2-Oxepanone, polymer with 1,6-hexanediol

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

The study on a similar substance serves as data source for the endpoint study record "TARGET - Toxicity to aquatic algae-Capa 2043" and the justification for read-across is provided there.

Adequacy of study:

key study

Study period:

05/2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

This study was carried out according to OECD guideline no. 201. This study was also carried out according to GLP standards with the exception of the analytical measurements of test concentrations.

Justification for type of information:

Study serves as data source; read-across justification is found in Target record.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples for analysis were taken at test initiation (0 h) and test termination (72 h). Samples were only taken from the lowest and highest test concentrations (12.5 mg/L and 200 mg/L respectively).

Vehicle:

no

Details on test solutions:

Test solutions were prepared on the day of test initiation by adding 400 mg of the test substance to 400 ml of aerated M7-media and stirring for 10 minutes. The solution was then membrane filtered (0.45 µm) and used as stock solution. The nominal concentration of the stock solution was 580 mg/L of test substance. Test concentrations were made by dilution of the stock solution with growth media.

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

The test organism was a laboratory culture of the green alga Pseudokirchneriella subcapitata. The inoculum was taken from Toxicon's culture of P. subcapitata (NIVA CHL 1), which originates from NIVA, Oslo, Norway.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

No information provided

Hardness:

No information provided

Test temperature:

21-24 ± 2°C

pH:

0 hours: 7.6-8.1
72 hours: 6.5-9

Dissolved oxygen:

No information provided

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 12.5, 25, 50, 100 and 200 mg/L.
Measured concentrations were found to be within 20% of nominal concentrations.

Details on test conditions:

See methods below

Reference substance (positive control):

not specified

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

76 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

165 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

A 72-hour ErC50 of 165 mg/L was found for the response variable average specific growth rate when exposed to 2-oxepanone, polymer with 1,4-butanediol. The 72-hour NOEC was found to be 25 mg/L.

Results with reference substance (positive control):

No information provided

Reported statistics and error estimates:

For control and treatment groups, a mean value for growth rate along with variance estimate was calculated. The specific growth rate for each test concentration is related to the growth rate of the controls:

%Ir=(µc-µr)/µc * 100

Where:
%Ir= percent inhibition in average specific growth rate
µc= mean value for average specific growth rate (µ) in the control group
µr= average specific growth rate (µ) for the treatment replicate

Table 1: nominal NOEC and ErC values in mg/l, determined after 72 hours exposure to the test substance

2-Oxepanone, polymer with 1,4-butanediol (Capa 2043).

	72 h NOEC (mg/L)	72 h ErC10 (mg/L)	72 h ErC50 (mg/L)
Average specific growth rate, EC-values	25	76	165

Table 2: Calculated response variables for the treatment group and the control group (mean ± coefficient of variation) after 72 hours exposure to 2 -oxepanone, polymer with 1,4 -butanediol.

Concentration	72 h specific growth rate (µ) Mean ± CV (%)
Control	1.67  ± 2.4
12.5 mg/L	1.66 ± 1.2
25 mg/L	1.68 ± 1.8
50 mg/L	1.57 ±5.0
100 mg/L	1.46 ± 5.4
200 mg/L	0.59 ± 15

Table 3: Results of TOC determination of saturated 2-Oxepanone, polymer with 1,4-butanediol (Capa 2043) solutions and the measured concentrations in test solutions.

Nominal test concentration	TOC (mg/L)	Capa 2043 concentration (mg/L)	Nominal value± 20°C
12.5 mg/L (0 h)	7.7	12.6	10 -15
12.5 mg/L (72 h)	8.2	13.4	10 -15
200 mg/L (0 h)	120	197	160 -240
200 mg/L (72 h)	120	197	160 -240

Validity criteria fulfilled:

yes

Conclusions:

A 72-hour ErC50 of 165 mg/L was found for the response variable average specific growth rate when exposed to 2-oxepanone, polymer with 1,4-butanediol. The 72-hour NOEC was found to be 25 mg/L. As the ErC50 was found to be greater than 100 mg/L, the test substance can be regarded as non-toxic to the green algae Pseudokirchneriella subcapitata according to 67/548/EEC (KIFS 2005:7) and EC 1272/2008 (CLP).

Executive summary:

A 72-hour toxicity test was carried out to determine growth inhibition effects of 2-Oxepanone, polymer with 1,4-butanediol on the green alga Pseudokirchneriella subcapitata. The study was carried out according to OECD guideline no. 201. Test solutions were created by diluting a stock solution of the test substance in growth media. The duration of exposure was 72 hours and test solutions were kept on a shaking table at 21-24 ± 2 °C throughout. Illumination was approximately 100 µE m-2 s-1.m. Six replicates were used for the control and three for the test solutions.

The pH was measured at test initiation and also at test termination. Samples for analytical determination of test concentration were also taken from the highest and lowest concentrations at this time. These samples were analysed separately at a non-GLP laboratory.

The 72-hour ErC50 of 165 mg/L was found for the response variable average specific growth rate when exposed to 2-oxepanone, polymer with 1,4-butanediol. The 72-hour NOEC was found to be 25 mg/L. As the ErC50 was found to be greater than 100 mg/L, the test substance can be regarded as non-toxic to the green algae Pseudokirchneriella subcapitata according to 67/548/EEC (KIFS 2005:7) and EC 1272/2008 (CLP).

Reference 2

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

05/2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

This study was carried out according to OECD guideline no. 201. This study was also carried out according to GLP standards with the exception of the analytical measurements of test concentrations.

Justification for type of information:

It is considered appropriate to address the toxicity to aquatic algae data requirement for 2-oxepanone, polymer with 1,6-hexanediol by applying read-across from available experimental study data on 2-oxepanone, polymer with 1,4-butanediol. The source and target substances are low molecular weight polyester diols terminated by primary hydroxyl groups and are structurally similar in that they both contain 6-hydroxyhexanoic acid repeating units. The linking unit is 1,4-butanediol in 2-oxepanone, polymer with 1,4-butanediol and 1,6-hexanediol in 2-oxepanone, polymer with 1,6-hexanediol. The diols differ by two carbons in the aliphatic chain and are therefore sufficiently structurally similar to support a read-across approach. The source and target substances share similar physico-chemical properties in that both are liquids at room temperature and have similar water solubility and log Pow values. The substances are therefore sufficiently similar in terms of basic physico-chemical properties to support a read-across approach. A read-across justification document is attached in section 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

76 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

165 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

A 72-hour ErC50 of 165 mg/L was found for the response variable average specific growth rate when exposed to 2-oxepanone, polymer with 1,4-butanediol. The 72-hour NOEC was found to be 25 mg/L.

Results with reference substance (positive control):

No information provided

Reported statistics and error estimates:

For control and treatment groups, a mean value for growth rate along with variance estimate was calculated. The specific growth rate for each test concentration is related to the growth rate of the controls:

%Ir=(µc-µr)/µc * 100

Where:
%Ir= percent inhibition in average specific growth rate
µc= mean value for average specific growth rate (µ) in the control group
µr= average specific growth rate (µ) for the treatment replicate

Table 1: nominal NOEC and ErC values in mg/L, determined after 72 hours exposure to the test substance

2-Oxepanone, polymer with 1,4-butanediol (Capa 2043).

	72 h NOEC (mg/L)	72 h ErC10 (mg/L)	72 h ErC50 (mg/L)
Average specific growth rate, EC-values	25	76	165

Table 2: Calculated response variables for the treatment group and the control group (mean±coefficient of variation) after 72 hours exposure to 2 -oxepanone, polymer with 1,4 -butanediol.

Concentration	72 h specific growth rate (µ) Mean ± CV (%)
Control	1.67  ± 2.4
12.5 mg/L	1.66 ± 1.2
25 mg/L	1.68 ± 1.8
50 mg/L	1.57 ±5.0
100 mg/L	1.46 ± 5.4
200 mg/L	0.59 ± 15

Table 3: Results of TOC determination of saturated 2-Oxepanone, polymer with 1,4-butanediol (Capa 2043) solutions and the measured concentrations in test solutions.

Nominal test concentration	TOC (mg/L)	Capa 2043 concentration (mg/L)	Nominal value± 20°C
12.5 mg/L (0 h)	7.7	12.6	10 -15
12.5 mg/L (72 h)	8.2	13.4	10 -15
200 mg/L (0 h)	120	197	160 -240
200 mg/L (72 h)	120	197	160 -240

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of the target substance 2-oxepanone, polymer with 1,6-hexanediol to the green algae Pseudokirchneriella subcapitata is estimated to be a 72 h EC50 = 165 mg/L and a 72 h NOEC = 25 mg/L, based on read-across from a reliable study testing Capa-2043 (2-oxepanone, polymer with 1,4-butanediol).

Executive summary:

A 72-hour toxicity test was carried out to determine growth inhibition effects of 2-Oxepanone, polymer with 1,4-butanediol on the green alga Pseudokirchneriella subcapitata. The study was carried out according to OECD guideline no. 201. Test solutions were created by diluting a stock solution of the test substance in growth media. The duration of exposure was 72 hours and test solutions were kept on a shaking table at 21-24 ± 2 °C throughout. Illumination was approximately 100 µE m-2 s-1.m. Six replicates were used for the control and three for the test solutions. The pH was measured at test initiation and also at test termination. Samples for analytical determination of test concentration were also taken from the highest and lowest concentrations at this time. These samples were analysed separately at a non-GLP laboratory. The 72-hour ErC50 of 165 mg/L was found for the response variable average specific growth rate when exposed to 2-oxepanone, polymer with 1,4-butanediol. The 72-hour NOEC was found to be 25 mg/L. As the ErC50 was found to be greater than 100 mg/L, the test substance can be regarded as non-toxic to the green algae Pseudokirchneriella subcapitata according to 67/548/EEC (KIFS 2005:7) and EC 1272/2008 (CLP).

The basic physico-chemical properties and chemical structural of the source substance 2-oxepanone, polymer with 1,4-butanediol and the target substance 2-oxepanone, polymer with 1,6-hexanediol are considered to be sufficiently similar to support a read-across approach. Therefore, the target substance 2-oxepanone, polymer with 1,6-hexanediol is also concluded to have a 72-h ErC50 value of 165 mg/L and a 72-h NOEC value of 25 mg/L.    

Description of key information

The toxicity of the target substance 2-oxepanone, polymer with 1,6-hexanediol to the green algae Pseudokirchneriella subcapitata is estimated to be a 72 h EC50 = 165 mg/L and a 72 h NOEC = 25 mg/L, based on read-across from a reliable study testing Capa-2043 (2-oxepanone, polymer with 1,4-butanediol).

Key value for chemical safety assessment

EC50 for freshwater algae:

165 mg/L

EC50 for marine water algae:

25 mg/L

Additional information

A 72-hour toxicity test was carried out to determine growth inhibition effects of the read-across substance 2-Oxepanone, polymer with 1,4-butanediol on the green alga Pseudokirchneriella subcapitata. The study was carried out according to OECD guideline no. 201. Test solutions were created by diluting a stock solution of the test substance in growth media. The duration of exposure was 72 hours and test solutions were kept on a shaking table at 21-24 ± 2 °C throughout. Illumination was approximately 100 µE m-2 s-1 m. Six replicates were used for the control and three for the test solutions. The pH was measured at test initiation and also at test termination. Samples for analytical determination of test concentration were also taken from the highest and lowest concentrations at this time. These samples were analysed separately at a non-GLP laboratory. The 72-hour ErC50 of 165 mg/l was found for the response variable average specific growth rate when exposed to 2-oxepanone, polymer with 1,4-butanediol. The 72-hour NOEC was found to be 25 mg/l. As the ErC50 was found to be greater than 100 mg/l, the test substance can be regarded as non-toxic to the green algae Pseudokirchneriella subcapitata according to 67/548/EEC (KIFS 2005:7) and EC 1272/2008 (CLP).

 

The basic physico-chemical properties and chemical structural of the source substance 2-oxepanone, polymer with 1,4-butanediol and the target substance 2-oxepanone, polymer with 1,6-hexanediol are considered to be sufficiently similar to support a read-across approach. Therefore, the target substance 2-oxepanone, polymer with 1,6-hexanediol is also concluded to have a 72-h ErC50 value of 165 mg/L and a 72-h NOEC value of 25 mg/L.