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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
not specified
Analytical monitoring:
not specified
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Details on test conditions:
Nominal: 100, 1000 and 10000 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
650 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No observed effect concentration: 100 mg/L
Maximum concentration tested causing no mortality: 100 mgIL
Minimum concentration tested causing 100 % mortality: 10000 mg/L

The below symptoms were monitored after 1, 4, 24, 48, 72, 96 hours. Symptoms like apathy, swimming near water surface, narcotic like state, swimming near the bottom

NOMINAL CONC.

(MG/L)

 1H

4H 

24H 

48H 

72H 

96H 

 100

 

 

 

 

 

 

 1000

       CDA  CNA CN 
 10000            

A= apathy

C= swimming near water surface,

D= narcotic like state,

N- swimming near the bottom

Results:

 

Nominal conc

Number of fish

Dead fish after

(mg/L)

 

1 h

4h

24h

48h

72h

96h

100

10

0

0

0

0

0

0

1000

10

0

0

0

1

3

7

10000

10

0

10

10

10

10

10

Validity criteria fulfilled:
not specified
Conclusions:
The 96h LC50 for zebra fish (Danio rerio) was 650 mg/L (value of interpolation).
Executive summary:

The short term toxicity of the test substance was assessed in a study conducted according to a methodology equivalent to OECD Guideline 203. Groups of 10 Zebra fish each, were exposed to test substance at 100, 1000 or 10000 mg/L for a duration of 96 h. The number of surviving fish was recorded at 1, 4, 24, 48, 72 and 96 h.

There was no mortality at 100 mg/L. 10 out of 10 fish died within 96 h at 10000 mg/L, while 7 out of 10 fish died after 96h at 1000 mg/L.

Based on the findings of the study, the 96 h LC50 for zebra fish (Danio rerio) was 650 mg/L (value of interpolation) and NOEC value (96h) was 100 mg/L.

Description of key information

The short term toxicity of the test substance was assessed in a study conducted according to a methodology equivalent to OECD Guideline 203. Groups of 10 Zebra fish each, were exposed to test substance at 100, 1000 or 10000 mg/L for a duration of 96 h. The number of surviving fish was recorded at 1, 4, 24, 48, 72 and 96 h. There was no mortality at 100 mg/L. 10 out of 10 fish died within 96h at 10000 mg/L, while 7 out of 10 fish died after 96h at 1000 mg/L. Based on the findings of the study, the 96h LC50 for zebra fish (Danio rerio) was 650 mg/L (value of interpolation) and NOEC value (96h) was 100 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
650 mg/L

Additional information