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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989-1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: according to OECD 302B (Zahn- Wellens test) but only results reported

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Qualifier:
according to guideline
Guideline:
EU Method C.9 (Biodegradation: Zahn-Wellens Test)
Qualifier:
according to guideline
Guideline:
other: german DIN 38412
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
N-(4-acetylaminophenyl)-3-hydroxynaphthalene-2-carboxamide
EC Number:
255-419-3
EC Name:
N-(4-acetylaminophenyl)-3-hydroxynaphthalene-2-carboxamide
Cas Number:
41506-62-1
Molecular formula:
C19H16N2O3
IUPAC Name:
N-(4-acetamidophenyl)-3-hydroxy-2-naphthamide

Study design

Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Details on inoculum:
activated sludge from industrial source; no further detailed information about inoculum
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
114 other: mg C/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Preparation of stock solution:
test substance 5 g/L
pH adjusted to 7.0
batch agitated for 24h at 21°C
pH adjusted to 7.0
DOC determined value of stock solution: 150 mg C/L (calculated theoretical value.: 712.8 mg C/g)
COD determined value of stock solution: 418 mg O2/L (calculated theoretical value.: 1998 mg O2/g)
Reference substance
Reference substance:
not specified

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
3 h
Remarks on result:
other: determined elimination (biodegradation + adsorption) of 75% fully caused by adsorption --> biodegradation = 0%
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
5 d
Remarks on result:
other: determined elimination (biodegradation + adsorption) of 75% fully caused by adsorption --> biodegradation = 0%
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
12 d
Remarks on result:
other: determined elimination (biodegradation + adsorption) of 63% fully caused by adsorption --> biodegradation = 0%
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
21 d
Remarks on result:
other: determined elimination (biodegradation + adsorption) of 78% fully caused by adsorption --> biodegradation = 0%
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Remarks on result:
other: determined elimination (biodegradation + adsorption) of 83% fully caused by adsorption --> biodegradation = 0%

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The tested substance is not biodegradable.