2,2'-oxydi(ethylamine)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Following the read across strategy, this endpoint is covered by a key study performed with the structural analogue BDMAEE (N, N, N', N'-tetramethyl-2,2'-oxybis(ethylamine)). The justification for read across is attached in IUCLID section 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 720 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Validity criteria fulfilled:

yes

Conclusions:

No reliable data is available with the test substance. Data generated with the analogue substance BDMAEE is used for ednpoint coverage. Under the conditions of this study, the 30 minute EC20 was >720 mg/L (nominal) based on respiration rate in industrial activated sludge. The same is assumed to be correct for the test substance.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Documentation of the older study falls short of today´s requirements, but corresponds to the standards at the time they were conducted; the quality of the study performance is nevertheless not influenced. Sufficient data suitable for assessment. Inoculum taken from industrial source.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

see below

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge, industrial

Details on inoculum:

source: industrial waste water treatment plant of BASF AG, production site Ludwigshafen
final test concentration: 1 g/L dry weight

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test temperature:

room temperature: 20-25 °C

pH:

no details

Nominal and measured concentrations:

Dilution series from stock solutions (1000 mg/L nominal) resulted in the following concentrations: 720 and 360 mg/L (nominal).

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, Erlenmeyer-flask (250 ml nominal), liquid volume: 250 mL
- Aeration: sparged with air
- No. of vessels per concentration (replicates; test substance, TS): 1
- No. of vessels per control (replicates; blanc, BC): 0
- No. of vessels per concentration (replicates; positive control, reference substance, RS): 0

TEST MEDIUM / WATER PARAMETERS
- Culture medium: OECD test medium

OTHER TEST CONDITIONS
- Adjustment of pH: no pH adjustments during test

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
O2: on line measurement for approx. 10 min
pH: no data

TEST CONCENTRATIONS
- Spacing factor for test concentrations: <3.2
- Test item concentrations:720 and 360 mg/L (nominal)

Test conditions
Reference substance: not tested in parallel

Reference substance (positive control):

no

Key result

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 720 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

Effect concentrations exceeding solubility of substance in test medium: no

Reference value for blanc (mean value): 27 mg/L*h

Test concentration	Respiration rate	Respiration change
[mg/L]	[mg O2/L/h]	[%]
360	31	15
720	30	11

Respiration was stimulated by the test substance.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study, the 30 minute EC20 was >720 mg/L (nominal) based on respiration rate in industrial activated sludge

Description of key information

Following the read-across strategy, it is considered appropriate to cover this endpoint by a key study performed with the structural analogue BDMAEE (N, N, N', N'-tetramethyl-2,2'-oxybis(ethylamine)). A study performed under GLP conditions and according to OECD 209 showed a 30 min. EC20 >720 mg/L (nominal) based on respiration rate in industrial activated sludge.

Additional information

No reliable data is available with the test substance. Data generated with the analogue substance BDMAEE is used for endpoint coverage. A study performed under GLP conditions and according to OECD 209 showed a 30 min. EC20 >720 mg/L (nominal) based on respiration rate in industrial activated sludge. The same is assumed to be correct for the test substance.