Registration Dossier
Registration Dossier
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EC number: 201-058-1 | CAS number: 77-78-1
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Relative sensitivities of forward and reverse mutation assays in|Salmonella typhimurium
- Author:
- Skopek TR, Liber HL, Kaden DA, Thilly WG
- Year:
- 1 978
- Bibliographic source:
- Proc Natl Acad Sci (USA) 75: 4465-4469. Cited in: EU RAR (2002)
- Reference Type:
- secondary source
- Title:
- EU Risk Assessment Report Dimethyl Sulphate
- Author:
- European Chemicals Bureau
- Year:
- 2 002
- Bibliographic source:
- EU RAR, Volume 12. Luxembourg 2002
Materials and methods
- Principles of method if other than guideline:
- Ames test
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Automatically generated during migration to IUCLID 6, no data available
- IUPAC Name:
- Automatically generated during migration to IUCLID 6, no data available
- Details on test material:
- - Name of test material (as cited in study report): dimethyl sulphate
No further data
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- without
- Metabolic activation system:
- not applicable
- Test concentrations with justification for top dose:
- 100, 200, 300 µM (12.6, 25.2, 37.8 µg/ml)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 60 min
Plating was performed in triplicates, no metabolic activiation; no additional data
DETERMINATION OF CYTOTOXICITY
- Method: cell survival - Evaluation criteria:
- no data
- Statistics:
- no data
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- in all strains evalueted
- Cytotoxicity / choice of top concentrations:
- other: 50% survivial at 100 mM
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Induction of test substance-induced His+ revertants was reported to be 2- to 30- fold over control for all strains used.
Applicant's summary and conclusion
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