7,7-dimethyl-3-oxa-6-azaoctan-1-ol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13th December 1982 to 17th December 1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results.

Qualifier:

equivalent or similar to guideline

Guideline:

other: Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibianhs. EPA-660/3-75-009

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

other: ASTM Standard Practice for Conducting Acute Toxicity Test with Fishes, Macroinvertebrates and Amphibians. (E729-80)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

Not applicable - no Analytical monitoring conducted.

Vehicle:

no

Details on test solutions:

- Route of Administration:
In aqueous solution

- Justification of Exposure Route:
Potential acute environmental harm will occur with compound in aqueous solution.

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name:
Fathead minnow

- Source:
Sea Plantations, Salem, Massachusetts

- Age at study initiation:
Fish were greater than 6 weeks and less than 2 years in age.

- Length at study initiation:
Mean body length (total): 4.61 cm (0.45 sd).

- Weight at study initiation:
Mean body weight: 0.86 grams (0.26 sd).

- Feeding during test
Not feed during test.

ACCLIMATION
- Acclimation period:
Greater than 14 days

- Acclimation conditions:
Fish were kept in aerated 200 gallon holding tanks with dilution water similar in characterisitics to that which was used during the test phase; minimum of 5 total volume additions to holding tanks per day.

- Type and amount of food:
Fish were fed approximately 5% of their body weight per day. Fish were fed Rangen Salmon Crumble (obtained from Ziegler Bros. Inc., Gardners, Pennsylvania).

- Feeding frequency:
- Health during acclimation:
Approximately 5% of Lot #3 died 30 days prior to start of the study of disease and/or other natural causes.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Observations were made after exposure at 24, 48, 72 nd 96 hours.

Hardness:

Hardeness of Dilution Water as mg/l CaCO3 (desired hardness - 125 - 175 mg/l CaCO3). Actual hardness - 160 mg/l.

Test temperature:

Range of Temperature (water) 22°C ± 1°C.

pH:

Range pH of dilution water - 7.4 - 8.2.

Dissolved oxygen:

Range throughout study = 6.5 - 9.1 O2 mg/l

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations for both the Range Finding study and the Definitive study: 89.0, 137.0, 211.0, 325.0 and 500.0 mg/l.

Details on test conditions:

TEST SYSTEM
- Aeration: None

- No. of organisms per vessel:
10

- No. of vessels per concentration (replicates):
2

- No. of vessels per control (replicates):
Not applicable

- No. of vessels per vehicle control (replicates):
1


OTHER TEST CONDITIONS
- Adjustment of pH:
No

- Photoperiod:
16 hours light, 8 hours darkness.

- Light intensity:
Not recorded

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Test results were used to derive the 96 hour LC50, defined as the calculated concentration of the test material causing 50% mortality in the populations of test fish at the specified time of exposure.
The statistical method used to calculate the LC50 values and their associated 95% confidence limits was a probit analyses based on Litchfield, J. T., F. Wilcoxon, 1949.

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
Initial test concentrations were assigned by the Study Director. Highest concentrations approximated the maximum solubility for the test compound.

- Range finding study
- Test concentrations:
Range finding tests were performed in order to define definative study concentrations.
Concentrations: 89.0, 137.0, 211.0, 325.0 and 500.0 mg/l.

- Results used to determine the conditions for the definitive study:
Test material concentration was the same for the definitive study as the range finding study.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

255.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% Confidence Limit: 218.0 - 298.9

Details on results:

- Behavioural abnormalities:
The following information summarises behavioural and coloration abnormalities observed during the study.

Surfacing
Total loss of Equilibrum
Loss of Swimming Ability
Lethargic

These behavioural abnormalities were observed at the test concentration 325.0 mg/l.

-Mortalities
There were no mortalities observed in the test concentrations 89.0, 137.0 and 211.0 mg/l throughout the study.

At the 24 hour observation period however, it was observed that within the 500.0 mg/l test material concentration group all 10 of the fish were deceased.
At the 96 hour observation period in the 325.0 mg/l test material concentration group in one replicate study all 10 fish were observed to be deceased, and in the 2nd replicate all but 1 fish was observed to be deceased.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

None reported.

Sublethal observations / clinical signs:

Table 1 - Mortality information at 24 hr Observation period

Nominal concentration (mg/l)	Control		89.0		137.0		211.0		325.0		500.0
Replicate No.	1	2	1	2	1	2	1	2	1	2	1	2
Number Surviving	10	10	10	10	10	10	10	10	3	2	0	0

Table 2 - Mortality informaiton at 96 hr Observation period

Nominal concentration (mg/l)	Control		89.0		137.0		211.0		325.0		500.0
Replicate No.	1	2	1	2	1	2	1	2	1	2	1	2
Number Surviving	10	10	10	10	10	10	10	10	0	1	-	-

- = No observation

Validity criteria fulfilled:

not specified

Conclusions:

The substance MRD-82-178 was exposed to the aquatic species Pimephales promelas in order to evaluate the acute toxicity. The LC50 was 255.3 mg/l.

Executive summary:

The study was conducted in order to evaluate the acute toxicity of MRD-82 -178 to the fathead minnow (Pimephales promelas).

The study was conducted in general accordance to appropriate ASTM Standards (E729 -80) and EPA Guidelines (EPA-66/3 -75 -009).

The LC₅₀ was 255.3 mg/l with 95% Confidence Limit: 218.0 - 298.9.

Description of key information

The acute toxicity of the substance to the fathead minnow (Pimephales promelas) was investigated in general accordance to appropriate ASTM Standards (E729 -80) and EPA Guidelines (EPA-66/3 -75 -009).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

255.3 mg/L