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Toxicity to microorganisms

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Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From April 22, 1993 to April 23, 1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Reference:
Composition 1
Qualifier:
according to
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Specific details on test material used for the study:
- Lot No.: E061733111
- Composition of test material, percentage of components: ca. 80% octadecyl trimethyl ammonium chloride, ca. 20% water and isopropanol
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Test temperature:
20-22°C
pH:
7.1
Nominal and measured concentrations:
Concentrations (nominal): 0.06 - 15.63 mg/L, corresponding to 0.05 - 12.50 mg/L based on active ingredient.
Reference substance (positive control):
yes
Remarks:
2,4-dichlorophenol
Key result
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
0.48 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Key result
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
0.88 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Duration:
16 h
Dose descriptor:
EC100
Effect conc.:
1.84 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Validity criteria fulfilled:
yes
Conclusions:
Under the study conditions, the 16h EC10, EC50 and EC100 for P. putida were 0.6, 1.1 and 2.3 mg/L (nominal). This was equivalent to 0.48, 0.88 and 1.84 mg/L, respectively, based on active ingredient.
Executive summary:

A study was conducted to determine the toxicity to microorganisms of the test substance according to DIN 38412-8, in compliance with GLP. The experiment was performed in Pseudomonas putida. Microorganisms were exposed to the test substance at nominal concentrations of 0.06 - 15.63 mg/L (corresponding to 0.05 - 12.50 mg a.i./L based on active ingredient) for 16 hours. A positive control, 2,4 dichlorophenol, was also included in the study. No analytical determination of the test substance was performed. Under the study conditions, the 16h EC10, EC50 and EC100 for P. putida were 0.6, 1.1 and 2.3 mg/L (nominal). This was equivalent to 0.48, 0.88 and 1.84 mg/L, respectively (Voelskow, 1993).

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
From March 06, 1997 to March 12, 1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 1
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Specific details on test material used for the study:
- Composition of test material, percentage of components: ca. 80% alkyl trimethyl ammonium chloride
- Lot No.: 1061962182
- Physical state: pellets
- Other: chemical formula: C21H46NCl
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- From community water treatment plant in Hildesheim, Germany
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
18-22°C
Nominal and measured concentrations:
Nominal: 0, 5.8, 10, 18, 32, 58 and 100 mg/L, corresponding to 0, 4.6, 8.0, 14.4, 25.6, 46.4 and 80 mg a.i./L (based on ca. 80% a.i. in test substance)
Reference substance (positive control):
yes
Remarks:
Copper sulfate pentahydrate
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
31.2 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
43.2 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 41.6 - 45.6 mg/L
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
60 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Remarks:
(based on ca. 80% ai in test substance)
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Validity criteria fulfilled:
yes
Conclusions:
Under the study conditions, the 3h EC20, EC50 and EC80 (in activated sludge) were 39 mg/L, 54 mg/L and 75 mg/L (nominal). This was equivalent to 31.2, 43.2 and 60.0 mg a.i./L (purity of 80%).
Executive summary:

A study was conducted to determine the toxicity tomicroorganisms of the test substance according to OECD Guideline 209, in compliance with GLP. In this respiration inhibition test, activated sludge was exposed to the test substance at nominal concentrations of 0, 5.8, 10, 18, 32, 58 and 100 mg/L, corresponding to 0, 4.6, 8.0, 14.4, 25.6, 46.4 and 80 mg a.i./L (based on ca. 80% active ingredient) for 3 hours. A positive control, copper sulfate pentahydrate was included in the study. No analytical determination of the test substance was performed. Under the study conditions, the 3h EC20, EC50 and EC80 (in activated sludge) were 39 mg/L, 54 mg/L and 75 mg/L (nominal). This was equivalent to 31.2, 43.2 and 60.0 mg a.i./L (Noack, 1997).

Description of key information

Key value for chemical safety assessment

EC50 or LC50 for microorganisms:
0.88 mg/L
EC10, LC10 or NOEC for microorganisms:
0.48 mg/L

Additional information

Study 1. A study was conducted to determine the toxicity to microorganisms of the test substance according toDIN 38412-8, in compliance with GLP.The experiment was performed in Pseudomonas putida. Microorganisms were exposed to the test substance at nominal concentrations of 0.06 - 15.63 mg/L (corresponding to 0.05 - 12.50 mg a.i./L based on active ingredient) for 16 hours. A positive control, 2,4 dichlorophenol, was also included in the study. No analytical determination of the test substance was performed.Under the study conditions, the 16h EC10, EC50 and EC100 for P. putida were 0.6, 1.1 and 2.3 mg/L (nominal). This was equivalent to 0.48, 0.88 and 1.84 mg/L, respectively (Voelskow, 1993).

Study 2. A study was conducted to determine the toxicity tomicroorganisms of the test substance according to OECD Guideline 209, in compliance with GLP. In this respiration inhibition test, activated sludge was exposed to the test substance at nominal concentrations of 0, 5.8, 10, 18, 32, 58 and 100 mg/L, corresponding to 0, 4.6, 8.0, 14.4, 25.6, 46.4 and 80 mg a.i./L (based on ca. 80% active ingredient) for 3 hours. A positive control, copper sulfate pentahydrate was included in the study. No analytical determination of the test substance was performed.Under the study conditions, the 3h EC20, EC50 and EC80 (in activated sludge) were 39 mg/L, 54 mg/L and 75 mg/L (nominal). This was equivalent to 31.2, 43.2 and 60.0 mg a.i./L (Noack, 1997).