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EC number: 220-562-2
CAS number: 2814-77-9
toxicity to fish
test item, Pigment Red 4, was tested for acute toxicity on fish Poecilia
static limit test with a dispersed test item partly dissolved by using
ultrasonic device was conducted. The test was performed according to
method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and
96 h - LC50 > 100.0 mg.L-1
toxicity to aquatic invertebrates
test item, Pigment Red 4, was tested in acute immobilisation test on
Daphnia magna. The static test was performed according to method No. L
49 “Acute Toxicity for Daphnia, immobilization test. Chemical
formulations and waste waters”.
EC50 (48h) > 100 mg·L-1
to aquatic algae
test item, Pigment Red 4, was tested for growth inhibition on algae
test was performed according to method C.3. - Freshwater Algae and
Cyanobacteria, Growth Inhibition Test, Commission Regulation (EU)
72 hour – ErC50 > 100 mg·L-1 (nominal concentration)
Respiration Inhibition Test with activated sludge according to OECD
Guideline No. 209 was carried out as a limit test for both Monoazo Red
Pigments category members - C.I. Pigment Red 3 and C.I. Pigment5. The
test was performed under static conditions with the nominal limit
concentration of 1000 mg/L. The test item is not toxic at concentrations
≤ 1000 mg/L to activated sludge of a municipal sewage treatment plant.
toxicity to aquatic invertebrates
test item, Pigment Red 4, was tested in reproduction test on Daphnia
test was performed according OECD Test Guideline No. 211 - Daphnia magna
Reproduction Test. Adopted: October 2, 2012.
21 days – EC50 > 10 mg/L (nominal concentration)
toxicity to soil macroorganisms
toxic effects of a Monoazo Red Pigments category member - C.I. Pigment
Red 3 - on survival, growth, and reproduction of the earthworm Eisenia
fetida were assessed during a test period of eight weeks based on the
OECD Guidelines for Testing Chemicals, Test No. 222 (April 2004), the
International Standards11268‑2: 1998(E)
von Pflanzenschutzmitteln im Zulassungsverfahren“ Part VI, 2-2, January
and reproduction was not affected and the NOEC was at least > 1000 mg/kg
to Terrestrial Plants
emergence and seedling growth in an OECD 208 study with C.I. Pigment Red
3 for 10 different plant species did not reveal any toxicity and NOEC
was > 1000mg/kg soil. However, test with Beta Vulgaris is still ongoing
due to inconclusive test results.
no toxicity for any tested species of algae, aquatic invertebrates, fish,
microorganisms and soil organisms (Eisenida fetida)
could be found neither in acute nor in chronic tests, no environmental
classification is proposed.
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