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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: early study report, but basic information given

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1968
Report date:
1968

Materials and methods

Principles of method if other than guideline:
BASF-internal standard
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Trilon AS
IUPAC Name:
Trilon AS
Constituent 2
Chemical structure
Reference substance name:
Nitrilotriacetic acid
EC Number:
205-355-7
EC Name:
Nitrilotriacetic acid
Cas Number:
139-13-9
Molecular formula:
C6H9NO6
IUPAC Name:
2-[bis(carboxymethyl)amino]acetic acid
Details on test material:
H3.NTA ("TRILON AS"), solid

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of the same animal
Amount / concentration applied:
Test substance was applied as powder (1 x 50 mm3).
Duration of treatment / exposure:
24 hours
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
2

Results and discussion

In vivo

Results
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 8 d
Score:
3
Max. score:
3
Reversibility:
not reversible

Any other information on results incl. tables

Slight corneal opacity and severe edema after 1 h, erythema became severe while edema began to reverse after 24 h; slight corneal opacity persisted for 8 d while all other signs were reversible.

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information