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EC number: 268-329-4 | CAS number: 68052-51-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 May 2017 - 03 August 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from all concentrations and the control according to the schedule below:
Frequency: at t=0 h and at t=48 h
Volume: 2.0 mL
Storage: Samples were stored in a freezer (≤ 15°C) until analysis.
At the end of the exposure period, the replicates were pooled at each concentration before sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct application to the test medium: preparation of test solutions for the range-finding test and the final test started with the highest concentration of 100 mg/L (37 mg anion/L). Since the test item was completely soluble in test medium, no other treatment than vigorous shaking was needed to completely dissolve the test item in medium. Lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium.
- Controls: Test medium without test item or other additives.
- Evidence of undissolved material: All test solutions were clear and colorless at the end of the preparation procedure. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia
- Age at study initiation: <24 hours
- Source: in-house laboratory culture with a known history, at least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions. The daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
- Age of parental stock: >2 weeks
- Feeding during test: no
BREEDING
- Breeding method: each batch was started with newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium (M7) in an all-glass culture vessel. Cultures (maximum age of 4 weeks) were renewed after 7 days of cultivation by renewing the medium twice a week.
- Feeding: daily, a suspension of fresh water algae.
- Health during breeding period (any mortality observed): no mortality observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 180 mg CaCO3/L
- Test temperature:
- 20 - 21°C
- pH:
- Start: 7.9-8.0
End: 8.2-8.3 - Dissolved oxygen:
- Start: 8.9 mg O2/L
End: 8.8-9.1 mg O2/L - Nominal and measured concentrations:
- Nominal concentrations: 10, 18, 32, 56 and 100 mg test item/L (corresponding to 3.7, 6.6, 12, 21 and 37 mg anion/L)
Measured concentrations at t=0: 10, 18.5, 32.0, 57 and 100 mg test item/L based on the anion concentration and 10, 19.0, 33.4, 58.1 and 106 mg test item/L based on cation concentration
Concentrations at t=72 were 98-102% of initial test solutions based on the anion concentration and 96-101% of initial based on the cation concentration.
Based on these results the effect concentrations were based on the nominal concentrations mg test item/L calculated from the nominal concentrations of anion. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 60 mL, all-glass vessels, open, fill volume: 50 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water purified by Reverse Osmosis
- Culture medium different from test medium: yes, M7 medium
- Intervals of water quality measurement: at t=0 h and at t=48 h (dissolved oxygen and pH measurements)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours daily
EFFECT PARAMETERS MEASURED: immobility at t=24 and t=48 hours
COMBINED LIMIT/RANGE-FINDING STUDY
- Test concentrations: 0.10, 1.0, 10 and 100 mg/L (0.037, 0.37, 3.7 and 37 mg anion/L)
- Results used to determine the conditions for the definitive study: yes, in the highest concentration 60% of the daphnids became immobilized after 24 hours and 100% was immobilized after 48 hours. The expected EC50 was between 10 and 100 mg test item/L. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (June 2017)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 34 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 30-40 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: anion
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 11-15 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: cation
- Basis for effect:
- mobility
- Remarks on result:
- other: cation concentration calculated
- Details on results:
- - Any abnormalities observed: no
- No immobilisation was observed in the control and the lowest concentration tested. A dose-related increase of immobilisation was observed at the end of the test.Microscopic observations at t=24 h (in one replicate vessel) showed that there was no test item attached to the daphnids exposed to the highest concentration. It was therefore concluded that the observed immobility was not due to mechanical obstruction.
- Actual exposure concentrations were confirmed by analysis at the start and at the end of the test. However, 3 out of 5 concentrations were calculated by extrapolation of the calibration curve of the anion. Since cation results where acceptable (the same results with the curve with broader range) and not extrapolated, all values for anion concentrations are accepted without additional analysis.
- Analysis showed that measured concentrations of the anion were 98-102% of nominal concentrations after the 72 hour exposure period. Effect parameters were expressed in terms of analytically confirmed nominal concentrations of the anion and calculated to mg test item/L. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Concentrations tested: 0.10, 0.18, 0.32, 0.56, 1.0 and 1.8 mg/L
- Relevant effect levels: 48h-EC50: 0.59 mg/L, 95% CI: 0.52-0.66 mg/L
- Other: the results where within the expected range according to the historical data (>0.3 and <1.0 mg/L) - Reported statistics and error estimates:
- The 24 and 48h-EC50-value was calculated from the probits of the percentages of affected daphnids and the logarithms of the corresponding nominal concentrations of the anion using the maximum likelihood estimation method.
ToxRat Professional v 3.2.1 (ToxRat Solutions® GmbH, Germany) was used to perform the analyses. - Validity criteria fulfilled:
- yes
- Remarks:
- see 'overall remarks'
- Conclusions:
- The 48h-EC50 for Daphnia magna exposed to TBEAES DRY was 34 mg/L based on the nominal concentration of the test item.
- Executive summary:
In a short-term toxicity test, performed according to OECD guideline 202 and GLP principles, TBEAES DRY was assessed for its potential toxicity towards Daphnia magna. The test item concentration was corrected for the concentration of anion with a correction factor of 0.368.
Test solutions were prepared by adding the test item directly to the test medium and shaking the solutions vigorously for complete dissolution of the test item. A combined limit/range-finding test was performed with test concentrations of 0.1, 1.0, 10 and 100 mg/L (0.037, 0.37, 3.7 and 37 mg anion/L).
In the final test twenty daphnids were exposed to a blank control and test item concentrations of 10, 18, 32, 56 and 100 mg/L (3.7, 6.6, 12, 21 and 37 mg anion/L) under static conditions for 48 hours. Samples were taken from the control and all concentrations at the start and at the end of the test for confirmation of exposure concentrations. The analysis was based on the concentration of anion and cation in the test solutions. Test item concentrations were calculated from the anion and cation concentrations and results for the two were comparable. Measured concentrations of the anion were 98-102% of nominal concentrations after the 72 hour exposure period and 96 -101% for the cation. Effect parameters were expressed in terms of analytically confirmed nominal concentrations of the anion and calculated to mg test item/L.
Incidence of immobility was recorded at t=24 and t=48. The 48h-EC50 for Daphnia magna exposed to TBEAES DRY was determined to be 34 mg test item/L (13 mg anion/L). The acceptability criteria were met and the study was considered to be valid.
Reference
Table 1 Measured concentrations (anion) versus nominal concentrations
Time of sampling [hours] |
Concentration |
Relative to nominal |
Relative to initial [%] |
|
Nominal |
Analyzed |
|||
0 |
0 |
< 1 |
n.a. |
|
|
10 |
10*,# |
101 |
|
|
18 |
18.5 |
103 |
|
|
32 |
32.0 |
100 |
|
|
56 |
57*,# |
101 |
|
|
100 |
100*,# |
100 |
|
48 |
0 |
< 1 |
n.a. |
n.a. |
|
10 |
10*,# |
100 |
99 |
|
18 |
18.4 |
102 |
99 |
|
32 |
32.7 |
102 |
102 |
|
56 |
57*,# |
102 |
101 |
|
100 |
98*,# |
98 |
98 |
* Estimated value, calculated by extrapolation of the calibration curve.
# Since cation results where acceptable (the same results with the curve with broader range) and not extrapolated, anion results are accepted without additional analysis.
n.a. Not applicable.
Table 1 Measured concentrations (cation) versus nominal concentrations
Time of sampling [hours] |
Concentration |
Relative to nominal |
Relative to initial [%] |
|
Nominal |
Analyzed |
|||
0 |
0 |
< 0.1 |
n.a. |
|
|
10 |
10.0 |
100 |
|
|
18 |
19.0 |
106 |
|
|
32 |
33.4 |
104 |
|
|
56 |
58.1 |
104 |
|
|
100 |
106 |
106 |
|
48 |
0 |
< 0.1 |
n.a. |
n.a. |
|
10 |
9.97 |
100 |
99 |
|
18 |
19.0 |
105 |
100 |
|
32 |
33.6 |
105 |
101 |
|
56 |
58.7 |
105 |
101 |
|
100 |
102 |
102 |
96 |
n.a.Not applicable.
Table 2 Number of introduced daphnids and incidence of immobility in the final test
Time (h) |
Replicate |
Nominal concentration TBEAES DRY (mg anion/L) |
|||||
Control |
3.7 (10) |
6.6 (18) |
12 (32) |
21 (56) |
37 (100) |
||
0 |
A |
5 |
5 |
5 |
5 |
5 |
5 |
B |
5 |
5 |
5 |
5 |
5 |
5 |
|
C |
5 |
5 |
5 |
5 |
5 |
5 |
|
D |
5 |
5 |
5 |
5 |
5 |
5 |
|
Total introduced |
20 |
20 |
20 |
20 |
20 |
20 |
|
24 |
A |
0 |
0 |
0 |
0 |
0 |
1 |
B |
0 |
0 |
0 |
0 |
1 |
2# |
|
C |
0 |
0 |
0 |
0 |
0 |
1 |
|
D |
0 |
0 |
0 |
0 |
1 |
1 |
|
Total immobilised |
0 |
0 |
0 |
0 |
2 |
5 |
|
Effect % |
0 |
0 |
0 |
0 |
10 |
25 |
|
|
|
|
|
|
|
||
48 |
A |
0 |
0 |
0 |
1 |
4 |
5 |
B |
0 |
0 |
0 |
2 |
5 |
5 |
|
C |
0 |
0 |
0 |
2 |
5 |
5 |
|
D |
0 |
0 |
1 |
2 |
5 |
5 |
|
Total immobilised |
0 |
0 |
1 |
7 |
19 |
20 |
|
Effect % |
0 |
0 |
5 |
35 |
95 |
100 |
() – between brackets, the concentration of the test item are given (mg/L),#Microscopic observation revealed no test item attached to the daphnids
Description of key information
In a short-term toxicity test, performed according to OECD guideline 202 and GLP principles, TBEAES DRY was assessed for its potential toxicity towards Daphnia magna. The test item concentration was corrected for the concentration of anion with a correction factor of 0.368. Test solutions were prepared by adding the test item directly to the test medium and shaking the solutions vigorously for complete dissolution of the test item. A combined limit/range-finding test was performed with test concentrations of 0.1, 1.0, 10 and 100 mg/L (0.037, 0.37, 3.7 and 37 mg anion/L). In the final test twenty daphnids were exposed to a blank control and test item concentrations of 10, 18, 32, 56 and 100 mg/L (3.7, 6.6, 12, 21 and 37 mg anion/L) under static conditions for 48 hours. Samples were taken from the control and all concentrations at the start and at the end of the test for confirmation of exposure concentrations. The analysis was based on the concentration of anion and cation in the test solutions. Test item concentrations were calculated from the anion and cation concentrations and results for the two were comparable. Measured concentrations of the anion were 98-102% of nominal concentrations after the 72 hour exposure period and 96 -101% for the cation. Effect parameters were expressed in terms of analytically confirmed nominal concentrations of the anion and calculated to mg test item/L. Incidence of immobility was recorded at t=24 and t=48. The 48h-EC50 for Daphnia magna exposed to TBEAES DRY was determined to be 34 mg test item/L (13 mg anion/L). The acceptability criteria were met and the study was considered to be valid.
Key value for chemical safety assessment
Additional information
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