Phenetole

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference 1

Reference Type:

publication

Title:

THE TOXICITY AND TOXIC MANIFESTATIONS OF 2,2-BIS-(p-CHLOROPHENYL)-1,1,1-TRICHLOROETHANE (DDT) AS INFLUENCED BY CHEMICAL CHANGES IN THE MOLECULE: A Contribution to The Relation Between Chemical Constitution and Toxicological Action

Author:

W. F. von OETTINGEN and N. E. SHARPLESS

Year:

1946

Bibliographic source:

J Pharmacol Exp Ther December 1946 88:400-413

Reference 2

Reference Type:

publication

Title:

Journal of Pharmacology and Experimental Therapeutics

Year:

1946

Bibliographic source:

JPETAB Journal of Pharmacology and Experimental Therapeutics. (Williams & Wilkins Co., 428 E. Preston St., Baltimore, MD 21202) V.1- 1909/10- Volume(issue)/page/year: 88,400,1946

Reference 3

Reference Type:

review article or handbook

Title:

RTECS Number : SI7700000

Author:

RTECS database

Year:

2012

Bibliographic source:

RTECS (Registry of Toxic Effects of Chemical Substances); JPETAB Journal of Pharmacology and Experimental Therapeutics. (Williams & Wilkins Co., 428 E. Preston St., Baltimore, MD 21202) V.1- 1909/10- Volume(issue)/page/year: 88,400,1946

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

The toxicity and toxic manifestations of phenetole(as influenced bt chemical changes in DDT) in mice.

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Phenetole

EC Number:

203-139-7

EC Name:

Phenetole

Cas Number:

103-73-1

Molecular formula:

C8H10O

IUPAC Name:

ethoxybenzene

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study report): Phenetole
- Molecular Formula: C8H10O
- Molecular Weight: 122.17 g/mol
- Substance type: Organic
- Physical state: Liquid

Test animals

Species:

mouse

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source:Not available
- Age at study initiation:Not available
- Weight at study initiation:20-30 gm
- Fasting period before study:Not available
- Housing:individual cages
- Diet (e.g. ad libitum):Purina dog chow(ad libitum)
- Water (e.g. ad libitum):water(ad libitum)
- Acclimation period:Not available

ENVIRONMENTAL CONDITIONS
- Temperature (°C):Not available
- Humidity (%):Not available
- Air changes (per hr):Not available
- Photoperiod (hrs dark / hrs light):Not available

IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle:5,10 or 25 percent solutions in olive oil
- Amount of vehicle (if gavage):Not available
- Justification for choice of vehicle:Not available
- Lot/batch no. (if required):Not available
- Purity:Not available

MAXIMUM DOSE VOLUME APPLIED:Not applicable

DOSAGE PREPARATION (if unusual):5,10 or 25 percent solutions in olive oil

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose:Not available

Doses:

1200,1400,1600,1800,2000 & 2200 mg/kg

No. of animals per sex per dose:

1200mg/kg-10
1400mg/kg-10
1600mg/kg-10
1800mg/kg-10
2000mg/kg-10
2200mg/kg-10

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing:no data
- Necropsy of survivors performed: no data
- Other examinations performed: clinical signs-incidence of tremors,convulsions & fatalities noted

Statistics:

Not availble

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

2 200 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 50 percent mortality observed

Mortality:

50 percent mortality observed at 2200 mg/kg,whereas 80 percent mortality was seen at dose 2000 mg/kg and 10 percent mortality was seen at 1600 & 1800 mg/kg respectively.

Clinical signs:

other: Shortly after the administration the animals became depressed and with larger doses they passed into deep necrosis in 2-3 hrs and majority of animals died in a comatose condtion within 1-2 days.

Gross pathology:

Not available

Other findings:

Not available

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute oral median lethal dose (LD50) of phenetole in mouse was found to be 2200 mg/kg of body weight as 50 percent mortality was observed at this dose.

Executive summary:

In the study the acute oral toxicity of test compound was determined in white mice by orally administering the test compound in olive oil at doses 1200, 1400,1600,1800,2000 & 2200 mg/kg and observed for clinical signs and mortality for 1 week. The acute oral median lethal dose (LD50) of test chemical in mouse was found to be 2200 mg/kg of body weight (50 percent mortality observed), 80 percent mortality was seen at dose 2000 mg/kg and 10 percent mortality was seen at 1600 & 1800 mg/kg respectively. Thus considering the CLP criteria for classification it can be assessed that the given test chemical is non toxic via oral route as it exceeds the criteria of 2000 mg/kg bw for the substance to be classified as toxic.