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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
From 1980 to 1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Remarks:
RA study
Justification for type of information:
Refer to the section 13 for details on the read across justification. The long-term toxicity to aquatic invertebrates study with the read across substance is considered sufficient to fulfil the information requirements as further explained in the provided endpoint summary.
Reference:
Composition 1
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
no
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material (as cited in study report): Mono tallow trimethyl ammonium chloride (MTTMAC) (also known as C16-18 and C18-unsatd. TMAC).
- Analytical purity: 48.4%
Analytical monitoring:
yes
Vehicle:
yes
Details on test solutions:
Preparation and application of test solution (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Isopropanol
Test organisms (species):
Daphnia magna
Details on test organisms:
Test organisms
- Common name: Daphnia magna (< 24 h old)

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Hardness:
Water hardness value: 332 mg CaCO3/L
Upper water hardness value: 364 mg CaCO3/L
Test temperature:
Temperature value: 19.2°C
Upper temperature value: 21.2°C
pH:
pH Value: 7.5
Upper pH Value: 8.3
Dissolved oxygen:
8.8 - 9.2 mg/L
Nominal and measured concentrations:
- Nominal concentrations (μg/L):
Southwest well water: 2.5, 5.0, 10.0, 20.0, 40.0 and 80.0 μg/L
River water: 74.4, 110.4, 146.4, 218.4, 290.4 and 578.4 μg/L
- Measured concentrations (μg/L; values represent the geometric mean of the 0- and 24-h concentration analyses):
Southwest well water: 1.6, 3.1, 6.8, 14.6, 30.6 and 60.8 μg/L
River water: 35.7, 53.4, 68.3, 99.1, 122.3 and 309.3 μg/L
Details on test conditions:
Testem
- Renewal rate of test solution (frequency/flow rate): Static (daily renewal)

Effect parameters measured (with observation intervals if applicable) : Mortality was monitored daily and the number of young produced in each beaker was recorded after which they were discarded. Temperature was recorded daily and pH, dissolved oxygen and hardness were determined on alternate days in control waters, both fresh and 24h old. Daphnid 21-day length was also determined by the use of an ocular micrometer measuring from the base of the spine to the apex of the helmet.

Vehicle control: yes (isopropanol)
Reference substance (positive control):
no
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.007 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Basis for effect:
other: mortality and total number of young produced
Remarks on result:
other: test conducted with Southwest well water.
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.099 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Basis for effect:
other: mortality and total number of young produced
Remarks on result:
other: test conducted with river water.
Results with reference substance (positive control):
-
Reported statistics and error estimates:
Mortality data were analysed by probit analysis to derive a 21-d NOEC value and associated 95% confidence interval. t-tests were used to analyse statistically significant differences in other tested parameters including days to first reproduction, total young production, mean brood size, and 21-d length.
Conclusions:
Based on the results, the 21d NOECs were 0.0068 and 0.099 mg a.i./L in Southwest well and river water, respectively. The number of days until the first reproduction were similar across between the Southwest well (11 d) and the river water (8-9 d) groups.
Executive summary:

A study was conducted to determine the long-term toxicity to aquatic invertebrates of the read across substance, quaternary ammonium compounds, trimethyltallow alkyl, chlorides (C16-18 and C18-unsatd. TMAC), according to OECD Guideline 211. Daphnids were exposed to six concentrations (dissolved in isopropanol) in a 21-day static-daily renewal test in three different water types (laboratory blended water, Southwest well water and river water). Analytical dose verification was performed. Measured concentrations (geometric mean of the 0 and 24 h concentrations) were found to be equivalent to 1.6, 3.1, 6.8, 14.6, 30.6 and 60.8 μg a.i./L in Southwest well water, and 35.7, 53.4, 68.3, 99.1, 122.3 and 309.3 μg a.i./L in river water. The test in blended water was discontinued after 14 d due to inadequate reproduction by control organisms. Mortality was monitored daily and the number of young produced in each beaker was recorded. Based on the results, the 21 d NOECs were 0.0068 and 0.099 mg a.i./L in Southwest well and river water, respectively. The number of days until the first reproduction were similar across between the Southwest well (11 d) and the river water (8-9 d) groups (Valentine, 1992).

Description of key information

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
0.007 mg/L

Additional information

A study was conducted to determine the long-term toxicity to aquatic invertebrates of the read across substance, quaternary ammonium compounds, trimethyltallow alkyl, chlorides (C16-18 and C18-unsatd. TMAC), according to OECD Guideline 211. Daphnids were exposed to six concentrations (dissolved in isopropanol) in a 21-day static-daily renewal test in three different water types (laboratory blended water, Southwest well water and river water). Analytical dose verification was performed. Measured concentrations (geometric mean of the 0 and 24 h concentrations) were found to be equivalent to 1.6, 3.1, 6.8, 14.6, 30.6 and 60.8 μg a.i./L in Southwest well water, and 35.7, 53.4, 68.3, 99.1, 122.3 and 309.3 μg a.i./L in river water. The test in blended water was discontinued after 14 d due to inadequate reproduction by control organisms. Mortality was monitored daily and the number of young produced in each beaker was recorded. Based on the results, the 21d NOECs were 0.0068 and 0.099 mg a.i./L in Southwest well and river water, respectively. The number of days until the first reproduction were similar across between the Southwest well (11 d) and the river water (8-9 d) groups (Valentine, 1992).