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EC number: 692-448-4 | CAS number: 170621-28-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 Nov - 06 Dec 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted 22 Jul 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- adopted 30 May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Rheinland-Pfalz, Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test item was directly added to the test vessels. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant D-67435 NW-Lachen-Speyerdorf.
- Pretreatment: Upon arrival in the test facility, the sludge was filtrated, washed with tap water and re-suspended in tap water. The activated sludge was aerated until usage in the test and fed daily with 50 ml/L synthetic sewage feed.
- Initial biomass concentration (first experiment): 2.90 g suspended solids/L (1.45 g suspended solids/L in the test)
- Initial biomass concentration (second experiment): 3.00 g suspended solids/L (1.50 g suspended solids/L in the test) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 1.08 mmol/L (Analysis of drinking water, Sep 2012)
- Test temperature:
- 18.9 – 20.0 °C (first experiment)
18.0 – 18.8 °C (second experiment) - Nominal and measured concentrations:
- control, 1, 10, 100 and 1000 mg/L (nominal) (first experiment)
control, 320 and 1000 mg/L (nominal) (second experiment) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Glass beakers
- Aeration: purified air, using Pasteur pipettes, flow approx. 0.75 L/min
- No. of vessels per concentration (replicates, first experiment): 5 for 1000 mg/L; for other concentrations 1 replicate
- No. of vessels per concentration (replicates, second experiment): 5 for both 1000 and 320 mg/L
- No. of vessels per control (replicates, both experiments): 6
- Biomass loading rate first experimenmt): 1.45 g suspended solids/L
- Biomass loading rate first experimenmt): 1.50 g suspended solids/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Total organic carbon: 0.4 mg/L
- Metals: Al 0.006 mg/L; Cu 0.002 mg/L; others < LOQ
- Pesticides: < LOD
- Chloride: 14.0 mg/L
- Conductivity: 330 µS/cm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): O2 consumption, measured at the end of exposure - Reference substance (positive control):
- yes
- Remarks:
- 3.5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 16 Nov 2012: 3h EC50 = 6.6 mg/L; 06 Dec 2012: 3h EC50 = 7.8 mg/L - Reported statistics and error estimates:
- For the treatments with the test item concentrations 1000 and 320 mg/L (experiment 2 only), it was tested whether the differences between treatment and control were significant. For this determination, the values of the O2 consumption were used. In order to select a suitable test for significance, it was checked whether equality of variance was given. If equality of variance is given, the t-test is used; else, the WEIR test is used. The difference between treatment 1000 mg/L and the control can be considered as significant as the calculated t-value lay above the significance level and O2 consumption in the treatment was higher than in the control. Therefore, the NOEC is stated as 320 mg/L. The highest treatment of nominal 1000 mg/L showed inhibition values below 10 %, therefore EC10 and EC50 lies above 1000 mg/L.
Reference
Table 1. O2 -consumption, inhibition; first experiment
Vessel No. |
Content |
Concentration in mg/L |
O2 consumption in mg/(L*min.) |
O2 consumption in mg/(L*h) |
Inhibition in % |
pH |
1 |
Control |
0 |
0.7928 |
47.566 |
0 |
7.7 |
2 |
Control |
0 |
0.7550 |
45.300 |
0 |
7.7 |
3 |
Positive Control |
5 |
0.4845 |
29.069 |
38.7 |
7.8 |
4 |
Positive Control |
10 |
0.2772 |
16.631 |
64.9 |
7.8 |
5 |
Positive Control |
20 |
0.1280 |
7.679 |
83.8 |
7.8 |
6 |
Positive Control |
40 |
0.0638 |
3.826 |
91.9 |
7.8 |
7 |
Control |
0 |
0.8041 |
48.248 |
0 |
7.6 |
8 |
Control |
0 |
0.7685 |
46.112 |
0 |
7.7 |
9-16 |
* |
* |
* |
* |
* |
* |
17 |
Test Item |
1003.6 |
0.7188 |
43.130 |
9.0 |
8.2 |
18 |
Test Item |
1001.8 |
0.7437 |
44.624 |
5.8 |
8.2 |
19 |
Test Item |
1000.0 |
0.7522 |
45.132 |
4.8 |
8.1 |
20 |
Test Item |
999.8 |
0.7395 |
44.368 |
6.4 |
8.2 |
21 |
Test Item |
1002.2 |
0.7388 |
44.330 |
6.5 |
8.2 |
22 |
Test Item |
100 |
0.7886 |
47.313 |
0.2 |
7.7 |
23 |
Test Item |
11 |
0.8049 |
48.296 |
-1.9 |
7.6 |
24 |
Test Item |
1 |
0.7849 |
47.094 |
0.6 |
7.6 |
25 |
Control |
0 |
0.8184 |
49.106 |
0 |
7.5 |
26 |
Control |
0 |
0.8001 |
48.007 |
0 |
7.6 |
*other test items were measured in vessels 9 – 16
Table 4. O2 -consumption, inhibition; second experiment
Vessel No. |
Content |
Concentration in mg/L |
O2 consumption in mg/(L*min.) |
O2 consumption in mg/(L*h) |
Inhibition in % |
pH |
1 |
Control |
0 |
0.5673 |
34.039 |
0 |
7.8 |
2 |
Control |
0 |
0.5513 |
33.076 |
0 |
7.8 |
3 |
Positive Control |
5 |
0.3820 |
22.921 |
33.6 |
7.9 |
4 |
Positive Control |
10 |
0.2244 |
13.466 |
61.0 |
7.9 |
5 |
Positive Control |
20 |
0.1159 |
6.956 |
79.9 |
8.0 |
6 |
Positive Control |
40 |
0.0674 |
4.042 |
88.3 |
8.0 |
7 |
Control |
0 |
0.5858 |
35.148 |
0 |
7.8 |
8 |
Control |
0 |
0.5727 |
34.364 |
0 |
7.8 |
9 |
Test Item |
1000.2 |
0.5311 |
31.864 |
7.7 |
8.4 |
10 |
Test Item |
1001.2 |
0.5270 |
31.621 |
8.4 |
8.4 |
11 |
Test Item |
1000.0 |
0.5384 |
32.303 |
6.5 |
8.4 |
12 |
Test Item |
1001.0 |
0.5343 |
32.061 |
7.2 |
8.4 |
13 |
Test Item |
1001.6 |
0.5336 |
32.019 |
7.3 |
8.4 |
14 |
Test Item |
320.8 |
0.5488 |
32.925 |
4.7 |
8.0 |
15 |
Test Item |
320.0 |
0.5540 |
33.238 |
3.8 |
8.0 |
16 |
Test Item |
321.0 |
0.5630 |
33.778 |
2.2 |
7.9 |
17 |
Test Item |
321.6 |
0.5673 |
34.038 |
1.4 |
8.0 |
18 |
Test Item |
320.6 |
0.5710 |
34.257 |
0.8 |
8.0 |
19 |
Control |
0 |
0.5808 |
34.846 |
0 |
7.8 |
20 |
Control |
0 |
0.5957 |
35.741 |
0 |
7.8 |
Description of key information
EC50 (3h) > 1000 mg/L (nominal) (OECD 209, activated sludge)
Key value for chemical safety assessment
Additional information
The determination of the inhibition of the respiration of activated sludge exposed to aluminium magnesium vanadium oxide was conducted according to OECD 209 and GLP (2013). Two valid experiments were performed. In the first experiment, the test item was exposed to four concentrations (1, 10, 100 and 1000 mg/L). Duration of the test was three hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. For the determination of the NOEC, a second experiment was performed. Because of low inhibition at the highest treatment and low solubility in the test medium, no full test was conducted. The second experiment was performed in the same way like the first, but using two concentrations, namely 1000 and 320 mg/L (nominal). The 3h NOEC is 320 mg/L and EC10 and EC50 are > 1000 mg/L.
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