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EC number: 700-700-2 | CAS number: 1369492-55-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 February 2011 to 02 March 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant, guideline study, no restrictions, fully adequate for assessment.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: No stock solution was made due to the low water solubility of the test item. 500.0mg of test substance was weighed by means of an analytical balance and transferred to the designated test vessels with 284 mL tap water. The test item was mixed into the tap water by ultrasonic treatment for fifteen minutes and intense stirring for three hours at room temperature in the dark to dissolve a maximum amount of the test item and/or disperse it as homogeneously as possible.
- Eluate: Not applicable
- Controls: Two blank controls of tap water containing synthetic sewage feed and inoculum. Reference item 3, 5-dichlorophenol was tested in parallel at three concentrations under identical conditions as a toxicity control.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Appearance of test media showed that the single test concentration was clearly above the water solubility limit of the test item. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: No
- Method of cultivation: Aerobic activated sludge from a wastewater treatment plant (ARA Ergolz II, Füllinsdorf / Switzerland) treating predominantly domestic wastewater was collected. The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated:
- Preparation of inoculum for exposure: Based on the wet to dry weight ratio, an aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 3 g dry material per litre. During the holding period of three days prior to use, the sludge was fed daily with 50 mL synthetic sewage feed* per liter and was kept at room temperature under continuous aeration until use. Before use, the dry weight of the activated sludge was measured again in the inoculum used for the test. The pH of the activated sludge inoculum was 7.2.
* Synthetic sewage feed:
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl filled up to a final volume of 1 liter
0.4 g CaCl2 × 2H2O with deionized water
0.2 g MgSO4 × 7H2O
2.8 g K2HPO4
Made up to 1 litre with deionized water.
- Pretreatment: None
- Initial biomass concentration: 2.2 g/L dry weight (corresponding to about 0.9 g dry material per litre test medium). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- None reported
- Hardness:
- Not reported
- Test temperature:
- 19ºC to 20ºC
- pH:
- 7.3 to 8.6
- Dissolved oxygen:
- 7.6 mg/L to 8.6 mg/L after 3-hour incubation period and just before respiration rate measurements
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Test substance nominal concentrations: 1000 mg/L.
Reference substance nominal concentrations: 5, 16 and 50 mg 3, 5-dichlorophenol/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Open
- Material, size, headspace, fill volume: 2000 mL glass beakers filled with 500 mL solution.
- Aeration: Continuous aeration by intense stirring with magnetic stirrer.
- Sludge concentration: 2.2 g/L dry weight (corresponding to about 0.9 g dry material per litre of test medium).
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- No. of vessels per reference item(replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Total organic carbon: Not reported
- Particulate matter: Not reported
- Metals: Not reported
- Pesticides: Not reported
- Chlorine: Not reported
- Alkalinity: Not reported
- Ca/mg ratio: Not reported
- Conductivity: Not reported
- Culture medium different from test medium: No.
- Intervals of water quality measurement: Not reported
OTHER TEST CONDITIONS
- Adjustment of pH: None
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Oxygen respiration rate measured continuously after 3 hour incubation period (see below for more details). - Reference substance (positive control):
- yes
- Remarks:
- 3, 5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Limit test.
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: At the test item concentration of 1000 mg/L, the test item was not completely dissolved in the test medium. Thus, the test concentration was clearly above the water solubility limit of the test item under the test conditions.
3-hour EC20, EC50 and EC80 could not be determined but where clearly higher than 1000 mg CA4920/L.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: The 3-hour EC50 of the reference item 3,5-dichlorophenol (positive control) was calculated to be 9 mg/L (the 95% confidence limits could not be calculated due to mathematical reasons). The 3-hour EC50 is within the guideline-recommended range of 5–30 mg/L, confirming suitability of the activated sludge used. - Reported statistics and error estimates:
- The 3-hour EC50 value of the reference item 3, 5-dichlorophenol was calculated by Probit Analysis
- Validity criteria fulfilled:
- yes
- Remarks:
- The oxygen consumption rates of the two controls is within 15% of each other and the 3-hour EC50 of the reference substance is within the guideline-recommended range of 5 - 30 mg 3, 5-dichlorophenol/L.
- Conclusions:
- In a limit test, the 3-hour NOEC value for the inhibitory effect of the test material on the respiration rate of aerobic activated sludge microorganisms was 1000 mg/L, based on nominal concentrations. The test concentrations was clearly above the water solubility of the test material under the test conditions. The test item has no inhibitory effect ( ≤ 10%) on the respiration rate of activated sludge after the incubation period of three hours at the test item concentration of 1000 mg/L.
- Executive summary:
The sensitivity of activated sludge microorganisms to the test material was determined in a GLP-compliant test performed to standard guidelines. In a limit test, the test item has no inhibitory effect on the respiration rate of activated sludge after the incubation period of three hours at the test item concentration of 1000 mg/L.. The 3-hour NOEC value was determined to be 1000 mg/L. The results are based on nominal concentrations and the test concentration was clearly above the water solubility of the test material under the test conditions.
Reference
Table 1. Influence of Test Item and 3, 5-Dichlorophenol (reference item) on the Oxygen Consumption of Activated Sludge | |||||||
Vessel no. | Nominal concentration of test chemical (mg/L) | Oxygen consumption rate (mg O2L-1min-1) | Inhibition (%) | pH values | Oxygen Concentration (mg O2/L | ||
start* | end* | start* | end* | ||||
Control | |||||||
A | 0 | 1.033 | 7.3 | 8.5 | 8.1 | 7.9 | |
B | 0 | 1.078 | 7.5 | 8.4 | 8.0 | 8.0 | |
Mean | 1.056 | ||||||
Deviation (%) | 4.3 | ||||||
Test Item | |||||||
5 | 1000 | 1.056 | -0.1 | 7.6 | 8.4 | 8.3 | 8.2 |
Reference item | |||||||
1 | 5 | 0.821 | 22.2 | 7.6 | 8.6 | 8.1 | 7.6 |
2 | 16 | 0.24 | 77.3 | 7.5 | 8.6 | 8.2 | 8.6 |
3 | 50 | 0.089 | 91.6 | 7.5 | 8.6 | 8.1 | 8.6 |
* start and end of the 3-hour incubation period | |||||||
-% inhibition: increased oxygen consumption rate relative to control |
Description of key information
In a limit test, the 3-hour NOEC value for the inhibitory effect of the test material on the respiration rate of aerobic activated sludge microorganisms was 1000 mg/L, based on nominal concentrations. The test concentrations was clearly above the water solubility of the test material under the test conditions. The test item has no inhibitory effect ( ≤ 10%) on the respiration rate of activated sludge after the incubation period of three hours at the test item concentration of 1000 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The sensitivity of activated sludge microorganisms to the test material was determined in a GLP-compliant test performed to standard guidelines. In a limit test, the test item has no inhibitory effect on the respiration rate of activated sludge after the incubation period of three hours at the test item concentration of 1000 mg/L.. The 3-hour NOEC value was determined to be 1000 mg/L. The results are based on nominal concentrations and the test concentration was clearly above the water solubility of the test material under the test conditions.
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