Registration Dossier
Registration Dossier
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EC number: 279-211-7 | CAS number: 79665-24-0
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2011-12-16 to 2011-12-22
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study conducted in compliance with GLP regulations. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 1 mg/l was freshly prepared with dilution water and stirred for 48 h (1100rpm). Undissolved particles were removed by membrane filtration (membrane filter 0.45 µm, RC, MACHEREY-NAGEL). Centrifugation for 20 min at 10000 rpm was found to be insufficient.
- Eluate: Dilution water
- Differential loading: None, limit test item concentration
- Controls: Six replicates without test item were tested under the same test conditions as the test replicates. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus Chodat
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted to 35 - 70 µE x m-2 x s-1 for 24 h per day. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- Dilution water: 0.24 mmol Ca+mg/L.
- Test temperature:
- 22.5 - 24.0 °C, mean value: 23.3 °C
- pH:
- Nominal concentration pH-value
[mg/L] Start; 0 hours End; 72 hours
1.00 7.89 7.94
Control 7.90 7.97 - Dissolved oxygen:
- Not measured
- Nominal and measured concentrations:
- Nominal test concentrations: control and 1 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type: open, cotton wool plugs
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through: None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: Nominal: approximately 2 - 5 x 103 cells/mL, Actual: mean 4910 cells/mL
- Control end cells density: Mean 634285 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water, composition acc. to the guideline
- Hardness: 0.24 mmol Ca+Mg/L
- pH-value: 8.1 +/- 0.2
- Culture medium: Nutrient Medium Z acc. to LÜTTGE et al. (1994)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 60 - 120 µE x m-2 x s-1, mean value: 69.2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] fluorimeter, Cell density and self fluorescence was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal.
TEST CONCENTRATIONS
- Range finding study: No
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
EC50 Values of the Reference Item
based on nominal concentrations (mg/L), (0-72 hours)
Current Study Valid Range (average ± 3 x SD)
Rate-related inhibition
ErC50 0.488 0.699 ± 0.471
95 % confidence interval 0.466 – 0.512
Yield inhibition
EyC50 0.291 0.318 ± 0.147
95 % confidence interval 0.274 – 0.328 - Reported statistics and error estimates:
- NOEC/LOEC was determined by calculation of statistical significance of growth rates and yield. As standard One Way Analysis of Variance (ANOVA) and DUNNETT’s Test were used for NOEC/LOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. The SHAPIRO-WILK-Test was used to test for normally distributed populations. The Levene median test was used for equal variance. P-values for both Normality and Equal Variance test were 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute toxic effects occured within the range of solubility. The test substance is with high probability acutely not harmful to aquatic algae.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- 2011-12-16 to 2011-12-22
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study conducted in compliance with GLP regulations. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
- Justification for type of information:
- See read across jusitification document in chapter 13
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 1 mg/l was freshly prepared with dilution water and stirred for 48 h (1100rpm). Undissolved particles were removed by membrane filtration (membrane filter 0.45 µm, RC, MACHEREY-NAGEL). Centrifugation for 20 min at 10000 rpm was found to be insufficient.
- Eluate: Dilution water
- Differential loading: None, limit test item concentration
- Controls: Six replicates without test item were tested under the same test conditions as the test replicates. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus Chodat
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted to 35 - 70 µE x m-2 x s-1 for 24 h per day. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- Dilution water: 0.24 mmol Ca+mg/L.
- Test temperature:
- 22.5 - 24.0 °C, mean value: 23.3 °C
- pH:
- Nominal concentration pH-value
[mg/L] Start; 0 hours End; 72 hours
1.00 7.89 7.94
Control 7.90 7.97 - Dissolved oxygen:
- Not measured
- Nominal and measured concentrations:
- Nominal test concentrations: control and 1 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type: open, cotton wool plugs
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through: None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: Nominal: approximately 2 - 5 x 103 cells/mL, Actual: mean 4910 cells/mL
- Control end cells density: Mean 634285 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water, composition acc. to the guideline
- Hardness: 0.24 mmol Ca+Mg/L
- pH-value: 8.1 +/- 0.2
- Culture medium: Nutrient Medium Z acc. to LÜTTGE et al. (1994)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 60 - 120 µE x m-2 x s-1, mean value: 69.2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] fluorimeter, Cell density and self fluorescence was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal.
TEST CONCENTRATIONS
- Range finding study: No
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
EC50 Values of the Reference Item
based on nominal concentrations (mg/L), (0-72 hours)
Current Study Valid Range (average ± 3 x SD)
Rate-related inhibition
ErC50 0.488 0.699 ± 0.471
95 % confidence interval 0.466 – 0.512
Yield inhibition
EyC50 0.291 0.318 ± 0.147
95 % confidence interval 0.274 – 0.328 - Reported statistics and error estimates:
- NOEC/LOEC was determined by calculation of statistical significance of growth rates and yield. As standard One Way Analysis of Variance (ANOVA) and DUNNETT’s Test were used for NOEC/LOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. The SHAPIRO-WILK-Test was used to test for normally distributed populations. The Levene median test was used for equal variance. P-values for both Normality and Equal Variance test were 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute toxic effects occured within the range of solubility. The test substance is with high probability acutely not harmful to aquatic algae.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- 06.06.2012 - 06.07.2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study conducted in compliance with GLP regulations. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
- Justification for type of information:
- See read across justifiction document in chapter 13
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- - The test item was used in one concentration 10 mg/L.
- The basic mixture of 10 mglL was prepared by weighing 10 mg (analytical balance SBC22, Scaltec) of the test item into a glass flask and mixing with 1 L of the test medium.
- The concentration 10 mg/L was stirred over 15 min at 40°C (in the ultrasonic bath with sonication), then heated to 70°C over 2 h (stirring) in
the incubator.
- Then the suspension was stirred at 20°C over 72 h on mechanic shaker [SOPM//S7].
- Next the suspension was filtered: over a 0.45 µm membrane disc four times, over a 0.20 µm membrane disc once and over a 0.025 µm membrane disc twice.
- The preparation of the test solution was made as a WAF (WaterAccommodated Fractions). - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneiella subcapitata (Reinsch) Korshikov (syn. Selenastrum capicomutum Prinz)
- Strain: SAG 61.81
- Source (laboratory, culture collection): lnstitute of Industrial Organic Chemistry Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology, stock from The Algae Collection of G6ttingen University, Germany.
- Age of inoculum (at test initiation):
- Method of cultivation: in 250 mL Erlenmeyer flasks and incubated in 21 - 24 °C in constant illumination - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 22.6 - 23.0 °C
- pH:
- 7.50 - 8.85
- Nominal and measured concentrations:
- Nominal test concentrations: control and 10 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks
- Type: closed with air permeable stoppers
- Size, fill volume: 250 ml, 100ml
- Renewal rate of test solution: twice a week
- Initial cells density: 1 x 10*4 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- AAP medium was prepared on the basis of deionised water (filtering system SolPureT TSOPA //71]) by adding stock solutions of reagentgrade
chemicals [SOPM//18]
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: AAP medium
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: constant illumination
- Light intensity and quality: 6780 - 7850 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometric measurements after 24, 48 and 72 h of exposure - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.097 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Details on results:
- Recovery of the test item was 94.27%.
- Results with reference substance (positive control):
- The ErC50 (72h) of the control substance 3,5 dichlorophenol was 2.09 mg/I.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute toxic effects occured within the range of solubility. The test substance is with high probability acutely not harmful to aquatic algae.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 06.06.2012 - 06.07.2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study conducted in compliance with GLP regulations. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- - The test item was used in one concentration 10 mg/L.
- The basic mixture of 10 mglL was prepared by weighing 10 mg (analytical balance SBC22, Scaltec) of the test item into a glass flask and mixing with 1 L of the test medium.
- The concentration 10 mg/L was stirred over 15 min at 40°C (in the ultrasonic bath with sonication), then heated to 70°C over 2 h (stirring) in
the incubator.
- Then the suspension was stirred at 20°C over 72 h on mechanic shaker [SOPM//S7].
- Next the suspension was filtered: over a 0.45 µm membrane disc four times, over a 0.20 µm membrane disc once and over a 0.025 µm membrane disc twice.
- The preparation of the test solution was made as a WAF (WaterAccommodated Fractions). - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneiella subcapitata (Reinsch) Korshikov (syn. Selenastrum capicomutum Prinz)
- Strain: SAG 61.81
- Source (laboratory, culture collection): lnstitute of Industrial Organic Chemistry Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology, stock from The Algae Collection of G6ttingen University, Germany.
- Age of inoculum (at test initiation):
- Method of cultivation: in 250 mL Erlenmeyer flasks and incubated in 21 - 24 °C in constant illumination - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 22.6 - 23.0 °C
- pH:
- 7.50 - 8.85
- Nominal and measured concentrations:
- Nominal test concentrations: control and 10 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks
- Type: closed with air permeable stoppers
- Size, fill volume: 250 ml, 100ml
- Renewal rate of test solution: twice a week
- Initial cells density: 1 x 10*4 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- AAP medium was prepared on the basis of deionised water (filtering system SolPureT TSOPA //71]) by adding stock solutions of reagentgrade
chemicals [SOPM//18]
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: AAP medium
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: constant illumination
- Light intensity and quality: 6780 - 7850 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometric measurements after 24, 48 and 72 h of exposure - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.097 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Details on results:
- Recovery of the test item was 94.27%.
- Results with reference substance (positive control):
- The ErC50 (72h) of the control substance 3,5 dichlorophenol was 2.09 mg/I.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute toxic effects occured within the range of solubility. The test substance is with high probability acutely not harmful to aquatic algae.
Referenceopen allclose all
NOEC, LOEC, EC - values of PV-Echtbraun RL (0-72 hours)
based on nominal concentration [mg/L]
Rate-related Inhibition |
|
NOEC |
1.00 |
LOEC |
> 1.00 |
ErC10 |
> 1.00 |
ErC20 |
> 1.00 |
ErC50 |
> 1.00 |
Inhibition of Yield |
|
NOEC |
1.00 |
LOEC |
> 1.00 |
EyC10 |
> 1.00 |
EyC20 |
> 1.00 |
EyC50 |
> 1.00 |
Cell Densities
|
Nominal concentration |
Replicate |
Cell density [cells/mL] |
||||||
[mg/L] |
No. |
0 hours |
24 hours |
48 hours |
72 hours |
|
|||
1.00 |
1 |
4910 |
29017 |
165338 |
711305 |
|
|||
2 |
4910 |
21642 |
135977 |
771998 |
|
||||
3 |
4910 |
22620 |
135753 |
676405 |
|
||||
4 |
4910 |
26607 |
173530 |
760929 |
|
||||
5 |
4910 |
27315 |
163950 |
683717 |
|
||||
6 |
4910 |
24681 |
134846 |
697494 |
|
||||
Mean |
4910 |
25314 |
151566 |
716975 |
|
||||
Control |
1 |
4910 |
24999 |
142808 |
651898 |
|
|||
2 |
4910 |
23784 |
149659 |
608401 |
|
||||
3 |
4910 |
22508 |
146782 |
693872 |
|
||||
4 |
4910 |
22454 |
139897 |
566799 |
|
||||
5 |
4910 |
25470 |
140194 |
624886 |
|
||||
6 |
4910 |
26851 |
163111 |
659853 |
|
||||
Mean |
4910 |
24344 |
147075 |
634285 |
|
||||
Evaluation after 72 hours
Statistically significant differences of growth rates and yield compared to
control values are marked (+), not significant differences are marked (-).
Nominal concentration |
Replicate |
Growth rate |
Rate-related inhibition |
Yield |
Inhibition of yield |
||
[mg/L] |
No. |
[d-1] |
[%] |
[cells/mL] |
[%] |
||
1.00 |
1 |
1.66 |
-2.40 |
706395 |
-12.2 |
||
2 |
1.69 |
-4.09 |
767088 |
-21.9 |
|||
3 |
1.64 |
-1.37 |
671495 |
-6.69 |
|||
4 |
1.68 |
-3.79 |
756019 |
-20.1 |
|||
5 |
1.65 |
-1.59 |
678807 |
-7.85 |
|||
6 |
1.65 |
-2.00 |
692584 |
-10.0 |
|||
Mean |
(+)* |
1.66 |
-2.54 |
(+)* |
712065 |
-13.1 |
|
Control |
1 |
1.63 |
646988 |
||||
2 |
1.61 |
603491 |
|||||
3 |
1.65 |
688962 |
|||||
4 |
1.58 |
561889 |
|||||
5 |
1.62 |
619976 |
|||||
6 |
1.63 |
654943 |
|||||
Mean |
1.62 |
629375 |
|||||
Section-by-Section and Average
Specific Growth Rates of the Control Group
(0 – 72 hours)
Replicate No. |
Specific growth rate [d-1] |
Mean (0 - 72 h) |
SD ± |
CV |
Mean CV [%] |
|||
section-by-section |
||||||||
0 - 24 hours |
24 - 48 hours |
48 - 72 hours |
||||||
Control |
1 |
1.63 |
1.74 |
1.52 |
1.63 |
0.112 |
6.88 |
11.0 |
2 |
1.58 |
1.84 |
1.40 |
1.61 |
0.220 |
13.7 |
||
3 |
1.52 |
1.88 |
1.55 |
1.65 |
0.195 |
11.8 |
||
4 |
1.52 |
1.83 |
1.40 |
1.58 |
0.222 |
14.0 |
||
5 |
1.65 |
1.71 |
1.50 |
1.62 |
0.109 |
6.74 |
||
6 |
1.70 |
1.80 |
1.40 |
1.63 |
0.211 |
12.9 |
||
Mean |
1.62 |
|
||||||
SD ± |
0.02 |
|
||||||
CV [%] |
1.45 |
|
||||||
NOEC, LOEC, EC - values of PV-Echtbraun RL (0-72 hours)
based on nominal concentration [mg/L]
Rate-related Inhibition |
|
NOEC |
1.00 |
LOEC |
> 1.00 |
ErC10 |
> 1.00 |
ErC20 |
> 1.00 |
ErC50 |
> 1.00 |
Inhibition of Yield |
|
NOEC |
1.00 |
LOEC |
> 1.00 |
EyC10 |
> 1.00 |
EyC20 |
> 1.00 |
EyC50 |
> 1.00 |
Cell Densities
|
Nominal concentration |
Replicate |
Cell density [cells/mL] |
||||||
[mg/L] |
No. |
0 hours |
24 hours |
48 hours |
72 hours |
|
|||
1.00 |
1 |
4910 |
29017 |
165338 |
711305 |
|
|||
2 |
4910 |
21642 |
135977 |
771998 |
|
||||
3 |
4910 |
22620 |
135753 |
676405 |
|
||||
4 |
4910 |
26607 |
173530 |
760929 |
|
||||
5 |
4910 |
27315 |
163950 |
683717 |
|
||||
6 |
4910 |
24681 |
134846 |
697494 |
|
||||
Mean |
4910 |
25314 |
151566 |
716975 |
|
||||
Control |
1 |
4910 |
24999 |
142808 |
651898 |
|
|||
2 |
4910 |
23784 |
149659 |
608401 |
|
||||
3 |
4910 |
22508 |
146782 |
693872 |
|
||||
4 |
4910 |
22454 |
139897 |
566799 |
|
||||
5 |
4910 |
25470 |
140194 |
624886 |
|
||||
6 |
4910 |
26851 |
163111 |
659853 |
|
||||
Mean |
4910 |
24344 |
147075 |
634285 |
|
||||
Evaluation after 72 hours
Statistically significant differences of growth rates and yield compared to
control values are marked (+), not significant differences are marked (-).
Nominal concentration |
Replicate |
Growth rate |
Rate-related inhibition |
Yield |
Inhibition of yield |
||
[mg/L] |
No. |
[d-1] |
[%] |
[cells/mL] |
[%] |
||
1.00 |
1 |
1.66 |
-2.40 |
706395 |
-12.2 |
||
2 |
1.69 |
-4.09 |
767088 |
-21.9 |
|||
3 |
1.64 |
-1.37 |
671495 |
-6.69 |
|||
4 |
1.68 |
-3.79 |
756019 |
-20.1 |
|||
5 |
1.65 |
-1.59 |
678807 |
-7.85 |
|||
6 |
1.65 |
-2.00 |
692584 |
-10.0 |
|||
Mean |
(+)* |
1.66 |
-2.54 |
(+)* |
712065 |
-13.1 |
|
Control |
1 |
1.63 |
646988 |
||||
2 |
1.61 |
603491 |
|||||
3 |
1.65 |
688962 |
|||||
4 |
1.58 |
561889 |
|||||
5 |
1.62 |
619976 |
|||||
6 |
1.63 |
654943 |
|||||
Mean |
1.62 |
629375 |
|||||
Section-by-Section and Average
Specific Growth Rates of the Control Group
(0 – 72 hours)
Replicate No. |
Specific growth rate [d-1] |
Mean (0 - 72 h) |
SD ± |
CV |
Mean CV [%] |
|||
section-by-section |
||||||||
0 - 24 hours |
24 - 48 hours |
48 - 72 hours |
||||||
Control |
1 |
1.63 |
1.74 |
1.52 |
1.63 |
0.112 |
6.88 |
11.0 |
2 |
1.58 |
1.84 |
1.40 |
1.61 |
0.220 |
13.7 |
||
3 |
1.52 |
1.88 |
1.55 |
1.65 |
0.195 |
11.8 |
||
4 |
1.52 |
1.83 |
1.40 |
1.58 |
0.222 |
14.0 |
||
5 |
1.65 |
1.71 |
1.50 |
1.62 |
0.109 |
6.74 |
||
6 |
1.70 |
1.80 |
1.40 |
1.63 |
0.211 |
12.9 |
||
Mean |
1.62 |
|
||||||
SD ± |
0.02 |
|
||||||
CV [%] |
1.45 |
|
||||||
- lncrease of biomass in the control during 72 h was 204 fold (criterion - not less than 16 fold)
- The coefficient of variation of the mean specific growth rate among the replicates in the control over the whole test period (test initiation - test termination) was 1.0% (criterion - not more than 7%)
- The mean of the replicate coefficients of variation in the section-by-section growth rate was 25.6 % (criterion - not exceeding 35%)
- lncrease of biomass in the control during 72 h was 204 fold (criterion - not less than 16 fold)
- The coefficient of variation of the mean specific growth rate among the replicates in the control over the whole test period (test initiation - test termination) was 1.0% (criterion - not more than 7%)
- The mean of the replicate coefficients of variation in the section-by-section growth rate was 25.6 % (criterion - not exceeding 35%)
Description of key information
Red disazo condensation pigments are with high probability not harmful to aquatic algae.
No toxic effects occur at the range of the substances solubility.
EC50 (72h) and EC10 (72h) >= 1 mg/L (nominal) for growth rate of Desmodesmus subspicatus (OECD 201)
Key value for chemical safety assessment
Additional information
The toxicity on aquatic algae was investigated for two ‘red disazo condensation pigments’(CAS 68516-75-6 and CAS 71566-54-6) [DR.U.NOACK-LABORATORIEN, 2012; lnstitute of lndustrial Organic Chemistry, Branch Pszczyna, 2012]. The studies were conducted with the green algae species Desmodesmus subspicatus (CAS 68516-75-6) and Pseudokirchneriella subcapitata (CAS 71566-54-6). Based on the low water solubility of the test item, saturated stock solutions were prepared and the tests were conducted as limit tests with nominal concentrations of 1 mg/L (CAS 68516-75-6) and 10 mg/L (CAS 71566-54-6), respectively. After 72 hours of exposure no toxic effects on algae observed, neither in the study with Desmodesmus subspicatus nor in the study with Pseudokirchneriella subcapitata.
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