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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD guideline study incl. GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
The test substance is poorly water soluble, therefore a saturated stock solution was prepared using a liquid-liquid saturator technique.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Reference substance (positive control):
yes
Remarks:
sodium chloride (NaCI)

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOELR
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EL100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Results with reference substance (positive control):
The EC50(24h) of the reference substance sodium chloride was 5.25 mg/L (experiment date: 09 Jan 2013, project number: 50E0063/04E004).
This result is within the range of 3.88 – 7.22 mg/L [Ref. 9, 10] and indicates that the culture of Daphnia magna used in this study is responding normally to toxic stress.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance is not harmful to aquatic invertebrates.