Benzyldimethyl[2-[(1-oxoallyl)oxy]ethyl]ammonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29/09/1993-06/10/1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzyldimethyl[2-[(1-oxoallyl)oxy]ethyl]ammonium chloride

EC Number:

256-283-8

EC Name:

Benzyldimethyl[2-[(1-oxoallyl)oxy]ethyl]ammonium chloride

Cas Number:

46830-22-2

Molecular formula:

C14H20NO2.Cl

IUPAC Name:

benzyldimethyl[2-(prop-2-enoyloxy)ethyl]azanium chloride

Details on test material:

- Name of test material (as cited in study report): ADAMQUAT BZ 80,
- Physical state: liquid,
- Analytical purity: 78.5%.
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: OP/611/613/615/616
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: at room etmperature and away from the light

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Details on test solutions:

The initial solution of ADAMQUAT BZ 80 at 1 g/l was prepared the same day of the test.
DILUTION WATER: was prepared in the laboratory using pure water and salts according to ISO 6341.
For one litre: 25 ml of the below solutions
1- 11.76g CaCl2,2H2O/l ultrapure water.
2- 4.93g MgSO4,7H2O/l ultrapure water.
3- 2.59g NaHCO3/l ultrapure water.
4- 0.23g KCl/l ultrapure water.
- Aeration: aerated up until oxygen saturation.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Daphnia magna.
- Source: Breeding colony was realized in the laboratory in ASTM medium, organisms were selected by sieving.
- Age at study initiation (mean and range, SD): : Less than 24 hours
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Feeding during test
- Food type: Microscopic algae Selenastrum capricornutum
- Amount:
- Frequency:

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Not specified

Test temperature:

20±1°C

pH:

Not specified

Dissolved oxygen:

>2 mg/l.

Nominal and measured concentrations:

Nominal: 6, 10, 20, 30, 50, 60, 100 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Glass tube (I=180mm, d =
- Aeration: No

- Renewal rate of test solution (frequency/flow rate): No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:was prepared in the laboratory using pure water and salts according to ISO 6341.
For one litre: 25 ml of the below solutions
1- 11.76g CaCl2,2H2O/l ultrapure water.
2- 4.93g MgSO4,7H2O/l ultrapure water.
3- 2.59g NaHCO3/l ultrapure water.
4- 0.23g KCl/l ultrapure water.
- Aeration: aerated up until oxygen saturation.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:Incubation of test flasks in darkness.



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The percentage of daphnids immobilisation after 24 and 48 hours.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

44 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 24-79 mg/l

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

61 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 38-98 mg/l)

Effect concentrations 6

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Quality criteria: Concentration of dissolved oxygen in the test vessels remained above 2mg/l at the end of the test. -Immobilisation in the control did not exceed 10% at the end of the test.

Conclusions:

The EC50 of ADAMQUAT BZ 80 to Daphnia magna at 48 hours is 44 mg/l.