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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The degree of biodegradation of the test substance after an exposure period of 28 days was determined to be 20-30 % CO2/ThCO2. Therefore, the test substance is regarded to be inherently biodegradable. 

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable

Additional information

The study assessing the biodegradability of the test substance was performed in accordance with EU method C.4 -C, OECD guideline 301B, US EPA OPPTS 835.3110 guideline and International standard ISO 9439. Activated sludge from a municipal sewage plant was used as inoculum.

The degree of biodegradation after an exposure period of 28 days was 20-30 % CO2/ThCO2. The mean value of degree of biodegradation was calculated by the single values of two test assays at the end of exposure. The required pass level of degree of biodegradation of 60 % CO2/ThCO2 within 10 days was not fulfilled. Evaluation was conducted in accordance to criteria of the OECD Test Guideline 301 B. Based on the rate of biodegradation of 20-30 % CO2/ThCO2 at the end of exposure the test substance can be evaluated as partly or moderately biodegradable and inherently biodegradable. The results in this study are consistent with all validity criteria and the test is valid according to the guideline of this study. No deviations from the test guidelines or other incidents occurred during the course of the reported test which may have influenced the results.