Registration Dossier
Registration Dossier
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EC number: 284-395-7 | CAS number: 84870-65-5
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987-10-30 to 1988-01-26
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 28 412, part 15
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-[[4-(diethylamino)-2-methylphenyl]azo]-5-nitrobenzene-1,3-dicarbonitrile
- EC Number:
- 284-395-7
- EC Name:
- 2-[[4-(diethylamino)-2-methylphenyl]azo]-5-nitrobenzene-1,3-dicarbonitrile
- Cas Number:
- 84870-65-5
- Molecular formula:
- C19H18N6O2
- IUPAC Name:
- 2-[[4-(diethylamino)-2-methylphenyl]azo]-5-nitrobenzene-1,3-dicarbonitrile
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Leuciscus idus melanotus
- Details on test organisms:
- TEST ORGANISM
- Common name: Goldorfe
- Length at study initiation (length definition, mean, range and SD): 5 - 8cm
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 15 +/- 3°
- Test temperature:
- 20 +/- 1°C
- pH:
- at test start: 8.5
- Dissolved oxygen:
- > 5mg O2/L
- Nominal and measured concentrations:
- 5 g/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: filled volume: 10L
- No. of organisms per vessel: 10
- Biomass loading rate: 0.8 - 1.1 g/cm³
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: mixture of chlor free mains water and deionised water
- Ca/Mg ratio:4:1
OTHER TEST CONDITIONS
- Photoperiod: 12 - 16h light per day
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 5 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- not determinable
- Details on results:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: product is nearly insoluble, sedimented
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test item caused no mortality during the 48 h test. The test item is nearly insoluble in the test medium.
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