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Short-term toxicity to fish

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Description of key information

1,4-bis(tert-butylperoxyisopropyl)benzene has no effect at a 100% v/v saturated solution (100 mg/L). 

Key value for chemical safety assessment

Additional information

The acute toxicity of 1,4-bis(tert-butylperoxyisopropyl)benzene to zebra fish has been investigated according to OECD 203 guideline in a GLP study. As no effect was observed during the preliminary test, a definitive limit-test at 100 mg/L was conducted and gave a 96-Hour LC50 of greater than 100% v/v saturated solution. The No Observed Effect Concentration was 100% v/v saturated solution.

To conclude, this study showed no toxic effects at saturation.

 

This study was performed in order to confirm the Read-Across approach between 1,4-bis(tert-butylperoxyisopropyl)benzene and 1,3-bis[1-(tert-butylperoxy)-1-methylethyl]benzene (see document attached to IUCLID) for which no effect was also observed with a similar study (summary below):

 

1,3-bis[1-(tert-butylperoxy)-1-methylethyl]benzene was tested in an acute toxicity test with Poecilia reticulata (guppy) under semi-static conditions in accordance with EEC method C.1. (1984) and OECD Test Guideline 203 (1984). At all concentrations tested the fish developed serious deviations in behaviour and appearance during the test period, such as reduced activity, convulsive movements and floating on the back. Most of the fish developed a thick white stomach.

After 96 hours, no mortality was observed up to and including 560 mg/L and 100% mortality was observed at 1000 mg/L after 96 hours and at1800 mg/L after 24 hours.

 

The LC50(96 h) was calculated to be 750 mg/L, with 95% confidence limits of 360 and 1570 mg/L.

However, given that during the test a layer of test substance remained visible on the surface of the test media in all test concentrations, the LC50value should be handled with care. Moreover no analytical monitoring was performed during the test. Based upon the fact that no mortality was observed this test can be considered as valid with restrictions.