Registration Dossier
Registration Dossier
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EC number: 218-915-0 | CAS number: 2280-49-1
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study according to GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-phenyl-N-[(trichloromethyl)thio]benzenesulphonamide
- EC Number:
- 218-915-0
- EC Name:
- N-phenyl-N-[(trichloromethyl)thio]benzenesulphonamide
- Cas Number:
- 2280-49-1
- Molecular formula:
- C13H10Cl3NO2S2
- IUPAC Name:
- N-phenyl-N-[(trichloromethyl)sulfanyl]benzenesulfonamide
- Test material form:
- other: solid
- Details on test material:
- N-Phenyi-N[( trichloromethyl)thio]benzenesulphonamide
Content: 99,8%
Constituent 1
Test animals
- Species:
- other: in vitro
- Strain:
- other: reconstructed human epidermis (RHS) model EST-1000
Test system
- Type of coverage:
- other: in vitro
- Preparation of test site:
- other: in vitro
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: The results of the concurrent negative control (NC, 0.9% NaCI) and positive control (PC, 5% SDS) demonstrated the viability (NC) and sensitivity (PC) of the test model.
- Duration of treatment / exposure:
- Undiluted N-Phenyi-N-[(trichloromethyl)thio]benzenesulphonamide was applied topically to the RHS model, i.e. 30 mg per insert (plus 30 µl 0.9%NaCI to moisten and ensure good contact with the skin; three replicates).
- Observation period:
- After an exposure period of 20 minutes, followed by a 42 hours post-treatment incubation period, the cell viability was measured to be 102 % (rounded) in the MTT (Methylthiazoletetrazolium) conversion assay.
- Number of animals:
- not applicable; triplicates
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: % cell viability
- Run / experiment:
- Cell viability [%]
- Value:
- 102.38
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: Non-Irritant
Any other information on results incl. tables
After an exposure period of 20 minutes, followed by a 42 hours post-treatment incubation period, the cell viability was measured to be 102 % (rounded) in the MTT (Methylthiazoletetrazolium) conversion assay.
The results of the concurrent negative control (NC, 0.9% NaCI) and positive control (PC, 5% SDS) demonstrated the viability (NC) and sensitivity (PC) of the test model.
Thus, the results show that no skin irritant property of N-Phenyi-N[( trichloromethyl)thio]benzenesulphonamide was determined by the assay used.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Executive summary:
Thus, the results show that no skin irritant property of N-Phenyl-N[( trichloromethyl)thio]benzenesulphonamide was determined by the assay used.
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