Benzaldehyde, 2-hydroxy-5-nonyl-, oxime, branched

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no analytics

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): Lix 860 N (neat reagent); Nonylsalicylaldoxime
- Physical state: fluid
- Analytical purity: >99%
- Lot/batch No.: 070596
- Purity test date: September 1994
- Expiration date of the lot/batch: September 1997

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was directly added to the test vessel and homogenized for 10 seconds.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The test concentrations og 8 mg/L was slightly turbid.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Charles River Aquatics
- Feeding during test: no

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: SSNIFF M
- Health during acclimation (any mortality observed): < 5% mortality

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

20.5 - 21.7 °C

pH:

7.7 - 8.4

Dissolved oxygen:

>= 70% saturation

Nominal and measured concentrations:

Nominal: 1, 2, 4 and 8 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L aquaria with 5 L test solution
- Renewal rate of test solution (frequency/flow rate): every 24 h
- No. of organisms per vessel: according to guideline
- No. of vessels per concentration (replicates): according to guideline
- No. of vessels per control (replicates): according to guideline

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline

OTHER TEST CONDITIONS
- Photoperiod: 16h light: 8h dark

EFFECT PARAMETERS MEASURED: mortality was recorded after 2-4, 24, 48, 72 and 96h test duration.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: after 48 hours one fsih showed distrubed equilibrium
- Mortality of control: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: for the highest concentration a slight turbidity was observed at all observation times

Validity criteria fulfilled:

yes

Conclusions:

In a GLP study according to the EU Method C.1, the LC50 for acute toxicity to fish of the test substance was determined to be 1.1 mg /l.

Executive summary:

The acute toxicity of the test substance to fish was evaluated in a study according to EU Method C.1 under GLP conditions. In a semi-static study Brachydanio rerio was exposed for 96h. The test material was applied directly to the test vessels. Analytical monitoring in water was omited due to the very low water solubility. All validity criteria were fulfilled. The LC50 is determined to be 1.1 mg/L (nominal concentration based on loadings).

Description of key information

LC50 (96h) = 1.1 mg/L (nominal) for Danio rerio (EU method C.1)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.1 mg/L

Additional information

One study investigating the short-term toxicity of the test substance to freshwater fish is available. The study was performed according to GLP and EU method C.1 using Danio rerio as test organism (Scholz 1996). The test material was applied directly to the test vessels. Analytical monitoring in water was omited due to the very low water solubility. All validity criteria were fulfilled. The LC50 is determined to be 1.1 mg/L based on nominal concentration.