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EC number: 235-101-0 | CAS number: 12068-53-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 Jun 2016 - 13 Jul 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Ministry of environmental protection of the people´s Republic of China, The Guidelines for the Testing of Chemicals - Effects on Biotic Systems, 207 Earthworm, Acute Toxicity Test
- Version / remarks:
- 2013
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil: An amount of the test substance was separately added to a certain amount of silica sand and then incorporated into formulated artificial soil with deionized reverse osmosis water. The test matrix components (2520.2 mg test substance, 41.7 g silica sand, 600 mL water and 2478 g dry artificial soil) were mixed by a mixer with a nominal moisture of 40-60%. The test matrix components were mixed evenly and distributed into 4 containers. Each container was loaded with approx. 750 g wet weight of the substrate. The same procedure was followed for the blank control without adding the test substance.
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: Jiang Su province Jurong Stock Farm. All acquired earthworms were observed for 14 d prior to use.
- Age at test initiation (mean and range, SD): adult earthworms (with clitellum)
- Weight at test initiation (mean and range, SD): 300 - 600 mg
ACCLIMATION
- Acclimation period and conditions: Earthworms were bred on a substrate consisting of 50% peat and 50% cow manure and kept under 20±2°C for 7 d prior testing. Test organisms were then transferred into artificial soil (without test substance) approx. 24 h prior to start of the test. - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 20.3 - 20.5 °C
- pH:
- 6.22 - 6.33
- Moisture:
- Day 0: 28.57% in the blank control and 29.91% in the treatment group
Day 14: 27.95% in the control and 28.73% in the treatment group - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1 L closed glass vessel.
- Amount of soil or substrate: 750 g wet weight substrate.
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- % sphagnum peat: 10% w/w
- % sand: 70% w/w
- % kaolinite clay: 20% w/w
- Maximum water holding capacity (in % dry weight): 52% (determined according to OECD 222).
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity: 603 - 625 lux
EFFECT PARAMETERS MEASURED:
- Mortalities were recorded after 7 and 14 d. Total gross weight of the earthworms was recorded on Day 0. Surviving earthworms were weighed on Day 14.
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0 (control), 10, 100, 1000 mg/kg (no replicates)
- Results used to determine the conditions for the definitive study: Based on the results of the range finding study (no mortalities within 14 d), one treatment group and one blank control group which had four replicates of 10 worms per replicate were included in the limit test (1000 mg/kg). - Nominal and measured concentrations:
- Nominal concentration: 1000 mg/kg soil dw
- Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide, determined once a year (most recent test 19 Jan - 2 Feb 2016, 14 d test with concentrations of 3.75, 7.5, 15, 30 and 60 mg/kg)
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period:
0% on Day 7 and 2.5% on Day 14.
- Mortality in control group at the end of exposure: 0%
- Total mass of adults at beginning of test: 447 - 459 mg in blank control and 467 - 493 mg in treatment group.
- Changes in body weight of live adults (% of initial weight) at end of exposure period: 8 ± 5.34% in control and 10.39 ± 4.34 % in treatments.
- Morphological abnormalities: no
- Behavioural abnormalities: no
- Other biological observations: no - Results with reference substance (positive control):
- - Results with reference substance valid: yes
- Relevant effect levels: LC50 (14 d) = 25.7 mg/kg soil dw - Validity criteria fulfilled:
- yes
Reference
Table 1: Mortality of E. fetida exposed to the test substance and controls after 7 and 14 days.
Parameter | Blank control | Treatments | ||
Duration [d] | 7 | 14 | 7 | 14 |
Number of earthworms | 40 | 40 | 40 | 40 |
Number of mortality total | 0 | 0 | 0 | 1 |
Mortality % | 0 | 0 | 0 | 2.50% |
No pathological or behavioural symptoms were observed.
Table 2: Average body weight of the surviving earthworms on Day 0 and 14.
Groups | Day 0 | Day 14 | Weight reduction 14 d (%)± standard deviation | ||
No of worms | Mean weight [mg] | No of worms | Mean weight [mg] | ||
Blank control | 10 | 452 | 10 | 439 | 8± 5.34% |
10 | 459 | 10 | 404 | ||
10 | 484 | 10 | 465 | ||
10 | 447 | 10 | 388 | ||
Treatment | 10 | 489 | 10 | 407 | 10.39± 4.34 % |
10 | 467 | 10 | 434 | ||
10 | 493 | 9 | 450 | ||
10 | 490 | 10 | 446 |
Description of key information
LC50 (14 d) > 1000 mg/kg soil dw (nominal, Eisenia fetida, OECD 207)
Key value for chemical safety assessment
Additional information
One study is available testing the toxicity of the substance towards earthworms (2016). The study was conducted according to OECD guideline 207 and GLP standards. The earthworm species Eisenia fetida was exposed to a single nominal test substance concentration of 1000 mg/kg soil dw and a control for 14 d. No mortalities were observed in the control. The study resulted in an LC50 (14 d) of > 1000 mg/kg soil dw (nominal).
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