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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study was carried out according to test guidline and under GLP-conditions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
N-(4-acetylaminophenyl)-3-hydroxynaphthalene-2-carboxamide
EC Number:
255-419-3
EC Name:
N-(4-acetylaminophenyl)-3-hydroxynaphthalene-2-carboxamide
Cas Number:
41506-62-1
Molecular formula:
C19H16N2O3
IUPAC Name:
N-(4-acetamidophenyl)-3-hydroxy-2-naphthamide
Details on test material:
- Name of test material (as cited in study report): BONS-p-acetaminoanilid TTR9 (Naphtol AS-ONSAP)
- Lot/batch No.: production 1989 (Pt. 43/89 + Pt. 58/89)
- Analytical purity: 98.8 %

Test solutions

Vehicle:
yes
Details on test solutions:
Tween 80 used as vehicle. Additionally a solvent control was done with 0.095 ml Tween 80/1

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
static
Total exposure duration:
96 h

Test conditions

Test temperature:
21.4 - 22.5°C
pH:
7.7 -8.1
Dissolved oxygen:
5.3 - 8.7 mg/L
Nominal and measured concentrations:
0/500 mg/L (nominal)
0/71.3 mg/L (mean of measured conc.)
Details on test conditions:
use of reconstituted water, composition acc. to ISO/DIS 7346/1
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: lethality value based on loading rates (nominal conc.); mean value of measured concentrations: 71.3 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: lethality value based on loading rates (nominal conc.); mean value of measured concentrations: 71.3 mg/L
Details on results:
0 of 10 fish showed any change of appearance and mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
67/548/EEC: Acute aquatic toxicity: no classification
1272/2008/EC: Acute aquatic toxicity: no classification
Executive summary:

No lethality was observed up to the solubility limit of Naphtol AS-ONSAP. The 500 mg/L (71.3 mg/L*) group exhibited no symptoms for appearance and mobility.

* = value based on the mean of analytically determined values