Registration Dossier

Administrative data

Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Study period:
3, 5, 7 and 21 September 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 111 (Hydrolysis as a Function of pH)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
Date of inspection: 30 August 2005 Date of Signature: 21 November 2005

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 3-Octadecyloxypropyl-N,N,N-trimethylammonium chloride
- Molecular formula (if other than submission substance): Not applicable
- Molecular weight (if other than submission substance): Not applicable
- Smiles notation (if other than submission substance): Not applicable
- InChl (if other than submission substance): Not applicable
- Structural formula attached as image file (if other than submission substance): Not applicable
- Substance type: white solid
- Physical state: solid
- Analytical purity: Not reported
- Impurities (identity and concentrations): Not reported
- Composition of test material, percentage of components: Not reported
- Isomers composition: Not reported
- Purity test date: Not reported
- Lot/batch No.: Exp. I-070518
- Expiration date of the lot/batch: Not reported
- Radiochemical purity (if radiolabelling): Not applicable
- Specific activity (if radiolabelling): Not applicable
- Locations of the label (if radiolabelling): Not applicable
- Expiration date of radiochemical substance (if radiolabelling): Not applicable
- Stability under test conditions: Not reported.
- Storage condition of test material: room temperature in the dark.
- Other: Not reported.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not reported.
Radiolabelling:
no

Study design

Analytical monitoring:
yes
Details on sampling:
Preparation of samples
Sample solutions were prepared in stoppered glass flasks at a nominal concentration of 2 g/l in the three buffer solutions.
The test solutions were split into individual vessels for each data point.
The solutions were shielded from light whilst maintained at the test temperature.
Preliminary test/Tier 1
Sample solutions at pH 4, 7 and 9 were maintained at 50.0 ± 0.5°C for a period of 5 days.
Analysis of sample solutions
The sample solutions were removed from incubation at various times and the pH of each solution recorded.
The concentration of the sample solution was determined by high performance liquid chromatography (HPLC) with mass selective (MS) detection.
Samples: Duplicate aliquots (A and B) of sample solution were diluted by a factor of 1000 using methanol.
Standards: Duplicate standard solutions of test material were prepared in methanol at a nominal concentration of 2.0 mg/l.

Buffers:
Potassium hydrogen phthalate at pH 4
Disodium hydrogen orthophosphate (anhydrous), potassium dihydrogen orthophosphate and sodium chloride at pH 7
Disodium tetraborate and sodium chloride at pH 9
Estimation method (if used):
Not applicable.
Details on test conditions:
TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: Not reported.
- Sterilisation method: Filtered through a 0.2 µm membrane filter.
- Lighting: The solutions were shielded from light.
- Measures taken to avoid photolytic effects: Not reported.
- Measures to exclude oxygen: The solutions were subjected to ultrasonication and degassing with nitrogen to minimise dissolved oxygen content.
- Details on test procedure for unstable compounds: Not applicable.
- Details of traps for volatile, if any: Not applicable.
- If no traps were used, is the test system closed/open: Closed.
- Is there any indication of the test material adsorbing to the walls of the test apparatus?: No.

TEST MEDIUM
Not applicable.

OTHER TEST CONDITIONS
- Adjustment of pH: Yes. pH 4, 7 and 9.
- Dissolved oxygen: Not reported.
Duration of testopen allclose all
Duration:
5 d
pH:
4
Initial conc. measured:
2.5 other: mmol dm-3
Duration:
5 d
pH:
7
Initial conc. measured:
>= 1 - <= 1.5 other: mmol dm-3
Duration:
5 d
pH:
9
Initial conc. measured:
>= 0.5 - 1 other: mmol dm-3
Number of replicates:
(Analysis of sample solution) Duplicate.
Positive controls:
no
Negative controls:
no
Statistical methods:
Not reported.

Results and discussion

Preliminary study:
Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life greater than 1 year at 25°C at pH 4 at 50.0 ± 0.5ºC (Table 1).
Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life greater than 1 year at 25°C at pH 7 at 50.0 ± 0.5ºC (Table 2).
Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life greater than 1 year at 25°C at pH 9 at 50.0 ± 0.5ºC (Table 3).
Test performance:
The linearity of the detector response with respect to concentration was assessed over the nominal concentration range of 0 to 5 mg/l. This was satisfactory with a correlation coefficient of 0.996 being obtained.
Transformation products:
not specified
Details on hydrolysis and appearance of transformation product(s):
Not applicable.
Dissipation DT50 of parent compoundopen allclose all
pH:
4
Temp.:
25 °C
DT50:
> 1 yr
Type:
not specified
pH:
7
Temp.:
25 °C
DT50:
> 1 yr
Type:
not specified
pH:
9
Temp.:
25 °C
DT50:
> 1 yr
Type:
not specified
Other kinetic parameters:
Not applicable.
Details on results:
See the preliminary study results.

Any other information on results incl. tables

Table 1 Concentrations of the test material at pH 4 at 50.0 ± 0.5ºC

 

Time (Hours)

0

2.4

72

120

Concentration (g/l)

2.03

2.08

2.07

1.97

% of initial

-

103

102

97.3

Table 2 Concentrations of the test material at pH 7 at 50.0 ± 0.5ºC

 

Time (Hours)

0

2.4

72

120

Concentration (g/l)

1.97

2.04

1.99

1.96

% of initial

-

104

101

99.9

Table 3 Concentrations of the test material at pH 9 at 50.0 ± 0.5ºC

 

Time (Hours)

0

2.4

72

120

Concentration (g/l)

2.18

2.11

2.04

2.01

% of initial

-

97.0

93.6

92.2

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The estimated half-lives at 25°C of the test material are > 1 year at pH 4, 7 and 9.
Executive summary:

Assessment of hydrolytic stability was carried out using Method C7 of Commission Directive 92/69/EEC and Method 111 of the OECD Guidelines for Testing of Chemicals, 13 April 2004. The results are as follows:

pH

Estimated half-life at 25°C

4

>1 year

7

>1 year

9

>1 year