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EC number: 203-929-1
CAS number: 112-03-8
study was conducted to determine the biodegradation of the read across
substance, quaternary ammonium compounds, benzyl C12-C16 (even
numbered)-alkyldimethyl chlorides (C12-16 ADBAC), in a loamy soil. The
study comprised two treatments - test substance and chemical blank
control, each with three replicates - and was carried out in biometers
containing approximately 50 g dry weight equivalents of soil. The test
substance was added in appropriate amounts of deionised water not
exceeding that required for bringing the soils to 50 to 70% of the
maximum moisture capacity. The soils were maintained at this moisture
level by the addition of deionised water, when necessary. Loam was added
to the biometers after the test solutions to facilitate uniform
moistening of the soils by capillary action. Test substance was added at
a concentration of 10 mg carbon per 50 g soil. The side tube of the
biometer contained 20 mL 0.2 M KOH for absorbing carbon dioxide produced
by the microorganisms. The test was incubated at 22 ± 3°C and run for
approximately 90 d. The theoretical CO2 production of the test substance
was calculated from its carbon content. The amounts of carbon dioxide
produced was calculated by subtracting the mean carbon dioxide
production in the test systems containing the test substance and the
mean carbon dioxide production level in the control blank.
Biodegradation was calculated as the ratio of experimental carbon
dioxide production to theoretical carbon dioxide production [ThCO2P].
Based on the results of the read across study, the test substance was
64% biodegraded in loam at Day 70. This percentage of the theoretical
carbon dioxide production presumes complete mineralisation. The DT50 was
estimated to be 40 days (van Ginkel, 1994).
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