3,3'-thiodi(propionic acid)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP - Guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

At the start and end of exposure, 0.75 mL of test liquid was sampled from 1 test container of each test group, and after acetonitrile was equally added and stirred into this, it was analyzed using HPLC. The test material concentration of each test liquid was quantified from the ratio with the standard solution peak area to find the geometric mean.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Japanese name: Oomijinko
- Source: Environment Agency, National Institute for Environmental Studies
- Age at study initiation: Male juvenile (born within 24 hours)
- Feeding during test: not fed during test

ACCLIMATION
- Acclimation period: rearing in conditioned water as used for test, prepared according to OECD Guideline 211; 1 organism/80 mL (25 organisms/2 L) or less
- Acclimation conditions: same
- Type and amount of food: Chlorella vulgaris (monocellular green algae)
- Feeding frequency: 0.2 mg C/organism/day
- Health during acclimation: Parent mortality rate 2 weeks prior to start of exposure: < 5%

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Approx. 250 mg/L (CaCO3 conversion)

Test temperature:

20 +/- 1°C

pH:

The test liquid pH was not adjusted. During study: see table 3 . For test substance concentration 100 mg/L pH was only 5.1 and therewith outside the suitable range. This was thought to be due to the test material because the pH was low for a high concentration group at preparation.

Dissolved oxygen:

Saturated concentration of 60% or more

Nominal and measured concentrations:

Nominal concentrations: 0, 10, 18, 32, 56, 100 mg/L
Measured concentrations (geometric mean during 48h): 9.82, 17.3, 31.5, 55.0, 97.2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: 100 mL glass beaker
- Aeration: no
- No. of organisms per vessel: 5 organisms/container
- No. of vessels per concentration (replicates): 4 containers/test group
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The conditioned water and Elendt M4 stated in OECD Chemical Test Guideline No.211 “Daphnia Magna
Reproduction Test” were used.
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod and light intensity: Indoor light, 16 hours lit (800 lux or less)


EFFECT PARAMETERS MEASURED: Immobility

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: yes

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 97.2 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

73.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 64.3-83.2 mg/L

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

55 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

31.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 97.2 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 97.2 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: none

Table 1: Measured Concentrations of the Test Substance in Test Water (Semi-Static Condition)

Nominal Concentration (mg/L)	Measured concentration (mg/L)				Geometric Mean During 48 Hours (mg/L)
	0 Hour New	Percent of Nominal	48 Hours Old	Percent of Nominal
Control	< 0.08	--	0.08	--	--
10.0	9.88	99	9.77	98	9.82
18.0	17.5	97	17.2	96	17.3
32.0	31.8	99	31.2	98	31.5
56.0	56.0	99	54.4	97	55.0
100	98.7	99	95.8	96	97.2

 

 

Table 2: The Number of Immobilised Daphnia magna (Percent Immobility)

Nominal Concentration (mg/L)	Mean^aMeasured Concentration (mg/L)	Cumulative Number of Immobilized Daphnia (Percent Immobility)
		24 Hours	48 Hours
Control	------	0 (0)	0 (0)
10.0	9.82	1 (5)	1 (5)
18.0	17.3	0 (0)	1 (5)
32.0	31.5	0 (0)	0 (0)
56.0	55.0	0 (0)	2 (10)
100	99.1	2 (10)	18 (90)

a: geometric mean

 

 

Table 3: pH Values

Nominal Concentration (mg/L)	Mean^aMeasured Concentration (mg/L)	pH
		0 Hour New	48 Hours Old
Control	---	8.4	8.1
10.0	9.82	8.3	7.8
18.0	17.3	7.3	7.2
32.0	31.5	6.7	6.8
56.0	55.0	6.2	6.3
100	97.2	5.1	5.0

a: geometric mean

new: freshly prepared test solutions

old: test solutions after 48 hours exposure

 

Table 4: Temperature

Nominal Concentration (mg/L)	Mean^aMeasured Concentration (mg/L)	Temperature (°C)
		0 Hour New	48 Hours Old
Control	---	19.9	20.0
10.0	9.82	19.9	20.0
18.0	17.3	19.9	19.9
32.0	31.5	20.0	19.9
56.0	55.0	20.0	19.9
100	97.2	20.0	19.9

a: geometric mean

New: freshly prepared test solutions

Old: test solutions after 48 hours exposure

 

 

Table 5: Dissolved Oxygen Concentrations

Nominal Concentration (mg/L)	Mean^aMeasured Concentration (mg/L)	Dissolved Oxygen Concentration (mg/L)
		0 Hour New	48 Hours Old
Control	---	8.8	8.5
10.0	9.82	8.8	8.5
18.0	17.3	8.8	8.5
32.0	31.5	8.8	8.5
56.0	55.0	8.8	8.7
100	97.2	8.8	8.7

a: geometric mean

New: freshly prepared test solutions

Old: test solutions after 48 hours exposure

Description of key information

The EC50 (48h) is 73.1 mg/L (geometric mean of measured concentrations).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

73.1 mg/L

Additional information

During the acute static immobilisation test with Daphnia magna according to OECD 202 90% of the daphnids were immobilised at the highest tested concentration of 100 mg/L (nominal). The EC50 was determined to be 73.1 mg/L (geometric mean of measured concentrations). Thus, the substance shows a toxicity to Daphnia magna under conditions tested.