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EC number: 227-815-6
CAS number: 5989-54-8
Following 30 minutes of exposure, LC50 in
mouse was determined to be higher than the highest tested dose, i.e.
2421 ppm, because no treatment-related death was observed at any of the
treatment doses. Based on the conversion factor , as 1 ppm corresponds
to 5.56 mg/m3 for d-limonene. LC50 is
determined to be greater than 8890.44 mg/m3 following 30 minutes of
exposure and 1680 mg/m3 (1.68 mg/L) following 4 hours of exposure, based
on Haber’s law (Cn x t = k), where n = 1.
Table 1: RD50 and RD0 values at different
Range of concentrationb(ppm)
Slopec(%/ log concentration)
61.9 ± 6.3
1467 (902 -2387)
232 (125 -431)
53.1 ± 4.0
1734 (1205 -2495)
202 (125 -326)
46.3 ± 4.0
1918 (1274 -2887)
159 (90 -281)
53.8 ± 3.1
199 (129 -307)
of mice used for construction of the regression line.
of concentration used for construction of the regression line.
± is the SD value.
95% confidence intervals are given in brackets.
In an acute inhalation toxicity study
performed similarly to OECD Guideline 403, groups (4/dose) of male
BALB/cA mice were exposed (head only) to vapors of S-(-)-limonene at
concentration range of 316 -2421 ppm (measured) for 30 min. Animals were
observed for mortality and signs of sensory irritation, airflow
limitation and pulmonary irritation during the exposure period.
One mouse died at 1788 and one at 2421 ppm
S-(-)-limonene concentrations, after 2 and 4 min, respectively. There
was no concentration or time -dependent effect of death and none of the
deaths is considered to be exposure related.
A dose-dependent decrease in respiratory
rate and increase in time of break indicated the sensory irritant effect
The RD50 values at 0-10, 11-20, 21-30 or
0-30 min were estimated to be 1467 (902 -2387), 1734 (1205 -2495), 1918
(1274 -2887) or 1715 (1168 -2519), respectively.
Under the test conditions, as no death was
considered to be treatment-related, the acute inhalation LC50 (30 min)
value was estimated to be greater than 2421 ppm in male mice. LC50 (4 h)
was calculated to be > 1.68 mg/L.
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