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EC number: 309-627-7 | CAS number: 100545-46-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2018-06-14 to 2018-10-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Copper, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, [[3-(cyclohexylamino)propyl]amino]sulfonyl derivs.
- EC Number:
- 309-627-7
- EC Name:
- Copper, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, [[3-(cyclohexylamino)propyl]amino]sulfonyl derivs.
- Cas Number:
- 100545-46-8
- Molecular formula:
- C32H16-τN8Cu.(SO3)m.(SO3H)p.(C9H19N2SO2)O.(C9H20N2SO2)n
- IUPAC Name:
- Copper, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, [[3-(cyclohexylamino)propyl]amino]sulfonyl derivs.
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 18-25°C, dark, in tightly closed original container, in evacuated desiccator
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
* 0 mg/L of test item (control)
* 100 mg/L of test item (limit test)
- Sampling method: At the start of the exposure and at the renewal (0 and 24 hours), monitoring samples of the fresh media were taken after preparation of the WSF and analyzed. Sampling of the old media was not carried out, since the test item concentration was below the LOD in the fresh media.
- Preparation of samples: The control and the sample were analysed undiluted.
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until the start of the analysis, if necessary. Prepared samples were stored in the autosampler at room temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Water Accommodated Fraction: In view of the difficulties associated with the evaluation of aquatic toxicity of poorly water soluble test items, a modification of the standard method for the preparation of aqueous media was performed. An approach endorsed by several important regulatory authorities in the EU and elsewhere (ECETOC 1996 and OECD 2000), is to expose the organisms to a Water Accommodated Fraction (WAF) of the test item in cases where the test item is a complex mixture, an UVCB substance and is poorly soluble in water and in the permitted solvents. Using this approach, aqueous media were prepared by mixing the test item with dilution water for a prolonged period sufficient to ensure equilibration between the test item and the water phase.
A water soluble fraction (WSF) was prepared 24 ± 1 hour prior to the start of the exposure (day -1) as well as prior to the renewal of the test solutions (day 0). For the preparation of the WSF, an appropriate amount of the test item was weighed and placed onto a glass slide. The glass slide with the test item was inserted in a brown glass flask filled with an appropriate amount of dilution water. This dispersion was shaken for 24 hours with 20 rpm at room temperature. Undissolved particles were removed by membrane filtration (membrane filter 0.20 µm, RC, MACHEREY-NAGEL). The filter was saturated in order to avoid adsorption during the filtration. The first 25 mL of the filtrate were discarded. The filtration was interrupted for 15 minutes to allow adsorption and saturation of the filter material with dissolved test item. Thereafter, the filtration was continued. The next 25 mL were discarded. The following filtrate, i.e. the water soluble fraction (WSF) was used in the test. During filtration, the filter was always kept covered with the test solution. The WSF was checked via laser beam (Tyndall effect) for undissolved test item. The Tyndall effect was negative.
- Controls: Dilution water without test item incubated under the same conditions as the test group.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUS
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
- Age of parental stock (mean and range, SD): less than 24 hours (at test initiation)
- Feeding during test: no
ACCLIMATION
- Acclimation period: Acclimatization of the daphnids was not necessary, because the composition of the dilution water was equivalent to the culture medium.
Study design
- Test type:
- semi-static
- Water media type:
- other: Medium Elendt M4 according to OECD 202, Annex 3 (2004)
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Dilution Water at the Start of the Exposure (0h): 211 mg CaCO3/L
Dilution Water at the Renewal (24 hours): 215 mg CaCO3/L - Test temperature:
- 18 - 22 °C, ± 1 °C
- pH:
- Fresh media at start of exposure (0 hours): 8.08 - 7.97 (100 mg/L - control)
fresh Media at the Renewal (24 hours): 7.70 - 7.95 (100 mg/L - control)
24-hours old Media at the Renewal (24 hours): 7.67 - 7.73 (100 mg/L - control)
at the End of the Exposure (48 hours) : 7.76 - 7.79 (100 mg/L - control)
Dilution Water at the Start of the Exposure (0 hours): 7.97
Dilution Water at Renewal (24 hours): 7.95 - Dissolved oxygen:
- Fresh media at start of exposure (0 hours): 8.23 mg/L - 9.25 mg/L (100 mg/L - control)
fresh Media at the Renewal (24 hours): 8.45 mg/L - 9.13 mg/L (100 mg/L - control)
24-hours old Media at the Renewal (24 hours): 8.10 mg/L - 8.33 mg/L (100 mg/L - control)
at the End of the Exposure (48 hours) : 8.91 mg/L - 8.72 mg/L (100 mg/L - control)
Dilution Water at the Start of the Exposure (0 hours): 9.25 mg/L
Dilution Water at Renewal (24 hours): 9.13 mg/L - Conductivity:
- Dilution Water at the Start of the Exposure (0h) : 522 µS/cm
Dilution Water at Renewal (24h) : 554 µS/cm - Nominal and measured concentrations:
- Nominal concentration : 100 mg/L of test item
Measured concentration: measured concentration of the test item in the fresh media at the start of the exposure (0 hours) and at the renewal (24 hours) was below the limit of detection (LOD = 0.2 mg/L), the analytical monitoring at the end of the exposure was waived and the test item concentration was considered to be below the LOD in the 24-hours old media. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity, loosely covered with watch glasses
- Type : closed with watch glasses
- Material, size, headspace, fill volume: 50 mL glass beaker, filled at 20 mL
- Aeration: No
- Renewal rate of test solution: The test solutions were renewed after 24 hours. For this purpose, a second set of preconditioned test vessels was filled with the freshly prepared test solutions and the daphnids were be transferred by pipette
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 1500 lx
RANGE-FINDING STUDY
- Test concentrations:
* 0 mg/l of test item (control)
* 100 mg/l of test item
- Results used to determine the conditions for the definitive study: A preliminary range finding test was conducted at the test facility under semi-static conditions over a period of 48 hours with three WSFs with nominal test item loadings of 1, 10 and 100 mg/L prepared with dilution water. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate p.a. (SIGMA)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EL10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: nominal test item loading
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: nominal test item loading
- Duration:
- 24 h
- Dose descriptor:
- EL100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: nominal test item loading
- Duration:
- 48 h
- Dose descriptor:
- EL10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: nominal test item loading
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: nominal test item loading
- Duration:
- 48 h
- Dose descriptor:
- EL100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: nominal test item loading
- Details on results:
- - Behavioural abnormalities: non observed
- Mortality of control:non observed - Results with reference substance (positive control):
- - Results with reference substance valid: Yes
EC50 (24 hours): 0.6 - 2.4 mg/L, according to AQS P 9/2 (clone 5)
EC50 (24 hours): 0.6 - 2.1 mg/L, according to OECD 202 (clone A) - Reported statistics and error estimates:
- An EC50-value was calculated for the reference item by sigmoidal dose-response regression. The respective confidence limits for the EC50 were empirically derived from the observation data as follows: The highest concentration level without any effect (EC0) and the lowest concentration level causing 100% immobilization (EC100) were used as confidence limits, since calculation was not possible by the software. All calculations were carried out from the best-fit values with the software GraphPad Prism.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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