nominal concentrations were used for all evaluations and results.
hour – ErC50 > 100 mg·L-1 (nominal
hour – EyC50 > 100 mg·L-1 (nominal
hour – NOECr ˂ 1 mg·L-1 (nominal
hour – NOECy ˂ 1 mg·L-1 (nominal
determination of NOEC value was done by evaluation of the results from
preliminary test using statistical software ToxRat Professional Version
The inhibition of growth rate was 5.4 %
and the inhibition of yield was 18.3 % in the limit test. Therefore
exact value of ErC50 and EyC50 could not be calculated and the values of
EC are given in the form of a range.
The determination of NOEC value was done
by statistical software
ToxRat Professional Version 3.2.1
72 hour – ErC50 > 100 mg·L-1
72 hour – EyC50 > 100 mg·L-1
72 hour – NOECr < 1 mg· L-1 (nominal
72 hour – NOECy < 1 mg· L-1 (nominal
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