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Mixture of hexa(sodium,lithium) 4-{[1,7-disulfonato-5-hydroxy-6-({4-[(6-methoxy-5-sulfonatobenzoheteromonocycle-2-yl)diazenyl]-5-alkyl-2-(3-sulfonatopropoxy)phenyl}diazenyl)naphthalen-2-yl]diazenyl}-5-hydroxy-1-(4-sulfonatophenyl)heteromonocycle-3-carboxylate and hexa(sodium,lithium) 4-{[1,7-disulfonato-5-hydroxy-6-({4-[(6-methoxy-7-sulfonatobenzoheteromonocycle-2-yl)diazenyl]-5-alkyl-2-(3-sulfonatopropoxy)phenyl}diazenyl)naphthalen-2-yl]diazenyl}-5-hydroxy-1-(4-sulfonatophenyl)heteromonocycle-3-carboxylate
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26-29 October 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted following the GLP principles, and according to the OECD and Japanese guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: "Alga growth inhibition test, Daphnia sp. acute immobilisation test, and fish acute test" Yakushokuhatsu No. 1121002, Heisei 15.11.13 Seikyoku No. 2, Kanpokihatsu No. 031121002, November 21, 2003; the latest revision November 20, 2006
- GLP compliance:
- yes
Test material
- Details on test material:
- - Name of test material (as cited in study report): E-BK105
- Substance type: black powder
- Physical state: solid
- Lot/batch No.: MB-1
- Storage condition of test material: room temperature, shaded, air-tight
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0; 1.0; 3.2; 10; 32 and 100
- Sampling method: from the middle layer of all vessels and mix
- Sample storage conditions before analysis: not reported
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The stock solution was prepapred by addition of 100 mg test substance to 1000 ml dilution water, resulting in a concentration of 100 mg/L. From this stock solution, 75 ml were taken and added in a 300 ml Erlenmeyer flask with air-permeable silicon cap. this was considered as the highest etst concentration of 100 mg/L. Next, by diluting the stock solution, series dilutions in 75 ml medium separared by a factor of 3.16, were prepared.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: ATCC22662
- Source (laboratory, culture collection): American Type Culture Collection
ACCLIMATION
- Acclimation period: 20-26 October 2010
- Culturing media and conditions (same as test or not): yes
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- other: static and shaking at 100 rpm
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 50 mg NAHCO3/L
- Test temperature:
- 22.0 - 22.4
- pH:
- 7.8 - 8.5
- Nominal and measured concentrations:
- Nominal: 0; 1.0; 3.2; 10; 32 and 100 mg/L
Measured: 0.95; 3.14; 9.86; 31.4; 99.2 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): open; air permeable cap
- Material, size, headspace, fill volume: glass, 300 ml
- Initial cells density: 5 x 10^3 cells/ml algae of exponential growth phase
- Control end cells density: 161 X 10^4 cells/ml (average)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, Gorham
OTHER TEST CONDITIONS
- Sterile test conditions: yes, every 6 months
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 110 to 120 uE/m2/s, fluorescent white
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 50% growth inhibition concentration and no observed effect concentration (0-72 h)
- Determination of cell concentrations: electronic particle counter
- Chlorophyll measurement: no
TEST CONCENTRATIONS
- Spacing factor for test concentrations: for the final test only 0; 1.0; 3.2; 10; 32 and 100 mg/L. The test solutions were colorless in control, dark blue in 1.0 and 3.2 mg/L and black in 10; 32 and 100 mg/L.
- Range finding study: yes
- Test concentrations:control; 0.10; 1.0; 10 mg/L
- Results used to determine the conditions for the definitive study: 46 % inhibition was observed at 10 mg/L in the range finding test. As the test substance was of black color, additional studies were conducted on effects of light absorption or interception caused by the substance on algal growth. the methods used were as follows:
1) Growth of algae under the light filtered through petri dishes filled with the 10 mg/L test solution (concentration group) was measured to investigate the effects the effect of light absorption or interception by test solutions and test cultures. Light filtered through petri dish filled with test medium was used as control.
2) The effect of light absorption or interception by test solution and test cultures was investigated by reducing the test solution volume, thus reducing the light path. One replicate 300 ml Erlenmeyer falsks were each filled with 50; 75 or 100 ml of test solutions at EC50 (10 mg/L) or EC100 (100 mg/L). In the Erlenmeyer flask filled with 50 ml test medium and 100 mg/L, it was noticed a 78.7 growth inhibition and the light intensity at the bottom of the flask was 5 uE/m^2/s. The results of test conducted 100 ml test medium and 100 mg/L test substance indicated 90.0 growth inhibition and a measured the light intensity at bottom of the flask of 3 uE/m^2/s. In case of the experiments conducted with 10 mg/L test substance in 50 ml and 100 ml media, the results indicates a percentage inhibition of 10.6 and 21 with the measured light intensities at the bottom of 40 uE/m2/s and 20 uE/m2/s, respectively.
The final test was conducted in 75 ml and light intensity of 110-120 uE/m2/s in order to reduce the growth inhibition caused by the coloration of test solutions.
both experiments were conducted under the same conditions as the final test.
The results of the experiment 1) indicate 38.5 % growth inhibition in the 10 mg/L group compared with the control group. the light intensity at the bottom of the concentration group was lower as comparedto that of the control.
From the experiment 2) a trend was observed that the reduction of test solution volume increased the light intensities at the bottom of flsk in both 10 and 100 mg/L concentration groups, which in turn reduced the inhibitory effect.
Growth inhibition was attibuted to the inhibition of photosynthesis and not to the effects of the substance on algae. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21.4 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Remarks:
- determined by least squared linear regression analysis
- Basis for effect:
- growth rate
- Remarks on result:
- other: 20.7-22.2
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.14 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: nominal was 3.2 mg/L
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- EC50: 0.698 mg/L (95% C.I. 0.660-0.739 mg/L), exposure period 8-11 Jun 2010
- Other: the average values of ErC50 since June 2000 were 0.815 +/- 0.085 mg/L, n=21
Min-max = 0.687 - 0.965 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance was a water-soluble and colored therefore causing the coloration of the test solutions. Growth inhibition resulting from the attenuation of light essential for the algal growth in the test solution was observed. The EC50 was 21.4 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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