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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
Comparable to internationally accepted guidelines.
Deviations:
no
Principles of method if other than guideline:
Method A-11
GLP compliance:
no
Specific details on test material used for the study:
FAT 40045/C
Purity: 100 %
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: Zebrafish
Details on test organisms:
10 animals were used per concentration.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
23 °C
pH:
7.8 - 7.9
Dissolved oxygen:
8.6 - 8.7
Nominal and measured concentrations:
300 and 1000 mg/L
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
After 96 hours of exposure, 10/10 and 5/10 fish survived at 300 and 1000 mg/L, respectively.
Validity criteria fulfilled:
no
Conclusions:
The 96h LC50 of FAT 40045/C in this short term fish toxicity test was determined to be 1000 mg/L.
Executive summary:

The short term fish toxicity test was performed using a methodology equivalent or similar to OECD Guideline 203. Groups of fish (containing 10 each) were exposed to FAT 40045/C for 96 hours at following concentrations: 300 and 1000 mg/L After 96 hours of exposure, 10/10 and 5/10 fish survived at 300 and 1000 mg/L, respectively. Based on the findings of the study, the LC50 of FAT 40045/C in this short term fish toxicity test was determined to be 1000 mg/L.

Description of key information

In the study designated as key, groups of zebra fish (containing 10 each) were exposed at test concentrations 300 or 1000 mg/L for a duration of 96 h. The number of surviving fish was recorded at 1, 4, 24, 48, 72 and 96 h. Mortality was observed only at the high concentration, with two fish dying within 48 h and 3 more within 96 h. Under the test conditions, the 96 and 48 h LC0 was 300 mg/L, whereas the 96 and 48 h LC50 and LC100 were both >1000 mg/L. The outcome of supporting studies available, also supports the conclusion that Reactive Blue 72 has low toxicity to fish when exposed for short term.

Key value for chemical safety assessment

LC50 for freshwater fish:
1 000 mg/L

Additional information