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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
full method description is not available
GLP compliance:
no
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Div. Lab. Vers.
- Expiration date of the lot/batch: 11/2003

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room Temperature
- Solubility and stability of the test substance in the solvent/vehicle: <0.2 g/l (in water)
Analytical monitoring:
not specified
Details on sampling:
no data
Vehicle:
not specified
Details on test solutions:
no data
Test organisms (species):
other: bacteria
Details on inoculum:
no data
Test type:
not specified
Water media type:
not specified
Total exposure duration:
3 h
Post exposure observation period:
none
Hardness:
no data
Test temperature:
no data
pH:
8-8.1
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
Nominal: 64, 160, 400 and 1000 mg/L
Details on test conditions:
no data
Reference substance (positive control):
yes
Remarks:
3.5-Dichlorophenol
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: Due to its physico-chemical properties the test substance was not completely soluble in the test medium. The reported results are related to the amount of the test substance given into the test system.
Details on results:
none
Results with reference substance (positive control):
3h IC50 of reference substance (3,5 Dichlorophenol), was 13 mg/L.
Reported statistics and error estimates:
no data

Table:

Test substance (mg/L)

pH after 3 h

O2 consumption (in cm)

Time (in cm)

mg O2 pro min./L

% of control

% inhibition

1000

8.1

7.1

7.0

0.81

99.91

0.09

400

8.1

6.6

6.8

0.78

95.61

4.39

160

8.1

6.3

6.0

0.84

103.43

0.00

64

8.0

5.8

5.6

0.83

102. 03

0.00

Validity criteria fulfilled:
not specified
Conclusions:
The IC20 and IC50 (3 h) values of the test substance were determined to be >1000 mg/L (i.e. 960 mg a.i./L).

Executive summary:

The bacterial toxicity potential of FAT 93450/A was assessed in an activated sludge respiration inhibition test conducted according to OECD Guideline 209. The test substance was not completely soluble in the test medium. The reported results are based on nominal concentrations. The % inhibition observed was 0.09, 4.39, 0 and 0 % at 1000, 400, 160 and 64 mg/L concentration, respectively. Hence, the 3 h IC20 and IC50 values of the test substance were determined to be >1000 mg/L (i.e. 960 mg a.i./L). 

Description of key information

The bacterial toxicity potential of FAT 93450/A was assessed in an activated sludge respiration inhibition test conducted according to OECD Guideline 209. The test substance was not completely soluble in the test medium. The reported results are based on nominal concentrations. The % inhibition observed was 0.09, 4.39, 0 and 0 % at 1000, 400, 160 and 64 mg/L concentration, respectively. Hence, the 3 h IC20 and IC50 values of the test substance were determined to be >1000 mg/L (i.e. 960 mg a.i./L). 

Key value for chemical safety assessment

EC50 for microorganisms:
960 mg/L

Additional information