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EC number: 235-101-0 | CAS number: 12068-53-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 Feb - 23 Feb 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- The temperature in the pre-test was in a range of 16.9 - 18.8 °C instead of 20 ± 2 °C as recommeded by the guideline.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Pretreatment: Upon arrival in the test facility, the sludge was filtrated, washed with tap water and resuspended in tap water. The activated sludge was aerated until usage in the test and fed daily with 50 mL/L synthetic sewage feed. On the day before the experiment, the inoculum was taken from its source, washed, aerated and the dry matter was determined. The volume was adapted to the desired content of dry matter. The nutrient solution was thawed and the sludge was fed with 50 mL/L sludge
- Dry matter of sludge: 2.50 g suspended solids/L
- Dry matter in the test: 1.25 g suspended solids/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 1.08 mmol/L
- Test temperature:
- 16.9 - 18.8 °C
- pH:
- 7.7 - 7.8
- Nominal and measured concentrations:
- nominal: 0, 1.2, 12.2, 100, 999.4, 1000 and 1001.2 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, fill volume: glass beaker, 1000 mL, fill volume: 500 mL
- Aeration: purified air, using Pasteur pipettes, flow approx. 0.75 L/min
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 6
- Biomass loading rate: 1.25 g suspended solids/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised tap water
- Total organic carbon: 0.5 mg/L
- Pesticides: < limit of detection
- Chloride: 12.0 mg/L
- Ca: 35.7 mg/L
- Mg: 0.007 mg/L
- Conductivity: 223 µS/cm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : respiration rate, at the end of the test
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 8 to 10 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichloro-phenole
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No statistically significant inhibition of respiration was observed at any of the treatments.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 of 8.1 mg/L
Reference
Description of key information
NOEC >= 1000 mg/L (activated sludge, OECD 209)
Key value for chemical safety assessment
Additional information
One study on the toxicity of Zinc aluminium oxide to microorganisms is available. This key study was performed as a limit test according to OECD Guideline 209, using activated sludge as inoculum (2012). The sludge was exposed to nominal concentrations of 0 (control), 1.2, 12.2, 100, 999.4, 1000 and 1001.2 mg/L during 3 hours. As no significant inhibition was observed in any of the treatments, the NOEC was determined to be 1000 mg/L and the EC50 > 1000 mg/L.
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