p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline202 with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

EC Number:

221-453-2

EC Name:

p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

Cas Number:

3101-60-8

Molecular formula:

C13H18O2

IUPAC Name:

2-[(4-tert-butylphenoxy)methyl]oxirane

Constituent 2

Reference substance name:

p-tert-butylphenyl-1-(2,3)-epoxypropyl ether

IUPAC Name:

p-tert-butylphenyl-1-(2,3)-epoxypropyl ether

Constituent 3

Reference substance name:

Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-

IUPAC Name:

Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-

Test material form:

other: Liquid at room temperature.

Details on test material:

As per IUCLID5 Sections 1.1. 1.2. and 4.1.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

Stock solutions of the test substance were prepared at concentrations of 12.5, 25, 50 and 100 mg/L by adding the required nominal amount of test substance in the diluen (laboratory grade water), and mixing the solution for approximately 1 hour.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnia magna for the study were cultured in the STILLMEADOW, Inc. culture laboratory and were no greater than 24 hours old at dosing. The test organisms were maintained under a 16 hours ligh/l8 hours dark cycle with dissolved oxygen concentration of 60-105% saturation at dosing and a temperature of 21°C. Daphnids were not fed during the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None.

Test conditions

Hardness:

total hardness of 205 mg/L CaCO3

Test temperature:

21°C

pH:

7.8-8.1

Dissolved oxygen:

95-100% saturation.

Salinity:

Freshwater

Nominal and measured concentrations:

0, 6.25, 12.5, 25, 50 and 100 mg/L nominal.

Details on test conditions:

Treatment was conducted in 250-mL glass beakers filled with 200 mL of the appropriate solution made up in moderately hard synthetic freshwater at 21°C. Each test concentration or untreated control consisted of three replicates of ten daphnids. At 24 and 48 hours following dosing, each beaker was examined for immobility, and the number of mobile daphnids was recorded.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 67.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

MEAN RELATIVE MOBILITY
_______________________________________________________________________________
Concentration: 0 6.25 12.5 25 50 100 mg/L
Percent 100 100 96.7 53.3 40.0 56.7

Results with reference substance (positive control):

Not applicable.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Mean untreated control mobility was 93.3%.

Conclusions:

The 48 hr EC50 based on mobility was 67.9 mg/L under the conditions of the study.

Executive summary:

The test substance, p-tert-butylphenyl-1 -(2,3)-epoxypropyl ether, was accessed for acute toxicity to Daphnia in an O.E.C.D. test guideline 202 study. The 48 hr EC50 for the test substance based on mobility was 67.9 mg/L under the conditions of the study.