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EC number: 279-903-9 | CAS number: 82136-26-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 OCT 2013 - 14 JAN 2014 (experimental phase)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- of Commission Regulation (EC) No 440/2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- as of April 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 mg/L (control) and 100 mg/L
- Sampling method: Water samples were taken from one replicate of the control and all replicates of the 100 mg/L treatment, samples from treatment replicates were pooled, at the start (0 h) and end of the test (48 h)
- Sample storage conditions before analysis: samples were stored frozen and thawed with the aid of sonication prior to analysis - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 200 mg was dissolved in test water and the volume adjusted to 2 L to give the 100 mg/L test concentration. The test concentration was inverted several times to ensure adequate mixing and homogeneity.
- Controls: one blank control
- Test concentration separation factor: none, only one concentration
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no, solutions were clear and colourless - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: not stated
- Age at study initiation (mean and range, SD): less than 24 hours old
- Stage and instar at study initiation: 1st instar
- Method of breeding: Adult Daphnia were maintained in 150 mL glass beakers containing Elendt M7 medium in a temperature-controlled room at approximately 20 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods. Each culture was fed daily with a mixture of algal suspension (Desmodesmus subspicatus) and Tetramin® flake food suspension. Culture conditions ensured that reproduction was by parthenogenesis.
- Source: inhouse lab culture
- Feeding during test: no
ACCLIMATION
- No acclimation described - Test type:
- static
- Water media type:
- freshwater
- Remarks:
- Reconstituted water (ISO medium)
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- approx. 20°C (room temperature)
- pH:
- Test start: 7.3 (control) and 7.9 (100 mg/L)
Test end: 7.6 (control) and 8.1 (100 mg/L) - Dissolved oxygen:
- 9.0 mg O2/L throughout the test
- Nominal and measured concentrations:
- 100 mg/L (nominal)
99.3 mg/L (measured, 0 h)
97.7 mg/L (measured, 48 h) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass jars
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass jars, 250 mL, filled with 200 mL test solution
- Volume of solution: 200 mL
- Aeration: none
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: preparation before the test start, composition details under 'Any other information', Table 1
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: yes, pH of test solutions was adjusted to pH 7.9 prior to addition of daphnids
- Photoperiod: 16:8 light:dark
- Light intensity: 630 to 685 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilisation, every 24 hours
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes, no immobilisation was observed up to 100 mg/L in the second trial (in the first trial complete immobilisation at 100 mg/L was observed, but the first trial was invalid as the pH value of the 100 mg/L test solution was outside the range of pH 6-9) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Immobilisation of control: After 24 hours, one out of the 5 daphnids in one replicate of the control was observed to be trapped at the surface, however, as this observation was no longer present after 48 hours, this was not considered to have an effect on the outcome of the test.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L
- Limit test: no
- Dose-response test: yes
- ECx (95% CI): 24h-EC50 = 1.0 mg/L (0.91-1.2 mg/L), 48h-EC50 = 0.71 mg/L (0.65-0.76 mg/L) - Reported statistics and error estimates:
- No statistical evaluation; EC50 values were estimated by inspection of the immobilisation data.
- Validity criteria fulfilled:
- yes
- Remarks:
- Control mortality below 10% (0%) and oxygen concentration at test end higher than 3 mg/L (9 mg/L)
- Conclusions:
- The study was performed under GLP according to the guidelines OECD 202 (2004) and EU-Method C.2, without deviations which may have impact on the validity of the study. Thus, the results were obtained via a scientifically reasonable method. Hence, there is no doubt that the obtained results are not reliable. According to the reported 48h-EC50 value of >100 mg/L the test substance possesses no toxic potential towards aquatic invertebrates.
- Executive summary:
In the present study the acute toxicity of the test item towards aquatic invertebrates was investigated according to relevant OECD test guideline 202 and EU-Method C.2, without deviations which may have impact on the validity of the study. The study was performed under GLP conditions and a GLP certificate is included in the final report.
Daphnia magna was used as test organisms. 20 animals per treatment were exposed to the test substance at a concentration of 100 mg/L for 48 hours in a static test. A blank control was included and the reference substance potassium dichromate was tested at regular intervals. Under the conditions used for the test, the following effect concentrations were determined based on immobilisation:
48h-EC50 > 100 mg/L
As a conclusion of the analytical part of this study, a slight change in the concentration of the test substance in the test solutions at test start and test end was observed, but the recoveries were still well above 90%. Therefore, the biological results were based on the nominal concentrations of the test substance.
Reference
Description of key information
acute toxicity of substance 2-[[3-(dimethylamino)propyl]methylamino]ethanol to freshwater invertebrates Daphnia magna (OECD 202):
48h-EC50: >100 mg/L (nominal, based on mobility)
48h-NOEC: 100 mg/L (nominal, based on mobility)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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