L 130

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 - 16 July 2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted according with OECD Guideline No. 202 (draft 2000) and in accordance with GLP principles. The study is considered to be reliable without restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

(draft 2000)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Leuchtstoff L 130 (L 130)
- Physical state: Solid (powder)
- Stability under test conditions: Not available
- Storage condition of test material: room temperature, dark and dry
- Expiration date of the lot/batch: not applicable (according to the sponsor unlimited storable, when protected from humidity)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 (control) and 100 mg/L (loading rate)
- Sampling method: Not reported
- Sample storage conditions before analysis: The samples were stored at a temperature of ≤-18 °C until the beginning of analysis.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A total of 0.100 g L 130 were added to 1 L of dilution water. This stock solution was shaken for 48 hours and then filtered (0.45 µm). The resulting filtrate was the final solution used to expose daphnids.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Not reported

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water fleas
- Source: Landesanstalt für Umweltschutz Baden-Württemberg Griesbachstr. 1, D-76185 Karlsruhe
- Age at study initiation: <24 h
- Method of breeding: under standardised laboratory conditions
- Feeding during test: None

ACCLIMATION
- Acclimation period: Not specified
- Acclimation conditions (same as test or not): Yes
- Food type: Alga Desmodesmus subspicatus origin: Albrecht-v.-HalLer-Institut für Pflanzenwissenschaften, Niklausberger Weg 18, D-37073 Gottingen
- Amount: Daphnia of different age were fed with a defined suspension of algae:
1 day old: 0.5 ml/50 ml M4; 3 to 10 days old: 1.0 ml/50 ml M4; > 10 days old: 1.5 ml/50 ml M4
- Feeding frequency: daily
- Health during acclimation (any mortality observed): Not reported

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48

Test conditions

Hardness:

200 mg CaCO3/L

Test temperature:

19.9 - 20.3 °C

pH:

7.80 - 7.84

Dissolved oxygen:

8.47 - 9.08 mg/L

Nominal and measured concentrations:

Nominal: 0 (blank control) and 100 mg/L (loading rate)
Meaured (Sr content)
at 0h: <0.012 (blank control) and 0.452 mg Sr/L
at 48 h: <0.012 (blank control) and 0.442 mg Sr/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker with a capacity of 150 ml and filled with 100 ml of test solution
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Loading: 1 daphnid per 20 ml of test solution

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water according to ISO 6341
- Conductivity: 0.656 µS/cm
- Culture medium different from test medium: yes
- Intervals of water quality measurement: At 0 and 48 h the pH, dissolved oxygen and test temperature were measured.

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): At 24 and 48 h the number of immobilised Daphnia per vessel (including discoloration, anomalies) were determined.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Basis for effect:

mobility

Remarks on result:

other: EC50 > maximum soluble concentration in test medium

Details on results:

Measured concentrations:
- At t=0 h, 0.452 mg Sr/L was measured
- At t=48 h, 0.442 mg Sr/L was measured
- At 48 h the test concentration remained within ± 20% of the initial measured concentration.

- Biological results: No mortality or any visible signs of abnormality were observed during the entire test period.

- Validity criteria
Immobilised daphnids: ≤10% in the control
Oxygen concentration at the end of the test: ≥3 mg/L in control and test vessels

Results with reference substance (positive control):

- The 48 h EC50 for potassium dichromate was 1.25 mg/L.

Reported statistics and error estimates:

- No statistical evaluation was performed.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No effects were observed and thus the 48h-EC50 was considered to exceed the maximum solubility of the test substance in medium.

Executive summary:

The acute toxicity to Daphnia magna was examined under static conditions in a test that was carried out according to OECD 202 (draft of 2000) and GLP. The test was carried out as a limit test. An amount of 100 mg of L 130 was added to 1 L of medium (dilution water according to ISO 6341). This solution was shaken for 48 hours and then filtered (0.45 µm). The resulting filtrate was the final solution used to expose daphnids. At the beginning and at the end of the exposure period, the concentration strontium (Sr) was measured. The measured concentration was 0.452 mg Sr/L at the start and 0.442 mg Sr/L at the end of the test. No effects were oberved and thus the 48h-EC50 was considered to exceed the maximum solubility of the test substance in medium. The validity criteria were fulfilled.