Reaction products of phosphoryl trichloride and 2-methyloxirane

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

Static bioassay: method not specified

GLP compliance:

no

Specific details on test material used for the study:

- Common name: TCPP
- Product name: Antiblaze 80 (AB-80), Purity >95%

Analytical monitoring:

yes

Details on sampling:

The samples were acidified with 6 N HCl to pH 2, refrigerated and afterwrads extracted with metylene chloride. Samples were taken at test start (0 h) and at test end (48 h).

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna: The primary culture originated from Analytical Bio-Chemistry Laboratories (ABC). Only first-instar Daphnia less than 24 hours
old were used in the study.
During culturing, the daphnids were fed a nutrient anriched solution containing green algae. The Daphnia were not fed during of the study.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

CaCO3 262 mg/L

Test temperature:

22°C

pH:

8.36 - 8.59

Dissolved oxygen:

8.3 - 8.8 mg/L

Nominal and measured concentrations:

Nominal concentration: 33.5, 67, 335, 502 and 670 mg/L
measured 0 h: 43.4, 23.6, 173, 193 and 266 mg/L
measured 48 h: 25.9, 65.0, 176, 233 and 281 mg/L

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

131 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 65-176

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

209 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 67-335

Details on results:

Analytical monitoring was performed, and the result relate to measured concentrations as the recovery rate was in the range between 42% and 97%.

Concentration of test substance in water collected at t = 0 hrs. and t = 48 hrs. in all test chambers in relation to the nominal concentration

Container	Nominal conc. in mg/L	Meassured conc. t = 0 h in mg/L	Recovery rate in %	Meassured conc. t = 48 h in mg/L	Recovery rate in %
1	Control
2	33.5	43.4	130
2	33.5			25.9	77
3	67	23.6	35
3	67			65.0	97
4	335	173	52
4	335			176	53
5	502	193	38
5	502			233	46
6	670	266	40
6	670			281	42

Analytical monitoring was performed, and the result relate to measured concentrations as the recovery rate was in the range between 42% and 97%. The 48 hour computer estimated EC50 were calculated using the binomial probability test and nominal dose levels was 209 mg/L. The 48 hour computer estimated EC50 were calculated using the binomial probability test and actual test concentration was 131 mg/L.

Validity criteria fulfilled:

yes

Remarks:

No abnormal behaviour of test population in control

Conclusions:

Short-term toxicity test of TCPP to invertebrates (Dapnia magna) was assessed in a static bioassay with analytical monitoring. After 48 hours of exposure an EC50 of 131 mg/L (measured) is obtained.

Executive summary:

The study was not performed according to guideline but meets generally accepted scientific principles.

An analytical monitoring was performed, and the result relate to measured concentrations as the recovery rate was in the range between 42% and 97%. The 48 hour computer estimated EC50 were calculated using the binomial probability test and nominal dose levels was 209 mg/L. The 48 hour computer estimated EC50 were calculated using the binomial probability test and actual test concentration was 131 mg/L.

Description of key information

OECD 201 (equiv.) (Meeks, 1985): 48h EC50 = 131 mg/L (meas.)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

131 mg/L

Additional information

The study was not performed according to guideline but meets generally accepted scientific principles.

An analytical monitoring was performed, and the result relate to measured concentrations as the recovery rate was in the range between 42% and 97%. The 48 hour computer estimated EC50 were calculated using the binomial probability test and nominal dose level was 209 mg/L. The 48 hour computer estimated EC50 were calculated using the binomial probability test and actual test concentration was 131 mg/L.