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Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Referenced sources in an international report.

Data source

Reference
Reference Type:
review article or handbook
Title:
Hydrogen Chloride, SIDS Initial Assessment Report For SIAM 15
Author:
OECD
Year:
2002
Bibliographic source:
UNEP PUBLICATIONS

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Hydrogen chloride
EC Number:
231-595-7
EC Name:
Hydrogen chloride
Cas Number:
7647-01-0
IUPAC Name:
chloride
Constituent 2
Reference substance name:
Hydrochloric acid
IUPAC Name:
Hydrochloric acid
Test material form:
not specified
Details on test material:
10% hydrochloric acid in water.

Test animals / tissue source

Species:
rabbit
Strain:
other: New Zealand

Test system

Vehicle:
water
Controls:
not specified
Amount / concentration applied:
Amount applied: 100 µl of 10% hydrochloric acid
Duration of treatment / exposure:
96h
Observation period (in vivo):
96h
Number of animals or in vitro replicates:
6 animals per sex per dose
Details on study design:
100 µL of 10% hydrochloric acid in water was applied into the lower conjunctival sac.
Scoring method used: Draize scoring criteria.
Tool used to assess score: Application of one drop of 2 % sodium fluorescein was given before visual scoring of percentage corneal damage.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 4h
Score:
1.9
Max. score:
3
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24h
Score:
2.8
Max. score:
3
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 48h
Score:
3
Max. score:
3
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 72h
Score:
3
Max. score:
3
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 96h
Score:
2.9
Max. score:
3
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 4h
Score:
1
Max. score:
4
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24h
Score:
1.7
Max. score:
4
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 48h
Score:
2.3
Max. score:
4
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 72h
Score:
2.3
Max. score:
4
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 96h
Score:
2
Max. score:
4
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 4h
Score:
1
Max. score:
2
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24h
Score:
1.3
Max. score:
2
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 48h
Score:
1.7
Max. score:
2
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 72h
Score:
1.7
Max. score:
2
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 96h
Score:
1.7
Max. score:
2
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 4h
Score:
1.3
Max. score:
4
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24h
Score:
2
Max. score:
4
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 48h
Score:
2.7
Max. score:
4
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 72h
Score:
3.3
Max. score:
4
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 96h
Score:
3
Max. score:
4
Other effects:
Mean surface of Corneal Damage (100% maximum)/Time after end of application (h)
15/4h
59/24h
49/48h
42/72h
28/96h

Any other information on results incl. tables

Reference: Jacobs, G. A., OECD Eye Irritation Tests on Two Strong Acids, J. Am. Coll. Toxicol., 11, 6 (1992)

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Hydrochloric acid was classified as ‘Risk of serious damage to eyes’ according to the EC Criteria (mean over 24/48/72 Hrs of iritis>1.5).
HCl is present in the mixture Azacyclonol HCl in a content of 11% which is between the range of 10 – 25 % that determines a classification of Eye irritant Cat.2, H319.