5H-Cyclopenta[h]quinazoline, 6,6a,7,8,9,9a-hexahydro-7,7,8,9,9-pentamethyl-

Short-term toxicity to aquatic invertebrates

Description of key information

The acute toxicity to aquatic invertebrates was examined in a study according to OECD TG 202, and in compliance with GLP criteria (Harlan, 2014). In this study groups of Daphnia magna (4 replicates of 5), were exposed to nominal test concentrations of 1, 3.2, 10, 32 and 100% saturated solution for 48 hours under static conditions. The concentration and stability of the test item in the test preparations were verified by chemical analysis of the 10, 32 and 100% saturated solution test preparations at 0 and 48 hours. At the start of the test, measured concentrations were 0.56, 1.8, and 6.2 mg/L. No change was found at 48 hours and therefore results were based on initial measured test concentrations. Daphnia immobility was recorded after 24 and 48 hours exposure. All validity criteria were met.

Up to the test concentration of 0.56 mg/L no daphnids were immobilized, whereas at 1.8 and 6.2 mg/L (corresponding to 32% and 100% saturated) all daphnids were immobilized after the 48-hour exposure period. Based on these findings, the 48-h EC50 value was determined to be 1.0 mg/L (95% C.L.: 0.56 - 1.8 mg/L), based on initial measured concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 mg/L