D-Glucitol, 1,3:2,4-bis-O-[(4-chlorophenyl)methylene]-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Remarks:

see 'Principles of method if other than guideline'

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

yes

Remarks:

see 'Principles of method if other than guideline'

Principles of method if other than guideline:

The concentration of solvent in the treatments was 1.0 mL/L and therefore higher than stated in the guideline. As no inhibition was observed in the solvent control, this deviation was stated as uncritical. Additionally it was proven in several previously conducted experiments that a concentration of 1.0 mL/L acetone is uncritical for algal growth over a period of 72 hours.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(1R)-1-[(4R,4aR,8aS)-2,6-bis(4-chlorophenyl)-hexahydro-[1,3]dioxino[5,4-d][1,3]dioxin-4-yl]ethane-1,2-diol

EC Number:

700-591-1

Cas Number:

82203-23-4

Molecular formula:

C20H20Cl2O6

IUPAC Name:

(1R)-1-[(4R,4aR,8aS)-2,6-bis(4-chlorophenyl)-hexahydro-[1,3]dioxino[5,4-d][1,3]dioxin-4-yl]ethane-1,2-diol

Details on test material:

- Name of test material (as cited in study report): (1R)-1-[(4R,4aR,8aS)-2,6-bis(4-chlorophenyl)tetrahydro[1,3]dioxino[5,4-d][1,3]dioxin-4-yl]ethane-1,2-diol
- Expiration date of the lot/batch: December 2012
- Storage condition of test material: Room Temperature: (20 ± 5°C); Keep away from humidity

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.35 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

other: growth rate, cell number and biomass

Remarks on result:

other: test substance showed no algal cell growth inhibition up to its limit of solubility in water

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 0.35 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

other: growth rate, cell number and biomass

Remarks on result:

other: test substance showed no algal cell growth inhibition up to its limit of solubility in water

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The following results for the test item were determined:

Endpoint NOEC LOEC EC50
Growth Rate ≥ 0.35 mg/L > 0.35 mg/L > 0.35 mg/L
AUC ≥ 0.35 mg/L > 0.35 mg/L > 0.35 mg/L
Yield ≥ 0.35 mg/L > 0.35 mg/L > 0.35 mg/L

[0.35 mg/L = limit of solubility in test medium]

The test substance showed no algal cell growth inhibition up to its limit of solubility in water.