Dodecan-1-ol, ethoxylated

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: negetive control with only test medium
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle was used
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): The test solution prepared was soluble and homogenious with no visible particles

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water Flea
- Age at study initiation (mean and range, SD): <24 hours
- Stage and instar at study initiation: young daphnids
- Method of breeding: Ecotoxicology, Eurofins Advinus Limited
- Source: Ecotoxicology, Eurofins Advinus Limited
- Feeding during test no feeding during test


ACCLIMATION
- Acclimation conditions (same as test or not): same as test condition
- Type and amount of food: daily with unicellular green alga, Chlorella vulgaris
- Health during acclimation (any mortality observed): healthy

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

250 CaCO3/L

Test temperature:

20.0 to 20.6 °C

pH:

7.68 to 7.72

Dissolved oxygen:

6.8 to 7.3

Salinity:

not applicable

Conductivity:

0.054 µS/cm

Nominal and measured concentrations:

Nominal concentration 0.5, 1.0, 2.0, 4.0, 8.0 and 16 mL

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL glass beakers
- Type (delete if not applicable): loosely covered
- Material, size, headspace, fill volume: glass, 100ml and 250 ml
- Volume of solution: 150ml
- Aeration: no aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): no vehicle control was used

test medium parameters:
To prepare test medium every time, each stock solutions were taken in the volume ratio of 1:1:1:1 and diluted to 40 times the volume of each stock solution taken (or 10 times the total volume of all the four stock solutions taken). The test medium was aerated until the dissolved oxygen concentration equaled the air-saturation value.

The pH of the test medium used for the range finding test was 7.68 and in definitive test it was 7.70.

The hardness and total organic carbon (TOC) of reconstituted water was 250 CaCO3/L and 0.8 mg/L, respectively during definitive test.

The test medium so prepared was set aside without aeration for a minimum period of 2 days before use and was used for the treatment within 2-6 days of preparation.

Chemicals used to prepare the test medium are given in below tables

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Light intensity: 620 to 642 lux


VEHICLE CONTROL PERFORMED: NO

RANGE-FINDING STUDY
- Test concentrations: 0.01, 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: immobilisation in each replicate recorded and definitive test concentration was taken as 0.5, 1.0, 2.0, 4.0, 8.0 and 16 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

9.131 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI 2.76515 to 14.37702 mg/L

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

11.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % CI limits 8.55403 to 16.58231 mg/L

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: None
- Mortality of control: no mortality was found in control group
- Immobilisation of control: No immobilisation was found in control group
- Abnormal responses: None
- Effect concentrations exceeding solubility of substance in test medium: test chemical was found to have higher solublity in test medium than water.

Results with reference substance (positive control):

- Results with reference substance valid,yes valid potassium dichromate
- Relevant effect levels: EC50 24 hours 0.58 mg/l
- Dose-response test: 0.1, 0.2, 0.4, 0.8, 3.2 mg/L
- EC50 : 0.58 mg/L (24 hours)
-EC50: 0.2954 mg/L

Reported statistics and error estimates:

The EC50 value with 95% fiducial limits and the equation for the dose-response curve was determined by Probit analysis by the method of Finney (1978) using a validated computer program

Tables:   Range Finding Test – Test Conditions

Dissolved Oxygen Concentration and pH of Test Concentrations

Group	Treatment (mg/L)	Dissolved oxygen concentration (mg/L)		pH
		Start of test	End of test (48 Hours)	Start of test	End of test (48 Hours)
G1	Negative control	7.1	6.9	7.63	7.66
G2	0.01	7.2	7.1	7.64	7.65
G3	0.1	7.1	7.0	7.65	7.67
G4	1	6.9	6.7	7.64	7.65
G5	10	7.2	7.0	7.66	7.68
G6	100	7.3	7.1	7.68	7.69

 

Temperature and Light Intensity Measurements of Growth Chamber

Date	Temperature (°C)			Light intensity (Lux)
	Minimum	Maximum	Growth chamber display value
12 October 2020	20.3	20.4	20.5	625
13 October 2020	20.2	20.5	20.6	627
14 October 2020	20.1	20.4	20.5	631
15 October 2020	20.0	20.3	20.5	634

TABLE 1.            Range Finding Test – Results on Daphnia Immobilization

Group	Treatment (mg/L)	No. of Daphnia tested (5 per replicate)	No. of Daphnia Immobilized at				% Immobilization
			24 hours		48 hours		24 hours	48 hours
			R1	R2	R1	R2
G1	Negative control	10	0	0	0	0	0	0
G2	0.01	10	0	0	0	0	0	0
G3	0.1	10	0	0	0	0	0	0
G4	1	10	0	0	1	0	0	10
G5	10	10	2	1	4	3	30	70
G6	100	10	2	4	4	5	60	90

 Definitive Test – Test Conditions

Dissolved Oxygen Concentration and pH of Test Concentrations

Group	Treatment (mg/L)	Dissolved oxygen concentration (mg/L)		pH
		Start of test	End of test (48 Hours)	Start of test	End of test (48 Hours)
G1		Negative control		7.3		6.9		7.69		7.71
G2		0.5		7.0		6.8		7.70		7.72
G3		1.0		6.9		6.8		7.67		7.69
G4		2.0		7.2		7.1		7.71		7.72
G5		4.0		7.1		7.0		7.68		7.69
G6		8.0		7.0		6.9		7.69		7.71
G7		16.0		7.2		7.1		7.70		7.72
Mean ± SD			7.0±0.15			7.70 ± 0.02

 

Temperature and Light Intensity Measurements of Growth Chamber

Date	Temperature (°C)			Light intensity (Lux)
	Minimum	Maximum	Growth chamber display value
18 January 2021	20.2	20.4	20.6	620
19 January 2021	20.3	20.5	20.6	638
20 January 2021	20.0	20.3	20.5	642
21 January 2021	20.1	20.4	20.6	629

 

 

TABLE 3.            Definitive Test – Results on Daphnia Immobilization

Group	Treatment (mg/L)	No. of Daphnia immobilized (5 Daphnia per replicate)								% Immobilization
		24 h				48 h				24 h	48 h
		R1	R2	R3	R4	R1	R2	R3	R4
G1	Negative control	0	0	0	0	0	0	0	0	0	0
G2	0.5	0	0	0	0	0	0	0	0	0	0
G3	1.0	0	0	0	0	0	0	0	0	0	0
G4	2.0	0	1	0	0	0	1	1	0	5	10
G5	4.0	0	0	1	1	0	1	1	1	10	15
G6	8.0	1	1	1	0	1	2	1	0	15	20
G7	16.0	4	3	4	4	5	4	5	4	75	90

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Validity criteria fulfilled:

yes

Remarks:

There was no immobilization of daphnia in the control during the test period. The dissolved oxygen concentration at the end of the test was more than ≥ 3 mg/L in negative control and treatment test vessels.

Conclusions:

The median effective concentration of test chemical was evaluated to be 11.909 and 9.1313 mg/l for 24 and 48 hours of exposure respectively to daphnia magna.

Executive summary:

The acute immobilization effect of the test item was studied on Daphnia magna for 48 hours. the study was conducted by following the OECD 202 test guidelines, by observing the immobilisation caused during exposure period of 24 and 48 hours. Test system was daphnia magna with age less than 24 hours. During acclimitisation, test medium in which the parent daphnia were maintained was changed at least twice a week and they were fed with the unicellular green alga,Chlorella vulgaris. The medium used for the test was reconstituted water as described in the OECD test guideline 202 (OECD, 2004). The conductivity of the deionized water used for the preparation of the test medium was 0.054 µS/cm. The hardness and total organic carbon (TOC) of reconstituted water was 250 CaCO₃/L and 0.8 mg/L. The test medium so prepared was set aside without aeration for a minimum period of 2 days before use and was used for the treatment within 2-6 days of preparation. One day prior to the start of the treatment, about 20 adults were incubated in approximately 2 L of reconstituted water. The following day, the young ones hatched which were less than 24 hour old were collected and used in the tests. During range finding study test organism was taken and exposed to the concentrations ranging form 0.01, 0.1,1, 10 and 100 mg/L along with control. There was no Immobilization of daphnia observed at the tested concentrations of 0.01, 0.1 and 1 at 24 hours exposure and there was no Immobilization of daphnia observed at the tested concentrations of 0.01 and 0.1 at 48 hours exposure.however, There was 30 and 60 % immobility of the daphnia observed at 24 hour at the tested concentrations of 10 and 100 mg/L, respectively and 10, 70 and 90 % immobility of the daphnia observed at 48 hour at the tested concentrations of 1, 10 and 100 mg/L, respectively. Based on the outcomes In definitive test, Daphnia magna less than 24 hours old were exposed to the nominal concentrations of 0.5, 1.0, 2.0, 4.0, 8.0 and 16 mg/L. The number of daphnia immobilized was recorded at 24 and 48 hours exposure. There was no immobilization of daphnia in the negative control and at the tested concentrations of 0.5 and 1.0 mg/L at 24 and 48 hours of exposure. The immobilization of daphniawas 5, 10, 15 and 75 % at 24 h and 10, 15, 20 and 90 % at 48 h exposure at 2.0, 4.0, 8.0 and 16 mg/L, respectively. The stability test results concluded that the test item was stable in the test medium at 48 h at nominal concentrations.The active ingredient concentration analysis in all test concentrations showed that the mean percent recovery with nominal concentration was in the range of 94.31 to 105.59 %(RSD was 2.52 to 7.97%)at the start and 93.98 to 116.77 %(RSD was 3.48 to 19.83 %)at the end of the test (48 hour) indicating that the results were within the acceptable limit (80 to 120 % of the nominal concentration with an RSD of<20%). All the validity criteria was maintained within the acceptibility domain, such as, there was no mortality in test controls and the dissolved oxygen concentration was > 3mg/l. To validate the study reference study was also conducted using potassium dichromate for 48 hours and EC50 was recorded for 24 and 48 hours.

The EC50 value for test chemical at 24 hours and 48 hours was 11.9099 mg/L and 9.1313 mg/L with fiducial limits at 95 per cent ranging from 8.55403 to 16.58231 and

2.76515 to 14.3770 respectively based on nominal concentrations. On the basis of observation made during the test, the LOEC (Low Observed Effect Concentration) was 2.0 mg/L based on nominal concentrations. On the basis of observation made during the test, the NOEC (No Observed Effect Concentration) was 1.0 mg/L based on nominal concentrations.                                       

Description of key information

The acute immobilization effect of the test item was studied onDaphnia magnafor 48 hours. the study was conducted by following the OECD 202 test guidelines, by observing the immobilisation caused during exposure period of 24 and 48 hours. Test system was daphnia magna with age less than 24 hours. During acclimitisation, test medium in which the parent daphnia were maintained was changed at least twice a week and they were fed with the unicellular green alga,Chlorella vulgaris. The medium used for the test was reconstituted water as described in the OECD test guideline 202 (OECD, 2004). The conductivity of the deionized water used for the preparation of the test medium was 0.054 µS/cm. The hardness and total organic carbon (TOC) of reconstituted water was 250 CaCO₃/L and 0.8 mg/L. The test medium so prepared was set aside without aeration for a minimum period of 2 days before use and was used for the treatment within 2-6 days of preparation. One day prior to the start of the treatment, about 20 adults were incubated in approximately 2 L of reconstituted water. The following day, the young ones hatched which were less than 24 hour old were collected and used in the tests. During range finding study test organism was taken and exposed to the concentrations ranging form 0.01, 0.1,1, 10 and 100 mg/L along with control. There was no Immobilization of daphnia observed at the tested concentrations of 0.01, 0.1 and 1 at 24 hours exposure and there was no Immobilization of daphnia observed at the tested concentrations of 0.01 and 0.1 at 48 hours exposure.however, There was 30 and 60 % immobility of the daphnia observed at 24 hour at the tested concentrations of 10 and 100 mg/L, respectively and 10, 70 and 90 % immobility of the daphnia observed at 48 hour at the tested concentrations of 1, 10 and 100 mg/L, respectively. Based on the outcomes In definitive test,Daphnia magnaless than 24 hours old were exposed to the nominal concentrations of 0.5, 1.0, 2.0, 4.0, 8.0 and 16 mg/L. The number of daphnia immobilized was recorded at 24 and 48 hours exposure. There was no immobilization of daphnia in the negative control and at the tested concentrations of 0.5 and 1.0 mg/L at 24 and 48 hours of exposure. The immobilization of daphniawas 5, 10, 15 and 75 % at 24 h and 10, 15, 20 and 90 % at 48 h exposure at 2.0, 4.0, 8.0 and 16 mg/L, respectively. The stability test results concluded that the test item was stable in the test medium at 48 h at nominal concentrations.The active ingredient concentration analysis in all test concentrations showed that the mean percent recovery with nominal concentration was in the range of 94.31 to 105.59 %(RSD was 2.52 to 7.97%)at the start and 93.98 to 116.77 %(RSD was 3.48 to 19.83 %)at the end of the test (48 hour) indicating that the results were within the acceptable limit (80 to 120 % of the nominal concentration with an RSD of<20%). All the validity criteria was maintained within the acceptibility domain, such as, there was no mortality in test controls and the dissolved oxygen concentration was > 3mg/l. To validate the study reference study was also conducted using potassium dichromate for 48 hours and EC50 was recorded for 24 and 48 hours.

The EC50 value for test chemical at 24 hours and 48 hours was 11.9099 mg/L and 9.1313 mg/L with fiducial limits at 95 per cent ranging from 8.55403 to 16.58231 and

2.76515 to 14.3770 respectively based on nominal concentrations. On the basis of observation made during the test, the LOEC (Low Observed Effect Concentration) was 2.0 mg/L based on nominal concentrations. On the basis of observation made during the test, the NOEC (No Observed Effect Concentration) was 1.0 mg/L based on nominal concentrations.                                       

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

9.131 mg/L

Additional information

Short term toxicity to Aquatic invertebrate:

The acute immobilization effect of the test item was studied onDaphnia magnafor 48 hours. the study was conducted by following the OECD 202 test guidelines, by observing the immobilisation caused during exposure period of 24 and 48 hours. Test system was daphnia magna with age less than 24 hours. During acclimitisation, test medium in which the parent daphnia were maintained was changed at least twice a week and they were fed with the unicellular green alga,Chlorella vulgaris. The medium used for the test was reconstituted water as described in the OECD test guideline 202 (OECD, 2004). The conductivity of the deionized water used for the preparation of the test medium was 0.054 µS/cm. The hardness and total organic carbon (TOC) of reconstituted water was 250 CaCO₃/L and 0.8 mg/L. The test medium so prepared was set aside without aeration for a minimum period of 2 days before use and was used for the treatment within 2-6 days of preparation. One day prior to the start of the treatment, about 20 adults were incubated in approximately 2 L of reconstituted water. The following day, the young ones hatched which were less than 24 hour old were collected and used in the tests. During range finding study test organism was taken and exposed to the concentrations ranging form 0.01, 0.1,1, 10 and 100 mg/L along with control. There was no Immobilization of daphnia observed at the tested concentrations of 0.01, 0.1 and 1 at 24 hours exposure and there was no Immobilization of daphnia observed at the tested concentrations of 0.01 and 0.1 at 48 hours exposure.however, There was 30 and 60 % immobility of the daphnia observed at 24 hour at the tested concentrations of 10 and 100 mg/L, respectively and 10, 70 and 90 % immobility of the daphnia observed at 48 hour at the tested concentrations of 1, 10 and 100 mg/L, respectively. Based on the outcomes In definitive test,Daphnia magnaless than 24 hours old were exposed to the nominal concentrations of 0.5, 1.0, 2.0, 4.0, 8.0 and 16 mg/L. The number of daphnia immobilized was recorded at 24 and 48 hours exposure. There was no immobilization of daphnia in the negative control and at the tested concentrations of 0.5 and 1.0 mg/L at 24 and 48 hours of exposure. The immobilization of daphniawas 5, 10, 15 and 75 % at 24 h and 10, 15, 20 and 90 % at 48 h exposure at 2.0, 4.0, 8.0 and 16 mg/L, respectively. The stability test results concluded that the test item was stable in the test medium at 48 h at nominal concentrations.The active ingredient concentration analysis in all test concentrations showed that the mean percent recovery with nominal concentration was in the range of 94.31 to 105.59 %(RSD was 2.52 to 7.97%)at the start and 93.98 to 116.77 %(RSD was 3.48 to 19.83 %)at the end of the test (48 hour) indicating that the results were within the acceptable limit (80 to 120 % of the nominal concentration with an RSD of<20%). All the validity criteria was maintained within the acceptibility domain, such as, there was no mortality in test controls and the dissolved oxygen concentration was > 3mg/l. To validate the study reference study was also conducted using potassium dichromate for 48 hours and EC50 was recorded for 24 and 48 hours.

The EC50 value for test chemical at 24 hours and 48 hours was 11.9099 mg/L and 9.1313 mg/L with fiducial limits at 95 per cent ranging from 8.55403 to 16.58231 and

2.76515 to 14.3770 respectively based on nominal concentrations. On the basis of observation made during the test, the LOEC (Low Observed Effect Concentration) was 2.0 mg/L based on nominal concentrations. On the basis of observation made during the test, the NOEC (No Observed Effect Concentration) was 1.0 mg/L based on nominal concentrations.                                    

In an experimental study (Study report, 2019), an acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202 “Daphnia sp., Acute Immobilization Test”. The stock solution was prepared by dissolving 100mg of test chemical in 1L of ADaM’s media, From which further test concentrations of 0, 1, 2, 4, 8 and 16 mg/L were prepared. The highest and lowest exposed test chemical concentrations were verified analytically at day 0 and day 2 which has been satisfactorily maintained within ± 20 % of the nominal initial concentration throughout the test. Study was performed using 10 daphnids in a static fresh water system. Total 10 Daphnids were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±2°C, pH 7.3, hardness of water 150.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. One control containing solubilizing agent was also run simultaneously during the study. The animals were exposed to medium (i.e.a beaker containing only medium) and the tested chemical during 48 hour. The daphnids were moving slowly as compared to control. On the basis of this, the median effect concentration (EC50 (48 h)) value was determined to be 5.2 mg/L (nominal concentration). Thus, based on the EC50 value, test chemical can be considered as toxic to aquatic invertebrates and can be expected to be classified in ‘Aq. chronic category 2’. However, test chemical is readily biodegradable in water, chemical is considered as non-toxic to aquatic invertebrates and thus, considered to be ‘not classified’ as per the CLP classification criteria.

 

In a supporting weight of evidence study, short term toxicity to aq. Invertebrate study was conducted for 96 hrs for assessing the effect of test chemical (C. Pantani et. al., 1997 and secondary source, 2019). The study was performed under static conditions at 8±0.5°C. Gammarus italicus and Echinogammarus tibaldii collected near the spring river Vera (L’Aquila, Italy) were used as a test organism. Male test organisms were acclimatized for about 3 days in cool reconstituted water, which received artificial oxygenation. During the acclimation period, test organism was fed with dry poplar leaves previously soaked in spring water in order to enrich them with fungi and bacteria. Feeding was stop 24 hrs before the experiment. The reconstituted water has hardness 240 mg/l as CaCO3, alkalinity 55 mg/l as CaCO3 and pH 7.9±0.5, respectively. Six concentration of test chemical was taken based on the range finding study with the same logarithmic interval. Study was performed in 1 l glass jar containing 250 ml of the precooled aeration solution for 96 hrs. No aeration was provided in the test vessels. Test organisms was not fed during the test. All tests (test vessels including the control) were performed in 2 replicates using 20 test organisms of Echinogammarus tibaldii and less no. of Gammarus italicusi / replicate. Test organisms which showed no movements of the pleopodes when gently prodded with a spatula after 96 hr were considered as dead. Data was analysed through probits and the LC50 was expressed as nominal concentrations. On the basis of effect on mobility of the test organism G. italicus and E. tibaldii, the 96 hr LC50 was determined to be 7.6 mg/l (95% C. I. – 7.3 to 8.1 mg/l) and 3.6 mg/l (3.4 to 3.8 mg/l) (nominal concentration), respectively. Thus, test chemical can be considered as toxic to aquatic invertebrates. Since the test chemical is readily biodegradable in water, chemical is considered to be non-toxic to aquatic invertebrates.

 

For the test chemical, short term toxicity to aq. Invertebrate study was conducted for 24 hrs for assessing the effect of test chemical (C. Pantani et. al., 1990). The study was performed under static conditions. Gammarus italicus collected near the spring river Vera (L’Aquila, Italy) was used as a test organism. Male test organisms were acclimatized for about 3 days in artificial water at a constant temperature of 8°C, which received artificial oxygenation. During the acclimation period, test organism was fed with dry poplar leaves previously soaked in spring water in order to enrich them with fungi and bacteria. Feeding was stop 24 hrs before the experiment. Oxygenation was not provided in the test vessels. In addition to this, test organism was not fed during the study. Test fishes were exposed to the test chemical concentration at 8°C under test conditions which includes hardness 240 mg/l as CaCO3, alkalinity 55 mg/l as CaCO3 and pH 8.1, respectively. Test organisms which showed no movements of the pleopodes when gently prodded with a spatula after 24 hr were considered as dead. Data was analysed through probit with the help of a program in BASIC and the LC50 was expressed in mg/l. On the basis of effect on mobility of the test organism Gammarus italicus,the 48 hr LC50 was determined to be 9.45 mg/l (95% C. I. – 8.83 to 10.18 mg/l) (nominal concentration).Thus, test chemical can be considered as toxic to aquatic invertebrates. Since the test chemical is readily biodegradable in water, chemical is considered as non-toxic to aquatic invertebrates.

 

Another short term toxicity to aq. Invertebrate study was conducted for 48 hrs for assessing the effect of test chemical (authoritative database, 2018 and secondary source, 2019). This test was performed using Daphnia magna (water flea) under static conditions at 20°C.No analytical monitoring was performed of exposed test chemical concentrations. On the basis of effect on themortality of the test organism, the 48 hr LC50 value was determined to be 6.46 mg/l (95% C. I. – 4.78 to 7.58 mg/l) (nominal concentration). Based on this value, test chemical can be considered as toxic to aquatic invertebrates. Since the test chemical is readily biodegradable in water, chemical is non-toxic to aquatic invertebrates and is considered to be ‘not classified’ as per the CLP classification criteria.

 

On the basis of the above results, it can be concluded that the test chemical can be considered as toxic to aquatic invertebrates. However, test chemical is readily biodegradable in water, chemical is considered as non-toxic to aquatic invertebrates and thus, considered to be ‘not classified’ as per the CLP classification criteria.