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EC number: 220-778-7
CAS number: 2896-70-0
A GLP study was performed in accordance with OECD guideline 474 in order
to assess the potential of 2,2,6,6,-tetramethyl-4-oxopiperdinooxy to
produce damage to chromosomes or aneuploidy when administered to mice.
A range-finding study was performed to identify suitable dose levels and
route of administration.
The micronucleus study was conducted via the intraperitoneal route in
groups of 10 mice (five male/ five female) at the maximum tolerated dose
(MTD) 500 mg/kg and 250 and 125 mg/kg at the lower doses. The
test animals were sacrificed 24 or 48-hours after treatment, the bone
marrow extracted and smear preparations made and stained. Polychromatic
and monochromatic erythrocytes (PCE and NCE) were scored for the
presence of micronuclei. Further groups of mice were treated with a
single oral dose of arachis oil or cylclophosphamide to serve as vehicle
and positive controls respectively.
The results showed no significant increase in the incidence of
micronucleated PCE in animals dosed with the test material when compared
to the concurrent vehicle controls. No statistically
significant decrease was observed in the PCE/NCE ratio in either the 24
or 48-hour treatment groups when compared to the control. The
observation of clinical signs in the 500 mg/kg dose group and the
premature deaths observed in the 500 mg/kg 48 hour dose group were taken
to indicate that systemic absorption had occurred.
The positive control produced a marked increase in the frequency of
micronucleated PCEs demonstrating the sensitivity of the test system.
Under the conditions of the test, 2,2,6,6-tetramethyl-4-oxpiperidinooxy
was considered to be non- gentoxic.
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