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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Relative toxicities of chemicals to the earthworm Eisenia foetida
Author:
Roberts, B.L. and Dorough, H.W.
Year:
1984
Bibliographic source:
Environmental Toxicology and Chemistry 3: 67-78

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Principles of method if other than guideline:
Acute filter paper test with Eisenia fetida
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Methanol
EC Number:
200-659-6
EC Name:
Methanol
Cas Number:
67-56-1
IUPAC Name:
methanol
Details on test material:
- Name of test material (as cited in study report): Methanol
- Analytical purity: analytical grade

Sampling and analysis

Analytical monitoring:
not specified

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Method of application to filter paper (if used): Methanol was dissolved in acetone, or other suitable solvent, and 1 mL of the solution was pipetted into each vial. A stream of warm air was directed into the vial to aid in evaporation of the solvent. After the vials had been under the stream of warm air for 3 min, they were air-dried for an additional 2 h to remove all remaining solvent. Then, 1 mL of water was added to each vial to moisten the paper and one earthworm was placed into each vial.
- Controls: Control vials received only the 1 mL of acetone
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone, or other suitable solvent
- Evaporation of vehicle before use: yes; a stream of warm air was directed into the vial to aid in evaporation of the solvent

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: Bert's Bait Farm, Irvine, KY, USA
- Age at test initiation (mean and range, SD): Mature worms showing a developed clitellum
- Weight at test initiation (mean and range, SD): 370 to
450 mg


ACCLIMATION
- Acclimation conditions (same as test or not): worms were housed in Nalgene boxes filled with moist peat moss and rabbit manure, and stored at 18°C. Calcium carbonate was added as necessary to maintain soil pH above 5.5

Study design

Study type:
laboratory study
Substrate type:
filter paper
Limit test:
no
Total exposure duration:
48 h

Test conditions

Details on test conditions:
TEST SYSTEM
- Test container (material, size): Glass shell vials (22 mm x 85 mm) (Research Products International, Mt. Prospect, IL, USA) were lined with Whatman No. 1 filter paper strips (9.5 cm x 6.8 cm, surface area, 65 cm 2) and placed in cardboard scintillation vial trays. Vials were capped.
- No. of organisms per container (treatment): 1
- No. of replicates per treatment group: 10
- No. of replicates per control: 1
- a minimum of 100 earthworms was tested in order to determine the LC50

OTHER TEST CONDITIONS
- Photoperiod: dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality

VEHICLE CONTROL PERFORMED: yes


TEST CONCENTRATIONS
- Spacing factor for test concentrations: geometric series of five to seven concentrations
- Range finding study
- Test concentrations: 0.1, 1, 10, 100, 1000 µg/cm2 (nominal)
- Results used to determine the conditions for the definitive study: The approximate lethal concentration was considered to be the lowest exposure that killed 50 % or more of the earthworms.
Nominal and measured concentrations:
0.1, 1, 10, 100, 1000 µg/cm2 (nominal)
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 1 mg/cm²
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion