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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed according to OECD guideline 420 and in compliance to GLP.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005
Reference Type:
other: Published secondary source
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 420 (Acute Oral Toxicity - Fixed Dose Method)
GLP compliance:
yes
Test type:
fixed dose procedure
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-[[4-[(2-hydroxyethyl)amino]-3-nitrophenyl]imino]bisethanol
EC Number:
251-410-3
EC Name:
2,2'-[[4-[(2-hydroxyethyl)amino]-3-nitrophenyl]imino]bisethanol
Cas Number:
33229-34-4
Molecular formula:
C12H19N3O5
IUPAC Name:
2-({4-[bis(2-hydroxyethyl)amino]-2-nitrophenyl}amino)ethan-1-ol
Details on test material:
Test item : 2,2'-[[4-[(2-hydroxyethyl)amino]-3-nitrophenyl]imino]bisethanol
EC number : 251-410-3
Batch number : 31394
Content : 99.5%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: 0.5% suspension of carboxymethylcellulose in purified water
Doses:
2000 mg/kg bw
No. of animals per sex per dose:
1(sighting), 4(main experiment)
Control animals:
no

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
95% CL:
ca. 0
Remarks on result:
other: confidence level cannot be established therefore expressed as circa 0
Mortality:
No mortality was recorded.
Clinical signs:
other: Hypoactivity, piloerection and dyspnea were observed in all females within 4 hours of treatment. Blue spots were noted on the tail and/or on the fur of all females from day 1 up to day 8.
Gross pathology:
At necropsy, no apparent abnormalities were observed.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Under the experimental conditions, the maximal non-lethal dose of the test item was 2000 mg/kg by oral route in rats. In accordance with Directive 67/548 and Regulation (EC) No 1272/2008 the substance is not classified.
Executive summary:

The acute oral toxicity of the substance was assessed according to OECD guideline 420 (acute oral toxicity - fixed dose method) in compliance to GLP. Under the experimental conditions, the maximal non-lethal dose of the test item was 2000 mg/kg by oral route in rats. In accordance with Directive 67/548 and Regulation (EC) No 1272/2008 the substance is not classified.