acetalization product between glucose and C16-18(even numbered)- alcohol

Toxicity to soil microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 August 2014 to 23 September 2014

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)

Version / remarks:

OECD 216 version 2000

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch No. T22335
- Purity test date: 06 June 2005

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored at room temperature in the dark

Analytical monitoring:

no

Vehicle:

no

Test organisms (inoculum):

soil

Total exposure duration:

28 d

Test temperature:

The soil samples were incubated in 500 mL glass duran bottles sealed and maintained in a temperature controlled room at temperature of between 20 and 22 °C in darkness throughout the test.

Moisture:

The moisture content of each control and test vessel was adjusted to 45% of the Water Holding Capacity (± 5%) of the soil on Days 7, 14, 21 and 28.

Organic carbon content (% dry weight):

0.67

Nitrogen content (% dry weight):

2.9

Details on test conditions:

TEST SYSTEM
- Testing facility: Harlan Laboratories
- Test container (type, material, size): 500 mL glass duran bottles sealed with polyurethane foam stoppers
- Amount of soil: 0.5 kg of soil
- No. of replicates per concentration: 3 replicate
- No. of replicates per control: 3 replicate
- No. of replicates per vehicle control: 3 replicate

SOIL INCUBATION
- Method: series of individual and equally sized subsamples of each treated and untreated soil.

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- History of site: The sampling site had not been treated with crop protection products or organic fertilizer for at least 3 years prior to sampling.
- Vegetation cover: The site was uncultivated
- Treatments with pesticides or fertilizers: None traitement was done.
- Depth of sampling: 30 cm
- % sand: 57.6%
- pH (in water): 6.4
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): 1,46 mg/100 g is 14,6 mg/kg dw
- Maximum water holding capacity (in % dry weigth): 35.6 g/100 g ± 1.7. The moisture content of the soil, expressed as a percentage of th dry weight, was determined to be 5%.
- Storage (condition, duration): The soil was stored at a temperature of approximately 4°C for 22 days prior to use.Prior to the start the test, an amount of soil (appproximately 8 kg ) was removed and stored at approximately 21°C for 8 days and shielded from light.
- Initial microbial biomass as % of total organic C: The microbial biomass of the soilwas shown to be 35.89 mg C/100 g .This was equivalent to 5.36% of the total soil carbon content.

VEHICLE CONTROL PERFORMED: No

Nominal and measured concentrations:

Aliquots (50 mL) of the 100, 320, 1000 and 3200 mg/L stock dispersions were sprinkled separately over the surface of 0.5 kg of soil and throughly mixed.

Reference substance (positive control):

no

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

ca. 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Key result

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Conclusions:

The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 of greater than 1000 mg/kg.

The test item showed no significant effect on the nitrogen transformation activity of soil microorganisms at a test concentration of 1000 mg/kg soil dry weight over a 28-Day period and therefore can be considered to have no long-term effect on nitrogen transformation in soil.

Therefore the No Observed Effect Concentration (NOEC) was 1000 mg/Kg

Executive summary:

A study was performed to asses the long-term effect of the test item, after a single exposure, on the nitrogen transformation activity of soil microorganisms.

Soil microorganisms were exposed to the test item at concentrations of 10,32,100,320 and 1000 mg/kg for 28 days at temperatures between 20 and 22 °C in the dark. Lucerne-green-grass meal was added to the soil to act as a respiratory substrate.

The inhibitory effect of the test item on nitrogen transformation was assessed by the determination of nitrate concentration in the soil samples on Days 0 and 28 and compared to data obtained from control soil samples

The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 of greater than 1000 mg/kg.

The test item showed no significant effect on the nitrogen transformation activity of soil microorganisms at a test concentration of 1000 mg/kg soil dry weight over a 28-Day period and therefore can be considered to have no long-term effect on nitrogen transformation in soil.

Therefore the No Observed Effect Concentration (NOEC) was 1000 mg/Kg

Description of key information

A study was performed to assess the long-term effect of the test item, after a single exposure, on the nitrogen transformation activity of soil microorganisms.

Soil microorganisms were exposed to the test item at concentrations of 10, 32,100, 320 and 1000 mg/kg for 28 days at temperatures between 20 and 22 °C in the dark. Lucerne-green-grass meal was added to the soil to act as a respiratory substrate.

The inhibitory effect of the test item on nitrogen transformation was assessed by the determination of nitrate concentration in the soil samples on Days 0 and 28 and compared to data obtained from control soil samples.

The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 of greater than 1000 mg/kg.

The test item showed no significant effect on the nitrogen transformation activity of soil microorganisms at a test concentration of 1000 mg/kg soil dry weight over a 28-Day period and therefore can be considered to have no long-term effect on nitrogen transformation in soil.

Therefore the No Observed Effect Concentration (NOEC) was 1000 mg/Kg

Key value for chemical safety assessment

Long-term EC10 or NOEC for soil microorganisms:

1 000 mg/kg soil dw